Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,489	C→T	T87T (ACC→ACT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,489	0	C	T	85.7%	107.8 / 10.6	50	T87T (ACC→ACT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base T (21/21);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

ACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTA  >  NC_000913/4618240‑4618731                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    acaAGAAATTATTCGTAAAAAACGTGATGGGCATGCGCTGAGCGATGAGGAGATTCGTTTCTTTATCAACGGTATCCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACAATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACAATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAACCCCTGAATCTGAATGGCCCGATTGTTGATAAACACTCCACCg                                                                                                                                                                                                                                                    >  2:152873/1‑251 (MQ=255)          tattCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTc                                                                                                                                                                                                                                            <  1:92958/250‑1 (MQ=255)          tattCGTAAAAAACGTGAGGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAGCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTc                                                                                                                                                                                                                                            <  2:181613/250‑1 (MQ=255)                tAAAAAACGTGATGGTCATGCACTGAGAGATGAAGCAATTCGTTTCTTTATCAAAGGCATTCGCGACAACACTATCTCCAAAGGGCAAATTGACGCCCTCGCGATGACCATTTTCTTCCACGATATGAACATGCCTGAGCGTGTCTCGCTGACCATGACGATGCGAGATTCAGGACCCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGGATGTTGATAAACATTCCACTGGTGTCGTAGGCgctg                                                                                                                                                                                                                                     >  2:384639/1‑248 (MQ=255)                   aaaaCGTGATGGTCTTTCGCTGAGCTATTAAGAAATGCTTTTCTTAATAAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAGTTTCTTCCACGATATGACAATGCCTGAGCGTGCCGCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGa                                                                                                                                                                                                                                  <  2:110091/251‑1 (MQ=255)                   aaaaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAA‑GCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGAc                                                                                                                                                                                                                                 >  1:63836/1‑251 (MQ=255)                    aaaCGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGAc                                                                                                                                                                                                                                 <  1:135826/251‑1 (MQ=255)                    aaaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGc                                                                                                                                                                                                                                               >  2:178028/1‑237 (MQ=255)                    aaaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGc                                                                                                                                                                                                                                               <  1:178028/237‑1 (MQ=255)                      aCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACtt                                                                                                                                                                                                                               >  1:197205/1‑251 (MQ=255)                               tCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGt                                                                                                                                                                                                                      >  1:348669/1‑251 (MQ=255)                                     acTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGc                                                                                                                                                                                                                <  1:269412/250‑1 (MQ=255)                                          gCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATg                                                                                                                                                                                                           >  1:368499/1‑251 (MQ=255)                                                     aTTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTg                                                                                                                                                                                                >  1:381655/1‑251 (MQ=255)                                                                      cggaaTTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGCCAATTTTCTTACACGATATGACAATGCCTGAGCGTGTCTAGCTGACCCTGGCGATGCGAGATTCAGGAACCGATCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGAATGTTGATAAACACTACCCAAGTGACGTCGGCGATGTGACTTAGCTTCTGTTGGGGCCGGTGTTTGCAGCCTACggcggcgatttgccgt                                                                                                                                                                               >  2:29304/5‑241 (MQ=255)                                                                       ggcaTTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGACTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCgat                                                                                                                                                                              <  1:101144/248‑1 (MQ=255)                                                                                        tATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTCTATTCCGATGATCTCTGGTCGCGGcc                                                                                                                                                             >  1:127502/1‑251 (MQ=255)                                                                                           ctcCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCg                                                                                                                                                          >  1:156017/1‑251 (MQ=255)                                                                                           ctcCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCg                                                                                                                                                          >  1:152265/1‑251 (MQ=255)                                                                                               gAAGGGCAGAGTGCCGGCCTCGTGATGACCATTTTTTGCCACTATATGACAATGCCTGAGCGTGGCTCGCTGACCATGGCGATGCGATATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGTTGATCTCTGGTCGCGGCCTCGGTCa                                                                                                                                                      <  2:17340/251‑1 (MQ=255)                                                                                                 aGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCAGGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGAGTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATAGTCCGATTATCTCTGGTCGCGGCCTCGGTCATa                                                                                                                                                    <  2:381655/251‑1 (MQ=255)                                                                                                     cAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTgg                                                                                                                                                >  1:232834/1‑251 (MQ=255)                                                                                                                 cTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCgg                                                                                                                                    >  1:61131/1‑250 (MQ=255)                                                                                                                     cgATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACaa                                                                                                                                 >  1:62722/1‑250 (MQ=255)                                                                                                                      gATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGGCGCGGCCTCGGTCATACTGGCGGGACGCTCGACAAAc                                                                                                                               >  1:26565/1‑251 (MQ=255)                                                                                                                        tGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCgat                                                                                                                                                                              <  1:122738/202‑1 (MQ=255)                                                                                                                                            atGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTc                                                                                                                                                                   <  1:222789/193‑1 (MQ=255)                                                                                                                                            atGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCCCGGCAAACTTGGATCC‑ATCCCTGGCTTCgc                                                                                                        >  1:323714/1‑250 (MQ=255)                                                                                                                                            atGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCAGATGGTCGCAGCCTGCGGCGGCTTTATTCCGGTGATCTCTgggc                                                                                                                                                                   >  2:222789/1‑191 (MQ=255)                                                                                                                                               aCAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGGCGCGGCCTCGGTCATACTGGCGGTACGCCCGACAAACAGGAATGC‑ATCCCCTGCTTTGACAt                                                                                                     >  1:338594/1‑251 (MQ=255)                                                                                                                                                          gCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATg                                                                                          <  1:99295/251‑1 (MQ=255)                                                                                                                                                          gCGTGGCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCGCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATg                                                                                          <  1:269952/251‑1 (MQ=255)                                                                                                                                                           ccgtacccGCTGACCTTGGCGATGCGTGATTCAGGAACCGTTCTCGATTGGAAAATCCTGCATCTGAATGGCCCGATTTTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACGGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGa                                                                                         <  2:378475/244‑1 (MQ=255)                                                                                                                                                               tctcGCTGACCATGGAGATGCGAGATTCAGGCACCGTTCTCGACTGGAAAAGCATGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGCTGTTGGGGCCGATGGTCGCAGCCTGCGGCAGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGTCCACCTGGAATAC‑ATCCCTCGCCTCGACATTTTCACTGATGACAAc                                                                                     >  1:154980/1‑251 (MQ=255)                                                                                                                                                                  cGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCCCGACAAACTGGAATCC‑ATCCCCGGCTTCGACATTTTCCCgggtgtagattgg                                                                                  >  1:378475/1‑240 (MQ=255)                                                                                                                                                                     tGACAATGGCGATGCGAGATTCAGGATCTGTTCTCGACTGGAAAATCCTGCATCTGAATGGCCCGATTGTTGATAATCATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCTATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGTATTC‑ATCCCTAGCTTCGACATTTTCCCGGATGACACCCGttt                                                                                <  1:212344/250‑1 (MQ=255)                                                                                                                                                                                          cAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCTCGACAAACTAGAATCCCACCCCTGG‑TTCGGCCTTTTCCCGGGTGGCCACCGTTTCCGCGGAATTATTTAAGaagac                                                          >  1:347948/1‑247 (MQ=255)                                                                                                                                                                                           aGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCg                                                         <  1:263752/251‑1 (MQ=255)                                                                                                                                                                                             gAACCGTTCTCGACTGGAAAAGCCTGCATCTGAAGGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGc                                                       <  1:142229/251‑1 (MQ=255)                                                                                                                                                                                                  gTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGc                                                  <  1:285950/251‑1 (MQ=255)                                                                                                                                                                                                     ctcGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACAGGCGGTAAGGGCGAGAACATGGAAACC‑ATACCTGGCTTCCGCATTTTCACGGATGACCAACGTTTCCGCGCAAATATTTAAGACGTAGGCggggggg                                                >  1:352388/1‑244 (MQ=255)                                                                                                                                                                                                       cGACTGGAAAAGCCTGCATCGGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGGCAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTa                                             <  1:29304/251‑1 (MQ=255)                                                                                                                                                                                                                     tGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCCTCGACATTTTTCCGGGAGACAACCGGTTCCGCGGAATTATTAAAGACGGCGGGGGGGCGGTTATCGGTCAGACCAgc                               >  1:131375/1‑250 (MQ=255)                                                                                                                                                                                                                            gAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTgg                        <  1:63213/251‑1 (MQ=255)                                                                                                                                                                                                                            gAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCa                            <  1:152873/247‑1 (MQ=255)                                                                                                                                                                                                                                   ccGATTGTTGAGTAACATTCCACTGGGGTCGTCGGTGATTTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTTCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTGTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTTACTGGCTCCGGc                 <  2:152265/251‑1 (MQ=255)                                                                                                                                                                                                                                   cagATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGc                 <  1:174427/249‑1 (MQ=255)                                                                                                                                                                                                                                        tgttgATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGAt             <  1:197277/250‑1 (MQ=255)                                                                                                                                                                                                                                               aaaCATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGtt      <  1:384639/250‑1 (MQ=255)                                                                                                                                                                                                                                                  cattCCACTGGGGGCGTCGGCGTTGTGACTCCGCTGATGTTGGGGCCGATGGTCGCTGCCTGCGGCGGCTATATTCCGGTGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTCCGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTa  <  2:156017/248‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    ACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTA  >  NC_000913/4618240‑4618731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑