Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,393	C→T	L389L (CTG→TTG)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,393	0	C	T	93.9%	80.8 / NA	35	L389L (CTG→TTG)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (8/23);  minor base G (0/2);  total (9/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCG  >  NC_000913/4619159‑4619639                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        gaagtagttgggcgttgtgTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCGGGGct                                                                                                                                                                                                                                                   <  2:105839/223‑2 (MQ=255) gAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCg                                                                                                                                                                                                                                                   >  1:105839/1‑241 (MQ=255)  aattCTTTGGTGGTATGGTAGCGTCACAAAAAGGCCCAACTGACTTCGTTTAGAACTACTCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATTCCGAAGGTTTTTTCAGTGAAATGGATACCGGCGCGCTGGGGATGGCAGTGGTTGCGATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGAc                                                                                                                                                                                                                                        <  2:158906/248‑1 (MQ=255)  aaGTCTTTGGTCGTAAGGTAGCGGCACAAAAAGGCACAACCGAATTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGCTGCTGACGAAAGCAGTCTATGCTGATACCGAAGGGTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTAGGCAGGCTTCTGCCACCCTTGATTACCGCGTAGGATTTACTTGTAAGGCGCGTTTggggtccaagggagac                                                                                                                                                                                                                                        >  2:290908/1‑238 (MQ=255)                aTGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACACGCGCGCTGGGGATGGCAGAGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGCCACCATTGATTACAGCGTCGGCTTGACTGATATGGCCCGTTTGGGCGACCAGGTAGACGGACAGTGTcctct                                                                                                                                                                                                                          >  2:208679/1‑248 (MQ=255)                         gCACAAAAAGGCCCAACCGACTTCGTTGAGAACTTCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCTCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTTGCGG‑TTAt                                                                                                                                                                                                                <  1:213166/251‑1 (MQ=255)                                aaGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCAcgcg                                                                                                                                                                                                         >  1:49485/1‑251 (MQ=255)                                                                       tGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGGTTTTCC‑CGCGGAAGACGAAAAAAGCTGGCCGGGAGCGGCGAAAGCgggg                                                                                                                                                                  >  1:47395/1‑249 (MQ=255)                                                                          cGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGaaa                                                                                                                                                               <  1:59220/251‑1 (MQ=255)                                                                          cGACAGCGATGCTGACGAAAGCAGACTATGCTGATACCGAGGGTTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCACTGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTGCTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTGTCCCCGCTGAAGAGGAAAACAGCTGGCCGGAAgcggcggaaaaggtgaa                                                                                                                                                                >  1:20843/1‑239 (MQ=255)                                                                            aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGc                                                                                                                                                             <  1:112755/251‑1 (MQ=255)                                                                              aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGAAGACGGTCAGCGTCCGCTGGCGG‑GTATTCACGAGAAAAAAGAAAGAAACTGACAGGAAGCGGCGAAAGAGGTGGAAgggg                                                                                                                                                           >  1:58610/1‑248 (MQ=255)                                                                                       gACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTaa                                                                                                                                                    <  1:290908/249‑1 (MQ=255)                                                                                                       gCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCAc                                                                                                                                   <  1:53974/250‑1 (MQ=255)                                                                                                          gATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCg                                                                                                                                 <  1:382663/249‑1 (MQ=255)                                                                                                                 aaGGTTGTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGcaca                                                                                                                        <  2:47395/251‑1 (MQ=255)                                                                                                                                   tGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTTTCCACGCGAAAGACGGAAACAGCTGGCAGGAAGGGGCGAAAGCGGGGAAAGAGGCAATTAAACTTTCCGATAAAGCACCCGGAAGCACCCCCACTGGCTATCGCCgg                                                                                                      >  1:56314/1‑250 (MQ=255)                                                                                                                                     gATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTAt                                                                                                    <  1:212704/251‑1 (MQ=255)                                                                                                                                            gcgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGTTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGaa                                                                                             <  1:196411/251‑1 (MQ=255)                                                                                                                                             cgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACt                                                                                                                                                 <  1:123870/198‑1 (MQ=255)                                                                                                                                                gcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAAc                                                                                         <  1:123634/251‑1 (MQ=255)                                                                                                                                                                gTTGCAATGGGCTGCGGTCGTCGTCAGTCATCTGACACCATTGATTACCGCGTCTGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATACACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGTAAAGCACACCAACTGTCTATCGTCGTATCAGCGAATAACGGTATActgatctgat                                                                         <  1:140176/251‑1 (MQ=255)                                                                                                                                                                   gaaatgggaggcggttgtcGTCATGCATATGAAAACATTGATTACAGCGGCGGCTTTAATGATATGGCGCGTTTGGGCGAAAAGGTAGACGGTCAGCGGCCGCTGGCGG‑TTATCCACGCGAAAGAAGAAAACAGATGGAAGGAAGCGGCGAAAGCGGTGAAAGCGGAAATTAAACTTGCCGATAAAGCAACGGAAAGCACACCAACTGTATATCGCAGTATCAGCGAATAACGGTATActgatctgataa                                                                       <  2:81265/232‑3 (MQ=255)                                                                                                                                                                      gtGGGCGGCGGTCTTCGTCAGGCAACTGAACCCATTGATTACGGCGTCGGCTTTACTGATATGGCGCGTTGGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTa                                                                   <  2:56314/250‑1 (MQ=255)                                                                                                                                                                                                tGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCCCGCGGAAAACGAAAACAGCTGGCCGGACGCGGCGGAAGCGGTGAAAGCGGCGATTAAACTTTACGATAAAGCACAGGGAAGCACACCAACTGTCTATCGGCGTATCAGCGAATAAAGGCATACTTATCTTAACATTTAAATTTGAAGCaattggtacggcgaac                                         >  1:52586/1‑236 (MQ=255)                                                                                                                                                                                                 gACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGaa                                          <  1:277255/249‑1 (MQ=255)                                                                                                                                                                                                 gACACCATTGATGACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGTCGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGTGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACa                                        <  1:353682/251‑1 (MQ=255)                                                                                                                                                                                                   cacCATTGATTACAGCCTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACata                                      <  1:218653/251‑1 (MQ=255)                                                                                                                                                                                                         tgATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGaaa                                 <  1:241580/249‑1 (MQ=255)                                                                                                                                                                                                                 gCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCAttt                        <  1:208679/251‑1 (MQ=255)                                                                                                                                                                                                                      ggCTTTACGGATATGGCGCGTGTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTtattat                   <  1:46040/251‑1 (MQ=255)                                                                                                                                                                                                                          ttACTGATATGTCGGGTGTGGGCGACCAGGTAGCCGTGCAGCGTCCGCTTGCGG‑TTACCCACGCGAAAGACGAAAACAGCTGTCAGGAATCGGCGAAAGCTGTTACAGCTGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTATATCGCCGTATCAGCTAATAACGGTATACTGATCTGATCAGTTAAATTTGAAGCACTGAGTACGGAGATCATATTAATCGTGCTTTtattattgt                <  2:227065/250‑4 (MQ=255)                                                                                                                                                                                                                            ccggATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTAGCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCTGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCt             <  2:142576/248‑1 (MQ=255)                                                                                                                                                                                                                               gATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTgg           <  1:278194/250‑1 (MQ=255)                                                                                                                                                                                                                                       gcgTTTGGGCGACCAGGTAGACGGTGAGCGTCCGCTGGCTG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTTTCTATCGCTGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCg  <  2:244688/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        GAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCG  >  NC_000913/4619159‑4619639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑