Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,427	G→A	A275A (GCG→GCA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,427	0	G	A	96.9%	94.7 / NA	32	A275A (GCG→GCA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/14);  minor base C (0/1);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTAC  >  NC_000913/4620208‑4620672                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      cAACGGCGGCTACAACATCTGTCGTGTTATCGCTCGTCCGTTTTTCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGTCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGtcacca                                                                                                                                                                                                                        <  1:69963/251‑1 (MQ=255)        ggcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCaa                                                                                                                                                                                                                    >  1:53314/1‑248 (MQ=255)                           ttATCGCTCGTCCGTTTAGCGGTGACAAAGCCGTTAACTTCCAGCGTACCGGTACCCGCCACGCCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGa                                                                                                                                                                                               <  2:314005/250‑1 (MQ=255)                                gCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTAACGACCTGGCTGTTGAGCCGCCAGCACCGACAGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTATGTCGGTAAAATTGCAGAAATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAGGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGGGATAacac                                                                                                                                                                                         >  1:170132/1‑251 (MQ=255)                                   cGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAcc                                                                                                                                                                                        <  1:190136/249‑1 (MQ=255)                                       cgtttaccggtaaCAAAACCGGTACTTTCCCGCGTACCGGTAACCGTCACGACCTTGTTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCTCCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACAATCGTc                                                                                                                                                                                  <  2:92808/239‑1 (MQ=255)                                                   aaaaaGCCTGTAACTTCCTGGTTACCGGTAACCGCAACGGCCTGGATGGTGAGCCGCCAGTACCGACCGTGCTGCATAAACTGGTTTATGAAAAACACGGTCAGGTGGTGTCTGTCGGTAAAAGTGCAGACATCTACGCCATCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGGCTGTACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATACCACCATAGTCTTCACCAACTTc                                                                                                                                                                      <  2:56004/249‑1 (MQ=255)                                                          cGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACt                                                                                                                                                               >  1:12333/1‑251 (MQ=255)                                                                               tAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCAcc                                                                                                                                          >  1:291890/1‑251 (MQ=255)                                                                                  ccGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTc                                                                                                                                       <  1:67843/251‑1 (MQ=255)                                                                                                cTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGCCCACCGTCGCGACGTCGCCgtt                                                                                                                         >  2:11820/1‑249 (MQ=255)                                                                                                    tGAGCCGCCAGCAACGACAGTGCTGCAGAAACTGGTTGATGAAAAACACACTCAGGTGGTTTCTGACGGTACAATTGCAGACAACTACGCCAACTGCGGCATCACCACGCACGAGAAAGCAACTGGCCTGGACGCGCTGTTTGCCGCCACCATCAAAGAGATGAAAGACGCGGGTGATAACACCATCGTCTTAACCAAATTCGTTGACTTCGACATTTCCTGGAGCCACCGTCGCGACGgcgcacggtagt                                                                                                                     >  2:168401/1‑243 (MQ=255)                                                                                                         cgccAGCACCGACCGTGCTTTAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCtgcg                                                                                                                <  1:189831/251‑1 (MQ=255)                                                                                                                 ccgaccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAGCATCGGCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGa                                                                                                        <  1:192047/251‑1 (MQ=255)                                                                                                                   gaccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGagc                                                                                                      >  1:219151/1‑249 (MQ=255)                                                                                                                     ccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTAGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTg                                                                                                    >  1:19631/1‑251 (MQ=255)                                                                                                                                                                       gatAAAATTGCCTACATATACGCCAACTGCGGTATCACAAAGAACGAGAATGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGCTAACACCATCGTCTTCACCACCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGCCGCAGGTTATATTTCGGGTATGGAGCTGGTCGACCACAGTATGCCGGAGCTGACGTCTCTTCTTCGCGATgcagacg                                                   >  2:340567/3‑244 (MQ=255)                                                                                                                                                                            aaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCg                                                                                                                           >  1:287743/1‑173 (MQ=255)                                                                                                                                                                            aaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCg                                                                                                                           <  2:287743/173‑1 (MQ=255)                                                                                                                                                                            aaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTg                                              >  1:171088/1‑250 (MQ=255)                                                                                                                                                                            aaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGGTTGACGCCACCATAAAAGAGATGAAAGACGCGGGGGATAACACCATCGTCTTCACCAACTTCGTTGACTTAGACTCTTCCTGGGGCCTCCGTAGCGACGTCGACGTTTATGCAGCGGGTCTGGAGCTGTTCGACCGACGTCTGCCGGAGCTGATGGCTCTGCTGCGCGagtacgccctacggt                                             >  2:4978/1‑237 (MQ=255)                                                                                                                                                                                 cagACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTc                                        >  1:55212/3‑251 (MQ=255)                                                                                                                                                                                          aCGCCAACTGCGGTATCACCAATAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCt                                  <  1:379881/248‑1 (MQ=255)                                                                                                                                                                                          aCGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAATAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGTCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCActgc                                   <  1:362149/247‑4 (MQ=255)                                                                                                                                                                                             ccAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCAc                            >  1:83880/1‑251 (MQ=255)                                                                                                                                                                                             ccAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCAc                            <  1:11820/251‑1 (MQ=255)                                                                                                                                                                                                aCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGGGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTTCTGGCCACGGt                         >  2:109560/1‑251 (MQ=255)                                                                                                                                                                                                   gCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgt                      <  1:142992/251‑2 (MQ=255)                                                                                                                                                                                                   gCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgt                      <  1:13019/251‑2 (MQ=255)                                                                                                                                                                                                     ggTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGCGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGCTGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTgtg                     >  1:22146/1‑248 (MQ=255)                                                                                                                                                                                                       tATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATc                  >  1:322595/1‑251 (MQ=255)                                                                                                                                                                                                                       aaaGCCACTTGCCTGGCAGCGCGGTTTGACGCCAACATCAAAGAGATGAAAGAAGCGGGTGATCACACCACCGTCTTCCCCAACTTCGATGATTTCGACTCTTCCTGGTGCCCCCGTCGCGACGTCTCCGGTTATGCTGCGGGTTTGGAGCTGTTCGACCTCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCTCACTGCTGAACACGGTTGTGATCCGACCTGGACCGGTAc  <  2:313362/251‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTAC  >  NC_000913/4620208‑4620672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑