Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,080	G→T	L284L (CTG→CTT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,080	0	G	T	92.9%	107.3 / ‑2.0	42	L284L (CTG→CTT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (17/22);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGG  >  NC_000913/4618836‑4619328                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         tGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTcc                                                                                                                                                                                                                                                    >  1:158906/1‑251 (MQ=255)  gAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCg                                                                                                                                                                                                                                                   >  1:142576/1‑251 (MQ=255)         ggTAGCGGCGCGTTTATGCCGCCCTACGAACTCTCTGAAACCCTTGCCGACGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGAGCACACCCGCGCTGCTCACCGACATGAATCAGGCACTAGCCTCCAATGCTGGTAACGCGGTTGAAGGTCCTGCAGCTGTGCAGTTCCTGACGGGTGAGAATCGTAACCCGCGTCTGATTAAAGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAAcc                                                                                                                                                                                                                                            >  1:62790/1‑250 (MQ=255)         ggTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACt                                                                                                                                                                                                                                            >  1:4183/1‑251 (MQ=255)            tgctgctcgTTGATGCCGACCTACGAACACTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCGGGCGTGCGCGCCCCCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTgg                                                                                                                                                                                                                                          <  2:62722/243‑1 (MQ=255)               ggcgCGTTTATGCCAACCTACGAACTCGCTGAAGCCCTTGCCGAAGCGATGGTTGGCGTGGCTAACGGCGCTGGCGGGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGaa                                                                                                                                                                                                                                      <  2:197078/251‑1 (MQ=255)                        aTGCCGACCTACGAACTCTCTGAAGTCCTGGCCGACGCGATTGTTGGCGTGGCTAACGGCGCTGGCGGGCGCACCACCGCTCTTCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAATGTGGGTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACg                                                                                                                                                                                                                              <  1:152774/250‑1 (MQ=255)                             gACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAg                                                                                                                                                                                                                        <  1:62176/251‑1 (MQ=255)                              aCCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACAGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGATCGTGAAGCGGTACAGTTCCTGACGGGAGAGTAGCGTAACCGGCGTCTGTTTGATGTCACGATGGGGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGCAgc                                                                                                                                                                                                                       >  1:215494/1‑251 (MQ=255)                                     aaCTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGcgcgcgcg                                                                                                                                                                                                                >  1:265970/1‑251 (MQ=255)                                              acagcctggCCGAAGCGATTGTTGGCTTGGCTAACGGCGCGGGCGTGCGCCCCACCGCGCTGCTCAACGACATGAATCAGGTACTGGCCGACAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCaa                                                                                                                                                                                                       <  2:116673/243‑2 (MQ=255)                                                gCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCaagc                                                                                                                                                                                                     >  1:303845/1‑248 (MQ=255)                                                 cccTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCaagc                                                                                                                                                                                                     >  1:209973/1‑247 (MQ=255)                                                  ccTTGCCAAAGAGATTGTTGGAGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGGACGGGCCGCCAGGGCAGGGAACGCGGTTGAAGTTCGTGAAGGGGTGAAGGTCCTGACGGGAGAGTATCGTAACCCGCGTCTGTTTGCTGGCACGATGGCGCTGGGCGTGGAGATGCTTATGTCCGGGAAACTGGCGAAAGATGAAGCCGAAGAGCGTGCGAAATTGCaagg                                                                                                                                                                                                     >  2:382663/1‑246 (MQ=255)                                                        cGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGGTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTAACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAAATGACGCCGAAGCGCGCGCGGAAtttcaagcagagctgg                                                                                                                                                                                             >  2:355008/1‑237 (MQ=255)                                                          aaGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGAc                                                                                                                                                                                           <  1:81720/251‑1 (MQ=255)                                                           aGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCg                                                                                                                                                                                                                                                   <  1:232084/194‑1 (MQ=255)                                                             cGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTTCAAGCAGTGCTGGACAAc                                                                                                                                                                                        <  1:102146/251‑1 (MQ=255)                                                                          ggCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCa                                                                                                                                                                            <  1:225853/250‑1 (MQ=255)                                                                          ggCTAACGGCGCGGTCGTGCGCACCACCGCTCTGCTCACAGACATGAATAAGGTACGGGCCACCAGTGCAGGTAACGCGGTTGAATTTCGTGAAGCGGTGCAGTTCCTGACGGGTTAGTAGCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGATGGACACCGGTAAAGCGGCAg                                                                                                                                                                           <  2:26565/251‑1 (MQ=255)                                                                              aacggggctggctggctccccaccGTGTTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTTCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCTGTAGAAGt                                                                                                                                                                       <  2:265970/232‑1 (MQ=255)                                                                                 ggCGCTGGCGGTCGCACCGCCGCGCTGCTCACCGACAGTAATCAGTGACGGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGTAGCGGGGCAGTTCCTGACGGGTGAGGATCGTAACCCGCGTCTGGTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGGCAACTGTAAAGCGGCTGAAGTCtt                                                                                                                                                                    <  2:131375/251‑1 (MQ=255)                                                                                        gCGTGCGCACCGCCGCGCTGCCTACCGACAGGAATCACGTCGTAGCCCCCAGTGCGGGTAACGCGGTTGAAGTTCTTGAAGCGGTGCATGTCCTGACTGGTTATTTTCGCAACCCGCGTCTGTTTTATGGCACGATGGTGCTTTGCGTGGAGATGGTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAATCGCTCGCGAAATTGCAAGGAGTGCTTGACAACGGTAAAGCGGccgttttccttggtcgt                                                                                                                                                             <  2:195598/251‑17 (MQ=255)                                                                                            gcgcACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATg                                                                                                                                                          <  1:75343/250‑1 (MQ=255)                                                                                            gcgcACCACCGCGCTGCTCACCGAAATTAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATgg                                                                                                                                                         <  1:8588/251‑1 (MQ=255)                                                                                              gcACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGt                                                                                                                                                        >  1:154844/1‑250 (MQ=255)                                                                                                                      agAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGACGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCcaaccgac                                                                                                                               <  1:323068/250‑1 (MQ=255)                                                                                                                                         cAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTAGACGTATGGTAGCGGCACAAAAAGGCCCAACAGACTTCGTTGAGAACTAcgcg                                                                                                             >  1:10913/1‑250 (MQ=255)                                                                                                                                                ggTAACGCGTTTGAAGTTCGTGAATCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCt                                                                                                     <  1:305478/251‑1 (MQ=255)                                                                                                                                                 gTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTg                                                                                                    >  1:244688/1‑251 (MQ=255)                                                                                                                                                                       aGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAg                                                                              <  1:294395/251‑1 (MQ=255)                                                                                                                                                                             gCAGTTCCTGGCGGGTGTGTATCGTAACCCGCGTCTGTTTTATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGCCAACGGTAAAGCGGCAGAAGTCTTTGTTCGTATGGTAGCGTCACAAAAATGCCCAACCGTCTTCCTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCt                                                                        <  1:190678/251‑1 (MQ=255)                                                                                                                                                                                gTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATg                                                                     >  1:227065/1‑251 (MQ=255)                                                                                                                                                                                  tCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCt                                                                   >  1:81265/1‑251 (MQ=255)                                                                                                                                                                                       actggtgagtaccttAACCCGTGTCTTGTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAc                                                              <  2:4183/237‑1 (MQ=255)                                                                                                                                                                                                 atCGGAACCCGCGTCTGTTTGATGTCACGAGGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAAGTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCCCAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGttt                                                     <  2:196819/250‑1 (MQ=255)                                                                                                                                                                                                         ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGa                                            <  1:355008/251‑1 (MQ=255)                                                                                                                                                                                                             gTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGCTGCTGACGAAAGCAGCCTATGCTGATACCGAAGGTTTTGTCAGTGAAATg                                        >  1:373975/1‑251 (MQ=255)                                                                                                                                                                                                                         tCACGATGGCGCTGTGCGTGGAGATTCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGAACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCcgcgcg                            >  1:182770/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTgg            <  1:26669/251‑1 (MQ=255)                                                                                                                                                                                                                                            ggAGATGCTTAGCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTGTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTGTTGTCTGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg         <  2:337035/251‑1 (MQ=255)                                                                                                                                                                                                                                                   cTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGGATGGGAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCCGGGGATGGCCGGGGTTGCAATggg  >  1:254916/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         TGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGG  >  NC_000913/4618836‑4619328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑