Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,358	C→T	G252G (GGC→GGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,358	0	C	T	96.7%	86.1 / NA	30	G252G (GGC→GGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/14);  minor base G (0/1);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTG  >  NC_000913/4620116‑4620574                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         gCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAggtggt                                                                                                                                                                                                                  <  1:158973/251‑1 (MQ=255)       tCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGAGGCTACAAAAACGGTCGTGTTATCGCTCGTCAGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGTTGATGTAAAACACGGTCAGGGGGTTTATGt                                                                                                                                                                                                            >  1:22104/1‑251 (MQ=255)        ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTc                                                                                                                                                                                                           <  1:319598/251‑1 (MQ=255)        ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTc                                                                                                                                                                                                           >  1:306294/1‑251 (MQ=255)         cGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCCCCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCg                                                                                                                                                                                                          >  1:313362/1‑251 (MQ=255)                  gATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCAGCTACAACATCTGTCGCGTTATCGCACCTCCGTTTATCGGTGACCAAGCCGGTGACTTCCAGCGTACCGGGAACCGTCACGCCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGGAACTGGTTGATGCAAAACACGGTTAGGTGGGTTCTTGCGGTAAAAttt                                                                                                                                                                                                 >  1:132679/1‑250 (MQ=255)                                aaggaaccttaggccaggATAAACCCTACGAACTGTGCGAAATCGCCCGTGAAGAGCGGACCACCGGCGGCGACAACATCGGTCGGGTTATCGCTCGTCCGTTTAGCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGcc                                                                                                                                                                                   <  2:100362/235‑1 (MQ=255)                                           gtgccggATAAACTCTACGAACTGCTCGAAATCGCTCTTTGAGTGCTGACCCACGGCGGCTACAATTTCGGTCGTGTTATCGCTCGTCCGTTTATGGGCGACAAAGCCGGTAACTCCCATCGTACCGGTAACCGTCATGACCTGGCTGTTGAGCCGCGAGCACCGACCGTGCGGCAGAAGCTGGTTGATGAAAAACACGGGCAGGTGGTTTCTGTCGGGAAAATTGCGGACATCTACGCCAACTGCGGTAt                                                                                                                                                                        <  2:165880/246‑1 (MQ=255)                                            gccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTCAAATTGCAGACATCTACGCCAACTGCGGTATc                                                                                                                                                                       >  1:311224/3‑251 (MQ=255)                                                ggATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCAcc                                                                                                                                                                    >  1:339207/1‑250 (MQ=255)                                                   tAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCGGTAAAATTTCCGACATCTACGCCCACTGCGGTATCAccc                                                                                                                                                                   >  1:159809/1‑247 (MQ=255)                                                     aaccctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaagaaa                                                                                                                                                              <  1:374797/247‑1 (MQ=255)                                                      aCTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAg                                                                                                                                                             <  1:161927/251‑1 (MQ=255)                                                           aCGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGaaa                                                                                                                                                        <  1:76168/251‑1 (MQ=255)                                                                                             cAACGGCGGCTACAACATCTGTCGTGTTATCGCTCGTCCGTTTTTCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGTCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGtcacca                                                                                                                      <  1:69963/251‑1 (MQ=255)                                                                                                    ggcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCaa                                                                                                                  >  1:53314/1‑248 (MQ=255)                                                                                                                       ttATCGCTCGTCCGTTTAGCGGTGACAAAGCCGTTAACTTCCAGCGTACCGGTACCCGCCACGCCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGa                                                                                             <  2:314005/250‑1 (MQ=255)                                                                                                                            gCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTAACGACCTGGCTGTTGAGCCGCCAGCACCGACAGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTATGTCGGTAAAATTGCAGAAATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAGGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGGGATAacac                                                                                       >  1:170132/1‑251 (MQ=255)                                                                                                                               cGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAcc                                                                                      <  1:190136/249‑1 (MQ=255)                                                                                                                                   cgtttaccggtaaCAAAACCGGTACTTTCCCGCGTACCGGTAACCGTCACGACCTTGTTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCTCCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACAATCGTc                                                                                <  2:92808/239‑1 (MQ=255)                                                                                                                                               aaaaaGCCTGTAACTTCCTGGTTACCGGTAACCGCAACGGCCTGGATGGTGAGCCGCCAGTACCGACCGTGCTGCATAAACTGGTTTATGAAAAACACGGTCAGGTGGTGTCTGTCGGTAAAAGTGCAGACATCTACGCCATCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGGCTGTACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATACCACCATAGTCTTCACCAACTTc                                                                    <  2:56004/249‑1 (MQ=255)                                                                                                                                                      cGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACt                                                             >  1:12333/1‑251 (MQ=255)                                                                                                                                                                           tAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCAcc                                        >  1:291890/1‑251 (MQ=255)                                                                                                                                                                              ccGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTc                                     <  1:67843/251‑1 (MQ=255)                                                                                                                                                                                            cTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGCCCACCGTCGCGACGTCGCCgtt                       >  2:11820/1‑249 (MQ=255)                                                                                                                                                                                                tGAGCCGCCAGCAACGACAGTGCTGCAGAAACTGGTTGATGAAAAACACACTCAGGTGGTTTCTGACGGTACAATTGCAGACAACTACGCCAACTGCGGCATCACCACGCACGAGAAAGCAACTGGCCTGGACGCGCTGTTTGCCGCCACCATCAAAGAGATGAAAGACGCGGGTGATAACACCATCGTCTTAACCAAATTCGTTGACTTCGACATTTCCTGGAGCCACCGTCGCGACGgcgcacggtagt                   >  2:168401/1‑243 (MQ=255)                                                                                                                                                                                                     cgccAGCACCGACCGTGCTTTAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCtgcg              <  1:189831/251‑1 (MQ=255)                                                                                                                                                                                                             ccgaccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAGCATCGGCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGa      <  1:192047/251‑1 (MQ=255)                                                                                                                                                                                                               gaccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGagc    >  1:219151/1‑249 (MQ=255)                                                                                                                                                                                                                 ccgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTAGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTg  >  1:19631/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         GCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTG  >  NC_000913/4620116‑4620574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑