Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,717	T→G	A163A (GCT→GCG)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,717	0	T	G	91.7%	42.3 / ‑1.3	24	A163A (GCT→GCG)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (11/11);  total (12/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

TGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCA  >  NC_000913/4618471‑4618959                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     tgttgATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGTTCACTGGCTCCGGCTGAt                                                                                                                                                                                                                                                   <  1:197277/250‑1 (MQ=255)        aaaCATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGtt                                                                                                                                                                                                                                            <  1:384639/250‑1 (MQ=255)           cattCCACTGGGGGCGTCGGCGTTGTGACTCCGCTGATGTTGGGGCCGATGGTCGCTGCCTGCGGCGGCTATATTCCGGTGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTCCGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTa                                                                                                                                                                                                                                        <  2:156017/248‑1 (MQ=255)                        gCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGTGat                                                                                                                                                                                                                          <  1:200833/251‑1 (MQ=255)                          gTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGGTGACAACCGTTTCCGCGGAATTATTAAAAACGTCGGCGTGGCGGTTATC‑GGTTCGACCAGCTCACTGGGTCCGGCGGAGAAAAGTTTTTACCCC‑ACCCGTGgtat                                                                                                                                                                                                                        >  1:129708/1‑251 (MQ=255)                                          cGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGGATCCATCCCTGGCTTCGACATTTTCCCCGATGACAACCGTTTCCGCGAAATTATTTAAAACGGCGGCGTGGCGATTCTCCGGCCA‑AACAACTCCCTTGATCCCGCGGATAAACTTTTCTACGCG‑ACCCCGGATATTAcccacaccctggcc                                                                                                                                                                                                        >  1:305705/1‑239 (MQ=255)                                              gaggTTGGGGCTGATGGTCCCCGCCTGCGGCGGCTATATTCCGTGGAGCTCTGGTCGCGGCCACGGTCTTACTGGCGGTACGCTCGACAAACTGGAAGCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGTTTATC‑GGTCATCCCAGCTCACTGGCTCCGGCGGATAACCGTTTCTACGCG‑ACCCGTGATATTCCCGCAACCGTGGAC‑Tc                                                                                                                                                                                                     <  2:116576/246‑1 (MQ=255)                                                         cGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTAACCTCGACAAACTGGAATACATCCCTGGCTTCGGCATTTTCCCCGATGACAACCGGTTCCGCGAAATTATTAAAAACGTCGGCGTGGCGATTTTC‑GGTCAGACCAGCTCACTGGAGCCGGCGGGTAAAAGCTTTTACGCGCACCC‑TGATATTTCCGCCACCGTgggcttctacccccctgat                                                                                                                                                                                         >  2:148799/1‑234 (MQ=255)                                                                                   tttCCTATGATCTCTGGTGGCGGCCTCGGCCATTGTGGCGGTCCGCCCTCCAAACTGGAATCCACCCCTGGCTTCGACATGTCCCCGAATGACACCCGTTACCGCGAATTTATTAAGGCCGTGGGCGTGGCGTTTATC‑GGTCAGACCAGCTAACTGGCCCCGGCGGATAACCGTTTCTACGCG‑CCCCGTGATATTCCCGCACCCGGGAAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAAc                                                                                                                                                               <  1:275766/249‑1 (MQ=255)                                                                                                ctGGGCGCGGCCTCGGTCATACTGGCGGTACGCTCGAAAAACTGGAATCCACCCCGGGCTTCGACATTTCCCCGGATGACACCCGTTTCCGCGAAATTATTAAAGCCGTCGGCGTGGCGATTATC‑GGTCAGCCCAGCTCACTGGCTCCGGCGGATAAACTTTTCTACGCG‑CCCCGTGATATTCCCGCAACCGTGGAC‑TCCACCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAg                                                                                                                                                      <  1:148799/246‑1 (MQ=255)                                                                                                               gTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGAGATATTAACGCCACCGTGGAC‑TCCATCCCGCTGCTCCCCGCCTCTATTCTGGCTGAGAAACTGGCGGAAGGTCTGGACGCGCTGGGGATg                                                                                                                                  >  1:276909/1‑251 (MQ=255)                                                                                                                        gctgTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGTAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaa                                                                                                                          >  1:197078/4‑250 (MQ=255)                                                                                                                                   aaCAAACTGGAACCCATCCCTTGCTGCTATATTTTCCCGGATCACATCCTTTTCCGCAAAATTATTAAAGGCGTCTGCGTGGCTATTATC‑GTTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACTCG‑CCCCGTGATTTTCCCCCAACCG‑GGACCTCCACCCCGCTGACCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgcgg                                                                                                              <  1:125547/250‑1 (MQ=255)                                                                                                                                          gggAATCCATCCCTGCTTTCAACATTTTCCCGGATGCCAACCGTTCCCGAGAAATTATTAAAGGCGCCGGCGGGGCGTTTATC‑GGTCAGACCAGCTCACTGGCGCCGGCGGATAACCTTTTCTACGCG‑ACCTGTTATATTGCCGCACCCGTGGCC‑GCCTTCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                                                                                                       <  1:48242/250‑1 (MQ=255)                                                                                                                                                ccatcaCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGGGAAAGTGGGTAGCGGCGCGTTTATGCCg                                                                                                 >  1:304051/1‑251 (MQ=255)                                                                                                                                                    cccTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGACATTATTAAAGACCTCGGCCTGGCGATTATC‑GGTCACACCAGCCCCCTGGCTCCGGCGGATAAACGTTTCTCCGCG‑ACCAGAGATATTACCGCAACCGTGGAC‑TCCACCCCGCTGATCACCGCCTCTATTCTGGCGAAGAACCTGGCGGAAGGTCTGGTAGCGCTGGGGATGGACGTGAACGAGGGTAGCGGGGCGTTTATGGCGAccc                                                                                             >  1:165713/1‑250 (MQ=255)                                                                                                                                                    cccTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGATCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATggcggcct                                                                                             >  1:271611/1‑244 (MQ=255)                                                                                                                                                           ttCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACAGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGGAct                                                                                      >  1:149201/1‑251 (MQ=255)                                                                                                                                                                               aacccGTTTCGGCGAAATTATAAAATACGTCGGCGTGGCAATTATC‑GGTAAGACCAGCTCACTGCCTCCGGCGGATAAACTTTTCTACGCG‑CCCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAg                                                                  <  1:249160/248‑1 (MQ=255)                                                                                                                                                                                                          cGTCGGCGTGGCTATTATC‑GTTCAGCCCACCTCACTGCCTCCGGCGGAAAAACGTTTCTACGCG‑CCCCGTGATATCACCGCACCCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTg                                       <  1:286872/251‑1 (MQ=255)                                                                                                                                                                                                                       aTTATC‑GGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGcaccac                          >  1:23199/1‑251 (MQ=255)                                                                                                                                                                                                                                  aGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTc                >  1:116673/1‑251 (MQ=255)                                                                                                                                                                                                                                      cagctCACTGGCTCCGGAGGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTACCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCg            >  1:312900/5‑251 (MQ=255)                                                                                                                                                                                                                                                ccaccGGCGGATAAACGTTTCTACGGG‑ACCCGTGATATTCCCGCACCCGTGGAC‑TCCACCCCGCTTATCACCGCCTCTATTCTGGCGAAGAAACTGGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCa  <  1:88629/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     TGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATC‑GGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTACGCG‑ACCCGTGATATTACCGCAACCGTGGAC‑TCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCA  >  NC_000913/4618471‑4618959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑