Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,454	A→G	N409S (AAC→AGC)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,454	0	A	G	89.2%	89.3 / ‑1.0	37	N409S (AAC→AGC)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (6/27);  total (8/29)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

TGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGAC  >  NC_000913/4619229‑4619689                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            tGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGGTTTTCC‑CGCGGAAGACGAAAAAAGCTGGCCGGGAGCGGCGAAAGCgggg                                                                                                                                                                                                                    >  1:47395/1‑249 (MQ=255)    cGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGaaa                                                                                                                                                                                                                 <  1:59220/251‑1 (MQ=255)    cGACAGCGATGCTGACGAAAGCAGACTATGCTGATACCGAGGGTTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCACTGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTGCTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTGTCCCCGCTGAAGAGGAAAACAGCTGGCCGGAAgcggcggaaaaggtgaa                                                                                                                                                                                                                  >  1:20843/1‑239 (MQ=255)      aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGc                                                                                                                                                                                                               <  1:112755/251‑1 (MQ=255)        aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGAAGACGGTCAGCGTCCGCTGGCGG‑GTATTCACGAGAAAAAAGAAAGAAACTGACAGGAAGCGGCGAAAGAGGTGGAAgggg                                                                                                                                                                                                             >  1:58610/1‑248 (MQ=255)                 gACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTaa                                                                                                                                                                                                      <  1:290908/249‑1 (MQ=255)                                 gCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCAc                                                                                                                                                                                     <  1:53974/250‑1 (MQ=255)                                    gATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCg                                                                                                                                                                                   <  1:382663/249‑1 (MQ=255)                                           aaGGTTGTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGcaca                                                                                                                                                                          <  2:47395/251‑1 (MQ=255)                                                             tGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTTTCCACGCGAAAGACGGAAACAGCTGGCAGGAAGGGGCGAAAGCGGGGAAAGAGGCAATTAAACTTTCCGATAAAGCACCCGGAAGCACCCCCACTGGCTATCGCCgg                                                                                                                                                        >  1:56314/1‑250 (MQ=255)                                                               gATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTAt                                                                                                                                                      <  1:212704/251‑1 (MQ=255)                                                                      gcgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGTTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGaa                                                                                                                                               <  1:196411/251‑1 (MQ=255)                                                                       cgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACt                                                                                                                                                                                                   <  1:123870/198‑1 (MQ=255)                                                                          gcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAAc                                                                                                                                           <  1:123634/251‑1 (MQ=255)                                                                                          gTTGCAATGGGCTGCGGTCGTCGTCAGTCATCTGACACCATTGATTACCGCGTCTGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATACACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGTAAAGCACACCAACTGTCTATCGTCGTATCAGCGAATAACGGTATActgatctgat                                                                                                                           <  1:140176/251‑1 (MQ=255)                                                                                             gaaatgggaggcggttgtcGTCATGCATATGAAAACATTGATTACAGCGGCGGCTTTAATGATATGGCGCGTTTGGGCGAAAAGGTAGACGGTCAGCGGCCGCTGGCGG‑TTATCCACGCGAAAGAAGAAAACAGATGGAAGGAAGCGGCGAAAGCGGTGAAAGCGGAAATTAAACTTGCCGATAAAGCAACGGAAAGCACACCAACTGTATATCGCAGTATCAGCGAATAACGGTATActgatctgataa                                                                                                                         <  2:81265/232‑3 (MQ=255)                                                                                                gtGGGCGGCGGTCTTCGTCAGGCAACTGAACCCATTGATTACGGCGTCGGCTTTACTGATATGGCGCGTTGGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTa                                                                                                                     <  2:56314/250‑1 (MQ=255)                                                                                                                          tGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCCCGCGGAAAACGAAAACAGCTGGCCGGACGCGGCGGAAGCGGTGAAAGCGGCGATTAAACTTTACGATAAAGCACAGGGAAGCACACCAACTGTCTATCGGCGTATCAGCGAATAAAGGCATACTTATCTTAACATTTAAATTTGAAGCaattggtacggcgaac                                                                                           >  1:52586/1‑236 (MQ=255)                                                                                                                           gACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGaa                                                                                            <  1:277255/249‑1 (MQ=255)                                                                                                                           gACACCATTGATGACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGTCGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGTGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACa                                                                                          <  1:353682/251‑1 (MQ=255)                                                                                                                             cacCATTGATTACAGCCTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACata                                                                                        <  1:218653/251‑1 (MQ=255)                                                                                                                                   tgATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGaaa                                                                                   <  1:241580/249‑1 (MQ=255)                                                                                                                                           gCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCAttt                                                                          <  1:208679/251‑1 (MQ=255)                                                                                                                                                ggCTTTACGGATATGGCGCGTGTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTtattat                                                                     <  1:46040/251‑1 (MQ=255)                                                                                                                                                    ttACTGATATGTCGGGTGTGGGCGACCAGGTAGCCGTGCAGCGTCCGCTTGCGG‑TTACCCACGCGAAAGACGAAAACAGCTGTCAGGAATCGGCGAAAGCTGTTACAGCTGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTATATCGCCGTATCAGCTAATAACGGTATACTGATCTGATCAGTTAAATTTGAAGCACTGAGTACGGAGATCATATTAATCGTGCTTTtattattgt                                                                  <  2:227065/250‑4 (MQ=255)                                                                                                                                                      ccggATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTAGCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCTGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCt                                                               <  2:142576/248‑1 (MQ=255)                                                                                                                                                         gATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTgg                                                             <  1:278194/250‑1 (MQ=255)                                                                                                                                                                 gcgTTTGGGCGACCAGGTAGACGGTGAGCGTCCGCTGGCTG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTTTCTATCGCTGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCg                                                    <  2:244688/251‑1 (MQ=255)                                                                                                                                                                         gtgACCAGGGTGACGGTCAGCTTCCGCTGGCTG‑TTATCCACGCGAAAGACGAAAATAACTGGCAGGAAGCGGCGAATGCGGTGAAAGTGGTAATTAAACTTGCCGATAAATCACCGGAAAGCACACTAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTTATCATTTAAAGTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTGATTATGGTGCTGGACTCATTCGGCATCggt                                            <  2:254916/249‑2 (MQ=255)                                                                                                                                                                           gACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGgcgc                                          <  1:42814/251‑1 (MQ=255)                                                                                                                                                                              cAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAAAAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGGAAGCACACCCACCGTCTATTGCCGTATCAGCGAATAACGGTATACTGCTCTGATCATTTAAATTTTAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGTCTCATTCGGCATCGGCGCTAc                                       >  1:184/1‑251 (MQ=255)                                                                                                                                                                                  gaGACGGTCAGCGGCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACagaa                                   <  1:28616/250‑1 (MQ=255)                                                                                                                                                                                    gactgTCAGCGTCCGCGGGCGG‑TTATCCACGCGAAAGACGGAAACAACTGGCAGGAAGCAGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACagaaga                                 <  2:187785/247‑1 (MQ=255)                                                                                                                                                                                    gACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGGAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACagaa                                   >  1:90442/1‑249 (MQ=255)                                                                                                                                                                                    gACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACagaa                                   <  2:90442/249‑1 (MQ=255)                                                                                                                                                                                           ggCGTCCGCTGGCTG‑TAATCACCGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAATCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTTATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACATAAGATGCAGaa                          <  2:49485/250‑1 (MQ=255)                                                                                                                                                                                                                    cgtaaGACGAAAACCGCTGGCAGGAAGCGGCGAAAGCGGGGACAGCGGCAATTCAACTTGCCGCTCCAGCACAGGCAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGACGCACTATGTACGGAGGACATGTGAAACGTGCATTTATTATGGTGCTGTACTCATTACGCATCGGCGCTACAGAAGATGCCGCACGCCTTGGTGCCGTCGGGGCtaac  >  2:179060/4‑248 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            TGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGAC  >  NC_000913/4619229‑4619689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑