Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,197	G→A	P323P (CCG→CCA)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,197	0	G	A	85.7%	73.7 / 1.3	36	P323P (CCG→CCA)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (13/17);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

TGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG  >  NC_000913/4618953‑4619440                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    agAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGACGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCcaaccgac                                                                                                                                                                                                                                               <  1:323068/250‑1 (MQ=255)                    cAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTAGACGTATGGTAGCGGCACAAAAAGGCCCAACAGACTTCGTTGAGAACTAcgcg                                                                                                                                                                                                                             >  1:10913/1‑250 (MQ=255)                           ggTAACGCGTTTGAAGTTCGTGAATCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCt                                                                                                                                                                                                                     <  1:305478/251‑1 (MQ=255)                            gTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTg                                                                                                                                                                                                                    >  1:244688/1‑251 (MQ=255)                                                  aGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAg                                                                                                                                                                                              <  1:294395/251‑1 (MQ=255)                                                        gCAGTTCCTGGCGGGTGTGTATCGTAACCCGCGTCTGTTTTATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGTTCGTATGGTAGCGTCACAAAAATGCCCAACCGTCTTCCTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCt                                                                                                                                                                                        <  1:190678/251‑1 (MQ=255)                                                           gTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATg                                                                                                                                                                                     >  1:227065/1‑251 (MQ=255)                                                             tCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCt                                                                                                                                                                                   >  1:81265/1‑251 (MQ=255)                                                                  actggtgagtaccttAACCCGTGTCTTGTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAc                                                                                                                                                                              <  2:4183/237‑1 (MQ=255)                                                                            atCGGAACCCGCGTCTGTTTGATGTCACGAGGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAAGTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCCCAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGttt                                                                                                                                                                     <  2:196819/250‑1 (MQ=255)                                                                                    ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGa                                                                                                                                                            <  1:355008/251‑1 (MQ=255)                                                                                        gTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGCTGCTGACGAAAGCAGCCTATGCTGATACCGAAGGTTTTGTCAGTGAAATg                                                                                                                                                        >  1:373975/1‑251 (MQ=255)                                                                                                    tCACGATGGCGCTGTGCGTGGAGATTCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGAACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCcgcgcg                                                                                                                                            >  1:182770/1‑251 (MQ=255)                                                                                                                 gttagtggggatgtttctcgtcGGCAAACTGGCTAAAGATGACGCCCAAGCGCGCGCGAAAGTGAAAGCAGTTCTGGACAACTGTAAAGCGTCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTGCTTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTAGGCTGATTCCGAAGGTTTTGTCAGCGAAATGGCTACCCGCGCGATGGGGATggtag                                                                                                                               <  2:312900/230‑4 (MQ=255)                                                                                                                    cGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTgg                                                                                                                            <  1:26669/251‑1 (MQ=255)                                                                                                                       ggAGATGCTTAGCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTGTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTGTTGTCTGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg                                                                                                                         <  2:337035/251‑1 (MQ=255)                                                                                                                             gcttaTCTCCGGCAAACTGGCGAAAGATGACGCCGTAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATgg                                                                                                                   <  2:303845/247‑1 (MQ=255)                                                                                                                              cTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGGATGGGAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCCGGGGATGGCCGGGGTTGCAATggg                                                                                                                  >  1:254916/1‑251 (MQ=255)                                                                                                                                taTCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGggcg                                                                                                                >  1:122036/2‑251 (MQ=255)                                                                                                                                       ggCAAACTGGCGAAAGATTACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTGTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGcggcggtcg                                                                                                         <  2:35575/251‑4 (MQ=255)                                                                                                                                          aaaCTTGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAATCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGTGATGGCAGTGGTTGCAATGGGcggcggtcgtcg                                                                                                      <  1:327362/251‑7 (MQ=255)                                                                                                                                                gCGAAAGACTACGCCGAAGGACGCGCGAAATGTCAAGCGGTGCTGGACAAAGGTAAAGCGACAGTAGTCTTGCGTCGTTTGGTAGCGGCACAAAAAGGCCCAACTGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGTTGCTGACGTAAGCAGTCTTTTCGGATTCCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGTGATGGCAGTGGGTGCAATGGGcggcggtcttattcagga                                                                                                <  2:276909/251‑13 (MQ=255)                                                                                                                                                           cGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACACGCGCGATGGGGATGGCAGAGGTTGCAATGGGCGGCGGTCGTCGTCAGGAATCTGACAcc                                                                                      >  1:124274/1‑250 (MQ=255)                                                                                                                                                              cGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGAAAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAACGGCCCCACCGACTTCGTTGAGAACTACGCGAAGGATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTAAGTGAAATGGATAACCGCGCGCTGGGGATGACAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGAATCTGAAACCAttg                                                                                  >  2:218653/1‑249 (MQ=255)                                                                                                                                                                aaGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGGAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACCAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGAt                                                                                >  1:187785/1‑251 (MQ=255)                                                                                                                                                                         cgAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAACGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGGGGTCGTCGTCAGGCAtatggaaccattggttccagcgtc                                                                       >  2:277255/1‑228 (MQ=255)                                                                                                                                                                             aTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTAGCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCt                                                                   <  2:122036/251‑1 (MQ=255)                                                                                                                                                                                    gcagTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGa                                                            <  1:138132/248‑1 (MQ=255)                                                                                                                                                                                         gCTGGAAAACGATAAAGCGGCAAAAGTCTTTGCTCGTATGGTAGCGGCACCAAAAGGCCAAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAACGATGCTGACGAAAGCAGTCTATGCTGATACCGACGGTTTTGTCAGTGAAATGGATACCAGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCTGTCAGCGCAAGGCATCTGACACCATTGATTACATCGTCGGCCTTaatgttatcg                                                       >  2:241580/1‑242 (MQ=255)                                                                                                                                                                                            ggACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGgcgc                                                    <  1:84403/251‑1 (MQ=255)                                                                                                                                                                                                               gaagtagttgggcgttgtgTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCGGGGct                                           <  2:105839/223‑2 (MQ=255)                                                                                                                                                                                                               gAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCg                                           >  1:105839/1‑241 (MQ=255)                                                                                                                                                                                                                aattCTTTGGTGGTATGGTAGCGTCACAAAAAGGCCCAACTGACTTCGTTTAGAACTACTCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATTCCGAAGGTTTTTTCAGTGAAATGGATACCGGCGCGCTGGGGATGGCAGTGGTTGCGATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCGTTACTGATATGGCGCGTTTGGGCGACCAGGTAGAc                                <  2:158906/248‑1 (MQ=255)                                                                                                                                                                                                                aaGTCTTTGGTCGTAAGGTAGCGGCACAAAAAGGCACAACCGAATTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGCTGCTGACGAAAGCAGTCTATGCTGATACCGAAGGGTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTAGGCAGGCTTCTGCCACCCTTGATTACCGCGTAGGATTTACTTGTAAGGCGCGTTTggggtccaagggagac                                >  2:290908/1‑238 (MQ=255)                                                                                                                                                                                                                              aTGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACACGCGCGCTGGGGATGGCAGAGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGCCACCATTGATTACAGCGTCGGCTTGACTGATATGGCCCGTTTGGGCGACCAGGTAGACGGACAGTGTcctct                  >  2:208679/1‑248 (MQ=255)                                                                                                                                                                                                                                       gCACAAAAAGGCCCAACCGACTTCGTTGAGAACTTCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGGCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCTCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTTGCGGTTAt         <  1:213166/251‑1 (MQ=255)                                                                                                                                                                                                                                              aaGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcgcg  >  1:49485/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCG  >  NC_000913/4618953‑4619440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑