Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,359	C→T	I377I (ATC→ATT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,359	0	C	T	91.4%	83.3 / ‑0.8	35	I377I (ATC→ATT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (10/22);  total (12/23)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

AAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATA  >  NC_000913/4619112‑4619603                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aaGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGGAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACCAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGAt                                                                                                                                                                                                                                                    >  1:187785/1‑251 (MQ=255)              aTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTAGCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCt                                                                                                                                                                                                                                       <  2:122036/251‑1 (MQ=255)                     gcagTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGa                                                                                                                                                                                                                                <  1:138132/248‑1 (MQ=255)                          gCTGGAAAACGATAAAGCGGCAAAAGTCTTTGCTCGTATGGTAGCGGCACCAAAAGGCCAAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAACGATGCTGACGAAAGCAGTCTATGCTGATACCGACGGTTTTGTCAGTGAAATGGATACCAGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCTGTCAGCGCAAGGCATCTGACACCATTGATTACATCGTCGGCCTTaatgttatcg                                                                                                                                                                                                                           >  2:241580/1‑242 (MQ=255)                             ggACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGgcgc                                                                                                                                                                                                                        <  1:84403/251‑1 (MQ=255)                                                gaagtagttgggcgttgtgTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCGGGGct                                                                                                                                                                                                               <  2:105839/223‑2 (MQ=255)                                                gAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCg                                                                                                                                                                                                               >  1:105839/1‑241 (MQ=255)                                                 aattCTTTGGTGGTATGGTAGCGTCACAAAAAGGCCCAACTGACTTCGTTTAGAACTACTCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATTCCGAAGGTTTTTTCAGTGAAATGGATACCGGCGCGCTGGGGATGGCAGTGGTTGCGATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGAc                                                                                                                                                                                                    <  2:158906/248‑1 (MQ=255)                                                 aaGTCTTTGGTCGTAAGGTAGCGGCACAAAAAGGCACAACCGAATTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGCTGCTGACGAAAGCAGTCTATGCTGATACCGAAGGGTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTAGGCAGGCTTCTGCCACCCTTGATTACCGCGTAGGATTTACTTGTAAGGCGCGTTTggggtccaagggagac                                                                                                                                                                                                    >  2:290908/1‑238 (MQ=255)                                                               aTGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACACGCGCGCTGGGGATGGCAGAGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGCCACCATTGATTACAGCGTCGGCTTGACTGATATGGCCCGTTTGGGCGACCAGGTAGACGGACAGTGTcctct                                                                                                                                                                                      >  2:208679/1‑248 (MQ=255)                                                                        gCACAAAAAGGCCCAACCGACTTCGTTGAGAACTTCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCTCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTTGCGG‑TTAt                                                                                                                                                                            <  1:213166/251‑1 (MQ=255)                                                                               aaGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCAcgcg                                                                                                                                                                     >  1:49485/1‑251 (MQ=255)                                                                                                                      tGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGGTTTTCC‑CGCGGAAGACGAAAAAAGCTGGCCGGGAGCGGCGAAAGCgggg                                                                                                                              >  1:47395/1‑249 (MQ=255)                                                                                                                         cGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGaaa                                                                                                                           <  1:59220/251‑1 (MQ=255)                                                                                                                         cGACAGCGATGCTGACGAAAGCAGACTATGCTGATACCGAGGGTTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCACTGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTGCTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTGTCCCCGCTGAAGAGGAAAACAGCTGGCCGGAAgcggcggaaaaggtgaa                                                                                                                            >  1:20843/1‑239 (MQ=255)                                                                                                                           aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGc                                                                                                                         <  1:112755/251‑1 (MQ=255)                                                                                                                             aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGAAGACGGTCAGCGTCCGCTGGCGG‑GTATTCACGAGAAAAAAGAAAGAAACTGACAGGAAGCGGCGAAAGAGGTGGAAgggg                                                                                                                       >  1:58610/1‑248 (MQ=255)                                                                                                                                      gACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTaa                                                                                                                <  1:290908/249‑1 (MQ=255)                                                                                                                                                      gCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCAc                                                                                               <  1:53974/250‑1 (MQ=255)                                                                                                                                                        tGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATTGCAGTGGTTGCAAGGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCtt                                                                                                                                                                                                                                      <  2:218963/114‑1 (MQ=255)                                                                                                                                                        tGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCtt                                                                                                                                                                                                                                      >  1:218963/1‑114 (MQ=255)                                                                                                                                                         gATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCg                                                                                             <  1:382663/249‑1 (MQ=255)                                                                                                                                                                aaGGTTGTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGcaca                                                                                    <  2:47395/251‑1 (MQ=255)                                                                                                                                                                                  tGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTTTCCACGCGAAAGACGGAAACAGCTGGCAGGAAGGGGCGAAAGCGGGGAAAGAGGCAATTAAACTTTCCGATAAAGCACCCGGAAGCACCCCCACTGGCTATCGCCgg                                                                  >  1:56314/1‑250 (MQ=255)                                                                                                                                                                                    gATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTAt                                                                <  1:212704/251‑1 (MQ=255)                                                                                                                                                                                           gcgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGTTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGaa                                                         <  1:196411/251‑1 (MQ=255)                                                                                                                                                                                            cgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACt                                                                                                             <  1:123870/198‑1 (MQ=255)                                                                                                                                                                                               gcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAAc                                                     <  1:123634/251‑1 (MQ=255)                                                                                                                                                                                                               gTTGCAATGGGCTGCGGTCGTCGTCAGTCATCTGACACCATTGATTACCGCGTCTGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATACACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGTAAAGCACACCAACTGTCTATCGTCGTATCAGCGAATAACGGTATActgatctgat                                     <  1:140176/251‑1 (MQ=255)                                                                                                                                                                                                                  gaaatgggaggcggttgtcGTCATGCATATGAAAACATTGATTACAGCGGCGGCTTTAATGATATGGCGCGTTTGGGCGAAAAGGTAGACGGTCAGCGGCCGCTGGCGG‑TTATCCACGCGAAAGAAGAAAACAGATGGAAGGAAGCGGCGAAAGCGGTGAAAGCGGAAATTAAACTTGCCGATAAAGCAACGGAAAGCACACCAACTGTATATCGCAGTATCAGCGAATAACGGTATActgatctgataa                                   <  2:81265/232‑3 (MQ=255)                                                                                                                                                                                                                     gtGGGCGGCGGTCTTCGTCAGGCAACTGAACCCATTGATTACGGCGTCGGCTTTACTGATATGGCGCGTTGGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTa                               <  2:56314/250‑1 (MQ=255)                                                                                                                                                                                                                                               tGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCCCGCGGAAAACGAAAACAGCTGGCCGGACGCGGCGGAAGCGGTGAAAGCGGCGATTAAACTTTACGATAAAGCACAGGGAAGCACACCAACTGTCTATCGGCGTATCAGCGAATAAAGGCATACTTATCTTAACATTTAAATTTGAAGCaattggtacggcgaac     >  1:52586/1‑236 (MQ=255)                                                                                                                                                                                                                                                gACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGaa      <  1:277255/249‑1 (MQ=255)                                                                                                                                                                                                                                                gACACCATTGATGACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGTCGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGTGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACa    <  1:353682/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cacCATTGATTACAGCCTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACata  <  1:218653/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      AAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGG‑TTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATA  >  NC_000913/4619112‑4619603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑