Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,420	T→A	A229A (GCT→GCA)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,420	0	T	A	89.7%	106.4 / ‑6.2	39	A229A (GCT→GCA)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (14/21);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGG  >  NC_000913/4175‑4660                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ttACCGAATGTGAAAGTGGTTATCATCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGAGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGGTTAACATCAG‑CCGTTTGCTGGCGCGGATTTGCTACTACTTTGAAGCAGTTgc                                                                                                                                                                                                                                             >  2:278192/1‑251 (MQ=255)  tACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTgc                                                                                                                                                                                                                                             <  1:167430/250‑1 (MQ=255)           ttaaaatGGTTATCCTCTATACACGCGGAAAAATCAGTCCCCTGCAAGACAACCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATACCTTTCAGGCGCTGGTGAAGCAGGCGTTAGCTGCTGAAGAGAC‑GAAAGTGGCGCTGGGGTTCAACTCAACTAACTCGATTAACATCAG‑CCGATTGCTGGCGCCGATTTGCTCCTACTTTCAAGCAGTTTCACAGCtaccc                                                                                                                                                                                                                                   >  2:193592/7‑247 (MQ=255)           tgcaaGTGGTTAACCTCTATCCACGAGGCAAAATCAGTACACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATAGAAACTGTTGCCCTCGACGGCGCTATCGATGCCTGTCAGTAGCTGGTACCGCAGGCGCTTAATGATGAAGA‑TCAGATAGAAGCGCTGGAGTTAAACTCAGCACACTCGCTTAAAAT‑AGTCCGGTTGCTGACGCGGATTAGATACTACTTTGAAGAAGTTGCGCAGCAGCCg                                                                                                                                                                                                                                   >  2:290174/4‑251 (MQ=255)                 tGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGga                                                                                                                                                                                                                              >  1:123750/1‑250 (MQ=255)                          tctAGCCACGAGTCAAAAACAGTCCACTGCAAGAAAAACTGTTCTGTACATTTGGCGGCAATATCGAAGCTTTTGCCTTCGACGGCGAGTTCGATGCCTGTCAGGCGCTGGTGAAGTAGGCGTTTTATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGAGTTGCTACTACGTTGAAGCTTTTGCGCAGCTGCCGCAGGAGACGCGCaa                                                                                                                                                                                                                     <  2:185463/250‑1 (MQ=255)                                    aGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTg                                                                                                                                                                                                          <  1:81442/251‑1 (MQ=255)                                    aGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTg                                                                                                                                                                                                          <  1:305933/251‑1 (MQ=255)                                       tacaaTCAGTACACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGCTGCCTGTCAGGCGCTGGCGAAGCAGACGTTTGATGATGAAGA‑GCTGAAAGAGGCGCTGGGGTAAAACTCAGCTAGCTCGATTAACATCCG‑CCGTTTGCTGGCGCAGTTTTGCTACTACTTTGAAGCAGTTGCGTAGCTGCCTCCGGAAGCGCGCAACCAGCTGGTTGtcc                                                                                                                                                                                                       >  2:46827/4‑250 (MQ=255)                                        aaaaTCAGTCCACTGCAAGAATAACTGTTCTGTACATGGGGCGGCAATATCGAAACTGTTGCCATCGTCGGCGATTTCGATGCCTGTCATGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAATCTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCTGGAAGCGCGCAACCAGCTGGTTGtctc                                                                                                                                                                                                      <  1:297756/251‑1 (MQ=255)                                              aGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGcc                                                                                                                                                                                                <  1:48573/251‑1 (MQ=255)                                                        aaGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAAc                                                                                                                                                                                      >  1:254807/1‑251 (MQ=255)                                                           aaaaaCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCAAAGCGGAAACTTc                                                                                                                                                                                   <  1:250836/251‑1 (MQ=255)                                                            aaaaCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCCG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCg                                                                                                                                                                                  >  2:130479/1‑251 (MQ=255)                                                                  gTTCTGTACATTGGGCGGCAATATCGAAACTGTGGCCAGCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGTCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGAt                                                                                                                                                                             <  1:23410/250‑1 (MQ=255)                                                                    tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCTCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTAGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCAGCAGGAAACGCGCAACCAGCTGGCTGACTCGGGGCCAAACggcacatccggcgattt                                                                                                                                                                           >  2:303599/1‑235 (MQ=255)                                                                              gggCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCggggt                                                                                                                                                                >  1:205800/1‑249 (MQ=255)                                                                               ggcggcAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCTGTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTct                                                                                                                                                               <  2:318297/251‑1 (MQ=255)                                                                                gctgaaagttCGAAACTGTTGCATTGGAAGGCGATTTCGATGCCTGTCAGGCGCTGGTGACGCAGTCGTTGGCGGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAACCTCTGCTAACGCTTTTAACAGCAG‑CCGTTTGCTGTCGCATATTTGCGACTCCTCTGAAGCAGTTGCGCATCTGCTGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCTGAAACTTCGGCGATTTGACGGCGGGTctg                                                                                                                                                              <  2:286160/242‑1 (MQ=255)                                                                                          cGAAACTGTTGCCATCGGCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCTGCGATTTGACGGCGGGTCTGCTGGCGAAGt                                                                                                                                                    <  1:110911/251‑1 (MQ=255)                                                                                          cGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCACCCAGCTGGTTGTCTCGGTGCAAAGCGGAACCTTCGGCGATTTGACGGCgggggaggcgggcgg                                                                                                                                                      >  2:104392/1‑237 (MQ=255)                                                                                               cTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCggggcttcttggggaggcactc                                                                                                                                               >  1:124867/1‑232 (MQ=255)                                                                                                       aTCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCTTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACGCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCTCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGcc                                                                                                                                       <  1:344346/251‑1 (MQ=255)                                                                                                       aTCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGcc                                                                                                                                       <  1:278192/251‑1 (MQ=255)                                                                                                         cGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAGGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCgg                                                                                                                                     <  1:12810/251‑1 (MQ=255)                                                                                                         cGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCg                                                                                                                                      <  1:130479/250‑1 (MQ=255)                                                                                                           aCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGTGAAGTCACTCGGTCTGCCGGTg                                                                                                                                   <  1:287844/251‑1 (MQ=255)                                                                                                           aCGGCAATTTAGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCCGCTAACTCGATTAACATCAG‑CCGTTTTCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTTTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTg                                                                                                                                   <  2:285941/251‑1 (MQ=255)                                                                                                              gCGATTTCGATGCCGAGCAGGCGGTGGGGAAGCAGGCGTTTGAGGAGTAAGA‑ATTTAAAGTGGCTGTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTGGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCTAATTCACTCGGTCTGCCGGTgtaa                                                                                                                                <  2:376435/251‑4 (MQ=255)                                                                                                                                                  cGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTg                                                                                            <  1:115642/251‑1 (MQ=255)                                                                                                                                                                   gcTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                            <  1:272998/250‑1 (MQ=255)                                                                                                                                                                       aaaGTGTCGCTGGGGTTAAACTCATCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATGTGCTACTACTTGGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCCCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCGGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTAtt                                                                                                                       <  2:277142/204‑1 (MQ=255)                                                                                                                                                                       aaaGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCCGGCTGCCGGGGAAACGGTTTAtt                                                                                                                       >  1:277142/1‑204 (MQ=255)                                                                                                                                                                             gcgcTGGGGTAAAACTCAGCTAACGCGTTTAACATCAG‑CGGTTTGCTGGCGCAGTTTTGCTACTACTTTGAAGATGTGGCGGAGGTGCCGCAGGAAGCGCGCAACGAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGTCGATTTGACGGCGGGACTGCTGGCGAAGTCGCTCGGTCTGCCGGTGATACGTTTGATTGCTGCGACCATCGGGAACGATTCCGTGCCACGTGTCCTGCTCGACGGTCAGTg                                                                  <  2:380182/251‑1 (MQ=255)                                                                                                                                                                                             cagCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTCCTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCGCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAATCGTTTTATTGCTGCGACCAACGTGAACGATGCCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                  <  1:167880/249‑1 (MQ=255)                                                                                                                                                                                                       gATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGCAGTAACTCGGGTTGCCGGGGAAAAGTTTTATTGCTGCGACCAACGTGAACGATAACGTGCCACGGTTCCTGCACGACGGTCAGTGGTCACCCCAAGCGAATCaagggccgt                                        >  1:342669/1‑243 (MQ=255)                                                                                                                                                                                                             cATCCG‑CCGTTTGCTGGCGCAGATTTGCTTCTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCa                                  <  1:287415/251‑1 (MQ=255)                                                                                                                                                                                                                         tGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGGTGGCGAAGACACTCCGGCTGCCGGTGGAACGGTTTTTTTCTGCGACCAACGTGAAAGATACCGTGCCACGTTTCCTGCACGAAGGTCAGTGGTCACCCCAAGCCACTCAGGCGACGCTTTCCCACGCGATGGAc                       >  1:285498/1‑251 (MQ=255)                                                                                                                                                                                                                                              actTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCCGGGGGAGGCCGTCGGGCTGCCGGGGAAACGTTTTATTTGTGCGACCAACGTGAACGATACCGTGCCACGTTTTCTGCACGACGGTCAGGGGTCACCCAAAACGACTACGGCGGCGTTATCCAACTCGATGGagggggtggaaccggacaaatgg  >  1:272451/1‑229 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 TTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGG  >  NC_000913/4175‑4660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑