Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,559	C→T	A319A (GCC→GCT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,559	0	C	T	94.6%	90.6 / NA	38	A319A (GCC→GCT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/20);  minor base G (0/2);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTT  >  NC_000913/4620312‑4620794                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            cgccAGCACCGACCGTGCTTTAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCtgcg                                                                                                                                                                                                                                          <  1:189831/251‑1 (MQ=255)         ccgaccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAGCATCGGCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGa                                                                                                                                                                                                                                  <  1:192047/251‑1 (MQ=255)           gaccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGagc                                                                                                                                                                                                                                >  1:219151/1‑249 (MQ=255)             ccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTAGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTg                                                                                                                                                                                                                              >  1:19631/1‑251 (MQ=255)                                                               gatAAAATTGCCTACATATACGCCAACTGCGGTATCACAAAGAACGAGAATGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGCTAACACCATCGTCTTCACCACCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGCCGCAGGTTATATTTCGGGTATGGAGCTGGTCGACCACAGTATGCCGGAGCTGACGTCTCTTCTTCGCGATgcagacg                                                                                                                                                                             >  2:340567/3‑244 (MQ=255)                                                                    aaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTg                                                                                                                                                                        >  1:171088/1‑250 (MQ=255)                                                                    aaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGGTTGACGCCACCATAAAAGAGATGAAAGACGCGGGGGATAACACCATCGTCTTCACCAACTTCGTTGACTTAGACTCTTCCTGGGGCCTCCGTAGCGACGTCGACGTTTATGCAGCGGGTCTGGAGCTGTTCGACCGACGTCTGCCGGAGCTGATGGCTCTGCTGCGCGagtacgccctacggt                                                                                                                                                                       >  2:4978/1‑237 (MQ=255)                                                                         cagACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTc                                                                                                                                                                  >  1:55212/3‑251 (MQ=255)                                                                                  aCGCCAACTGCGGTATCACCAATAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCt                                                                                                                                                            <  1:379881/248‑1 (MQ=255)                                                                                  aCGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAATAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGTCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCActgc                                                                                                                                                             <  1:362149/247‑4 (MQ=255)                                                                                     ccAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCAc                                                                                                                                                      >  1:83880/1‑251 (MQ=255)                                                                                     ccAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCAc                                                                                                                                                      <  1:11820/251‑1 (MQ=255)                                                                                        aCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGGGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTTCTGGCCACGGt                                                                                                                                                   >  2:109560/1‑251 (MQ=255)                                                                                           gCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgt                                                                                                                                                <  1:142992/251‑2 (MQ=255)                                                                                           gCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgt                                                                                                                                                <  1:13019/251‑2 (MQ=255)                                                                                             ggTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGCGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGCTGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgtg                                                                                                                                               >  1:22146/1‑248 (MQ=255)                                                                                               tATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATc                                                                                                                                            >  1:322595/1‑251 (MQ=255)                                                                                                               aaaGCCACTTGCCTGGCAGCGCGGTTTGACGCCAACATCAAAGAGATGAAAGAAGCGGGTGATCACACCACCGTCTTCCCCAACTTCGATGATTTCGACTCTTCCTGGTGCCCCCGTCGCGACGTCTCCGGTTATGCTGCGGGTTTGGAGCTGTTCGACCTCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCTCACTGCTGAACACGGTTGTGATCCGACCTGGACCGGTAc                                                                                                                            <  2:313362/251‑1 (MQ=255)                                                                                                                    aaCTGGTCTGGACGCGCTGTGTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGTTAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGTTACTGAcc                                                                                                                       <  2:53314/250‑1 (MQ=255)                                                                                                                        ggCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACcaca                                                                                                                    <  1:342911/250‑1 (MQ=255)                                                                                                                                            cGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTAc                                                                                               >  1:365885/1‑251 (MQ=255)                                                                                                                                                  caTCAAAGAGATGAAAGAATCGGTTGATAACCCCAGCGTCGACTCCAACTTCGTTGACTTCGAATCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGGCCTCCGTCTGCCGGATCTGATGGCTCTGCTTCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACAGTCCGGTACTGGttt                                                                                         <  2:322595/251‑3 (MQ=255)                                                                                                                                                   aTCAAAGAGAAGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCAGCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGTCATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGttta                                                                                        <  1:47358/251‑4 (MQ=255)                                                                                                                                                    acaataagaTGAAAGAATGGGTTGATAACGCCATCGTCTTCACCAACTTCGTTGTCTTCGACTCTTCCTGGGGCCCCCGTCGCGACGTCGCCGGTTATGCTTCGGGTCTGGAGCTGTTCGGCCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTTATCCTCACTGCTGACCACGGTTGTGATCCGTCCTGGACCGGTACTGACCACACTCGTGAACACATTCCGGTAcggggttac                                                                                       <  2:159809/245‑9 (MQ=255)                                                                                                                                                         tcgataaaaGAAACGGGAGATAACACCATCGTCTTCCCCAACTTCGTTGACTTCGACTCTTCCTGGGACCACCGTCGCGACGTCGCCGGTTATGCTGCTGGACTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGGAGACGACATCCTGATCCTCAGTGAGGACCACGGGTGTGATCCTGCCTGGACCGTGACTGACCACACGCGTGAACACATTCCGGTACTgggttacggacc                                                                                  >  2:71523/7‑241 (MQ=255)                                                                                                                                                         gagaTGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTGCGTTGACTTCGACTCTTCCTGGGGCCACCGGCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGccc                                                                                  <  1:57351/251‑1 (MQ=255)                                                                                                                                                                       gggTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCgg                                                                    >  1:137656/1‑251 (MQ=255)                                                                                                                                                                       gggTGATAACACCAGCGTCTTCACCACCTTCGTTGACTTCGACCCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCTGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGATCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCgg                                                                    <  1:71523/251‑1 (MQ=255)                                                                                                                                                                            aTAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCa                                                               <  1:38140/251‑1 (MQ=255)                                                                                                                                                                                ccccATCGTCTTCCCCAACGTCGTTGATTTCGACTCCTCCTGGGGCCACCGGCGCGACGTCGCCGGTTATTCTGCGGGTCTGGTGCGTTTCGACCGCCGTCTGCCGGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTgg                                                           <  2:291890/249‑1 (MQ=255)                                                                                                                                                                                       gTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACAGGGCTCACTGGGTCaccg                                                    >  1:216077/1‑248 (MQ=255)                                                                                                                                                                                           ccACCAACTGCTTTGATTTCGACCATGCCTGGGGCCCCCGTCGCGACGTCGCCGGTTAGTCTGCGGGTCTTGAGCTGTTCGACCTCCGTCTGCCGGAGCGGAGGTCTCTGCTGCGCGATGACGTCATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACAGTCCGGTACTGGTTTGCGGCCCGAAAGTAAAACCGGGCGCACTTGGTCACCGTg                                                  <  2:350041/248‑1 (MQ=255)                                                                                                                                                                                                cacttcggtgtgttCGGCTCTTCCCGGGGCCACCTACGGGACGTCGCAGGTTATGCTGCTGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACAGCCTGATCCTCACTGCTGTACACGTTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTTACCGTGAAACCtt                                           <  2:55212/239‑1 (MQ=255)                                                                                                                                                                                                 aCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                          <  1:46653/251‑1 (MQ=255)                                                                                                                                                                                                    tcggtgtcttggACTCTGCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCGGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCGGCTGCGCGATGACGACATCCTGATCCTCACTGCGGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                          <  2:83880/237‑1 (MQ=255)                                                                                                                                                                                                     cGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGATCGACCGCCGTCTGCCGGAGCTGCTGGCTCTGCTGCGAGATGACGACAGCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACAACCCGCGTGCACACTTTCAGGTACTGGCTGACAGCCCGAAAGGAAAACAGGGCTCACTGGGGCAACATGAAACCTACGCgg                                      >  2:194992/1‑251 (MQ=255)                                                                                                                                                                                                        tGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGATACCTTCGCggca                                    >  1:35403/1‑248 (MQ=255)                                                                                                                                                                                                                    tgccTGTGGCCACCTTCGCGGCGTGATCGGTTATGCTGCGGGGCTGTTGCTGGTCGACCGCAGTCTGCCGGAGTGGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct                        <  2:306294/248‑1 (MQ=255)                                                                                                                                                                                                                                         cggtggctgTTATGCGTCGGGTCTGGTGCTGTTCTACCGCCGGCTGCCGGAGCTGATGTCTCTGTTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACtt  <  2:311224/243‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTT  >  NC_000913/4620312‑4620794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑