Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,067	A→G	Q155Q (CAA→CAG)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,067	0	A	G	87.0%	51.0 / 0.7	24	Q155Q (CAA→CAG)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (14/6);  total (17/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

GGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGC  >  NC_000913/4619821‑4620303                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             ggcggGAAGGTACGGAAACGCTGAGGTTATCGGGTCGTACGCTTGGGCGCACGAAATGTCAGCCGGTAAAGAACCCCCGTCTGGTCCCTGGGAAACCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAACCGGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTgg                                                                                                                                                                                                                                          <  2:142387/251‑1 (MQ=255)      gAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGa                                                                                                                                                                                                                                      >  1:293933/1‑250 (MQ=255)           gACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGca                                                                                                                                                                                                                                <  1:239312/251‑1 (MQ=255)                          gTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGTTCAGCTGGGCGAAGAGCACATGAAAACCGGCa                                                                                                                                                                                                                  >  1:56004/1‑250 (MQ=255)                                            tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTc                                                                                                                                                                                               >  1:165880/1‑251 (MQ=255)                                                cgcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACGGGGAAATCGCAGGCGTACCGGTTCTGTTTGAGAGGGGATATTTCTCCGATCACGAAAACAGATTCCCGCAAGAGCTGATGGATAAACTGGTCGAACGCGCTAATCTTCCACGTTACCTAGGTCACTGCCTCACTTCCTGAACGGTCCTTCTGGATCAGCTGGGCGCACATCACATGAACACCGGCCAGCCGATTTTCtataaccccgcc                                                                                                                                                                                           >  2:374797/1‑243 (MQ=255)                                                                    aaaGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGCGTGGGGATATTTATCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAAATGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgc                                                                                                                                                                       >  2:304194/1‑251 (MQ=255)                                                                                                              gTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAACCAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGACCGCGCTAATCTGCCGGGTTACCTCGGTAACTGACACTCTTCCGGTACGGTCATTCTGGATCATCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGCTTGCCTGCCACGAAGGAACCTTTGTCCTGGATAAACTCTACGAACt                                                                                                                             >  2:151379/1‑251 (MQ=255)                                                                                                                    tttGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCg                                                                                                                        >  2:158973/1‑250 (MQ=255)                                                                                                                      tGAGTGGGGATATTTCTCCGATCACGAAATCAGCTTCCCTCAAGAGCTGATGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGCCAAGCCGATTTTCTATACCTCCGCTGCATCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACttggcaaa                                                                                                                      >  2:190136/1‑243 (MQ=255)                                                                                                                                               tcccacaactccccccAAGTGCTGCGGGATAAATTGGTCGAACGCTCTAAGCGGCCGGTTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcg                                                                                             <  2:221143/235‑1 (MQ=255)                                                                                                                                                         tCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAAcg                                                                                             >  1:221143/1‑240 (MQ=255)                                                                                                                                                          cccGAAAGAGCTGCTGGATAAACTGGTCGACCGCCCTAATCTGCCGGGTTCCTTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaaca                                                                                 <  1:304194/251‑3 (MQ=255)                                                                                                                                                                      gctgGATAAACTGGTCGAACGCTCTAATCTGCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTa                                                                     <  1:179060/251‑1 (MQ=255)                                                                                                                                                                         ggATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATcc                                                                  >  1:132260/1‑250 (MQ=255)                                                                                                                                                                          gATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCCGGCGTGTTATCGc                                                                 >  1:101726/1‑251 (MQ=255)                                                                                                                                                                             aaaCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCCGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCCCGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGACGGCTACACAATCGGTCGGGTTATTGCTCg                                                              >  2:76168/1‑251 (MQ=255)                                                                                                                                                                                   gTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTGCAACATCGGtagggtttcctcccggtcgtt                                                        >  2:143519/1‑231 (MQ=255)                                                                                                                                                                                      aaccgcctaaattgtcggggttctcTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGAACAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTAt                                                     <  2:132260/227‑1 (MQ=255)                                                                                                                                                                                                    gCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCg                                       <  1:143519/251‑1 (MQ=255)                                                                                                                                                                                                              cTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGGCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTcc                              >  1:314005/1‑250 (MQ=255)                                                                                                                                                                                                                                        ggTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTgg   >  1:92808/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTAGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCCGCGTCCCGGGAACCGTCACGACCTgg   >  1:285591/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTCATTCTGGAGCAGCTGTGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGACCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGCAGAGCTGACCAACGGCGGCTACACCATCGGTCGTGTTATCGCTAGGCCGTTTATCGGTGACCAAGCACGTAACTTCCAGCGTAACGGTAACCGTCaagcaccggc  >  2:67843/1‑242 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGC  >  NC_000913/4619821‑4620303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑