Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,150	A→G	E183G (GAA→GGA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,150	0	A	G	90.3%	71.1 / 0.1	31	E183G (GAA→GGA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (18/10);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

GTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAA‑GAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTG  >  NC_000913/4619930‑4620383                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          gTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAACCAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGACCGCGCTAATCTGCCGGGTTACCTCGGTAACTGACACTCTTCCGGTACGGTCATTCTGGATCATCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGCTTGCCTGCCACGAA‑GGAACCTTTGTCCTGGATAAACTCTACGAACt                                                                                                                                                                                                             >  2:151379/1‑251 (MQ=255)       tttGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCg                                                                                                                                                                                                        >  2:158973/1‑250 (MQ=255)         tGAGTGGGGATATTTCTCCGATCACGAAATCAGCTTCCCTCAAGAGCTGATGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGCCAAGCCGATTTTCTATACCTCCGCTGCATCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACttggcaaa                                                                                                                                                                                                      >  2:190136/1‑243 (MQ=255)                                  tcccacaactccccccAAGTGCTGCGGGATAAATTGGTCGAACGCTCTAAGCGGCCGGTTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcg                                                                                                                                                                             <  2:221143/235‑1 (MQ=255)                                            tCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcg                                                                                                                                                                             >  1:221143/1‑240 (MQ=255)                                             cccGAAAGAGCTGCTGGATAAACTGGTCGACCGCCCTAATCTGCCGGGTTCCTTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaaca                                                                                                                                                                 <  1:304194/251‑3 (MQ=255)                                                         gctgGATAAACTGGTCGAACGCTCTAATCTGCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTa                                                                                                                                                     <  1:179060/251‑1 (MQ=255)                                                            ggATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAA‑GAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATcc                                                                                                                                                  >  1:132260/1‑250 (MQ=255)                                                             gATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCCGGCGTGTTATCGc                                                                                                                                                 >  1:101726/1‑251 (MQ=255)                                                                aaaCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCCGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCCCGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGACGGCTACACAATCGGTCGGGTTATTGCTCg                                                                                                                                              >  2:76168/1‑251 (MQ=255)                                                                      gTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTGCAACATCGGtagggtttcctcccggtcgtt                                                                                                                                        >  2:143519/1‑231 (MQ=255)                                                                         aaccgcctaaattgtcggggttctcTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGAACAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAA‑GAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTAt                                                                                                                                     <  2:132260/227‑1 (MQ=255)                                                                                       gCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCg                                                                                                                       <  1:143519/251‑1 (MQ=255)                                                                                                 cTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGGCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTcc                                                                                                              >  1:314005/1‑250 (MQ=255)                                                                                                                           ggTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTgg                                                                                   >  1:92808/1‑251 (MQ=255)                                                                                                                           ggTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTAGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCCGCGTCCCGGGAACCGTCACGACCTgg                                                                                   >  1:285591/1‑251 (MQ=255)                                                                                                                            gTCATTCTGGAGCAGCTGTGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGACCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGCAGAGCTGACCAACGGCGGCTACACCATCGGTCGTGTTATCGCTAGGCCGTTTATCGGTGACCAAGCACGTAACTTCCAGCGTAACGGTAACCGTCaagcaccggc                                                                                  >  2:67843/1‑242 (MQ=255)                                                                                                                                                 gAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTAGCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCaccgac                                                             <  1:333415/251‑1 (MQ=255)                                                                                                                                                        acaTGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTTAGCCGCCAGCCCCGACCGtgctg                                                      >  1:350041/1‑251 (MQ=255)                                                                                                                                                               aaCCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCCACAACATCGGTCGTGGTATCGCCCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTCACGGTAACCGTCACGACCTGGCTGTTGGGCCACCAGCACCAGCCGGGCTGCaaaacc                                               >  1:98013/1‑246 (MQ=255)                                                                                                                                                                  cGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGATCTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTgg                                            <  1:350669/251‑1 (MQ=255)                                                                                                                                                                   ggCAAGCCTATTTTCTATCCCTCCGCTGTCTCCGTGTTCCAGATTGCCTGCCACGAATGATCCTTT‑GGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACATCACCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCTGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGt                                           <  1:256993/251‑1 (MQ=255)                                                                                                                                                                              tttCTATACCTCCGCTGCCTCCGTGTACCAGATTGCCTGCCACGAC‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCCGAAACTGGTTGATGAAAAac                                >  1:303974/1‑251 (MQ=255)                                                                                                                                                                               ttCTATACCTCCGCTGACTCCGTGTTCAAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAac                                <  1:151379/250‑1 (MQ=255)                                                                                                                                                                                   atcccTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCGCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAacac                              <  1:360013/245‑1 (MQ=255)                                                                                                                                                                                           gCTGACTCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAggtggt                   <  1:158973/251‑1 (MQ=255)                                                                                                                                                                                                 tCCGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGAGGCTACAAAAACGGTCGTGTTATCGCTCGTCAGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGTTGATGTAAAACACGGTCAGGGGGTTTATGt             >  1:22104/1‑251 (MQ=255)                                                                                                                                                                                                  ccGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTc            <  1:319598/251‑1 (MQ=255)                                                                                                                                                                                                  ccGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTc            >  1:306294/1‑251 (MQ=255)                                                                                                                                                                                                   cGTGTTCCAGATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCCCCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCg           >  1:313362/1‑251 (MQ=255)                                                                                                                                                                                                            gATTGCCTGCCACGAA‑GGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCAGCTACAACATCTGTCGCGTTATCGCACCTCCGTTTATCGGTGACCAAGCCGGTGACTTCCAGCGTACCGGGAACCGTCACGCCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGGAACTGGTTGATGCAAAACACGGTTAGGTGGGTTCTTGCGGTAAAAttt  >  1:132679/1‑250 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          GTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAA‑GAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTG  >  NC_000913/4619930‑4620383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑