Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	939	G→A	V201V (GTG→GTA)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	939	0	G	A	92.0%	59.0 / ‑2.8	26	V201V (GTG→GTA)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (16/7);  total (19/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGAT  >  NC_000913/710‑1181                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   gTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGac                                                                                                                                                                                                                               >  1:77444/1‑251 (MQ=255)     cGAGAAAATGACGATCGCCATTATGGCAGGCGTATTAGAAGCGCGCGGTAACAACGTTACCGTTATCGATACGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGCTATTGCTGCGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCAAATGGTGCTGATGGCAGGTTTACCCTCCGGTAATGAAAAAGGCACACTGGTGGTGATTGGGCGGAAAGGTTCCGactacc                                                                                                                                                                                                                           >  2:44618/1‑250 (MQ=255)            aTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgcggc                                                                                                                                                                                                                    >  1:96321/1‑245 (MQ=255)                cGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACACCGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGAAATGAAAAAGGCGAACTGGTGGTACTTGGGCGCAACGGTTCCGACTCCTCCGCGGCGGTg                                                                                                                                                                                                                >  2:150776/1‑251 (MQ=255)                 gATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTACTGAAAAAGGCGAACTGGGGGAACTTGGACGAAACGGGTCCGaatactacgcggcggggg                                                                                                                                                                                                               >  2:208118/1‑239 (MQ=255)                     gCCATTATGGCCGGCGTATTAGAAGCGCGCGGGCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTACGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTCCGCGGCGGAgctggg                                                                                                                                                                                                           >  1:318659/1‑250 (MQ=255)                      ccATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAACAACTGCTGGCAGAGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGATGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTgctggct                                                                                                                                                                                                          >  1:44685/1‑251 (MQ=255)                              gCCGGCGTATTAGAAGAGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTggtt                                                                                                                                                                                                  >  1:329575/1‑248 (MQ=255)                                      aTTAGAAGCGAGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAACATGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGTGTCCACCCGCCGTATTGAGGAAAGTCGCATTCCGGCTGATCCCATGGTGCTGATGGCAGGTTTAACCGCCGGTAATGAAAAAGGCGTACTGGTGGTCCTGGGACGCAACGGTTCAGACTACGCCGCGGCGGTGAGGGCTACCTGTTTACGCGCCg                                                                                                                                                                                          >  2:226888/1‑251 (MQ=255)                                                           cGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCAGACTACTCCGCGGCGGTGCTGGCTGCCTGATTGAGCGGCGATTTTTGCGAGATTTggacgg                                                                                                                                                                     >  2:272852/1‑251 (MQ=255)                                                                                           tgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGATTCCACCAGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGATTCACCGCCGGTAATGAAACAGGCGAACTGGTGGTACTTGGAAGAAACGGTTCCGACTAATCCGCGGCGGTGCTCGCTGGCTGGTTACTCGACGATTGTTACGGGATGTGGACGTACGTTGAGGGGGGCTTAACCTGCGAccccgtg                                                                                                                                     >  2:90780/1‑247 (MQ=255)                                                                                                       aTTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGAt                                                                                                                         >  1:105616/1‑251 (MQ=255)                                                                                                                   cTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTTTTGCGGGATTTGGGCGGACGTTGACGGGGTCTATACCTGCGACCCACGGCCGGTGCCCGATGCGAGGttgttg                                                                                                             >  1:338988/1‑251 (MQ=255)                                                                                                                           gATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCCCCGATTGTTGCGCGATTTTGCCGCACGGTGCCGGGGTCTATACATGAGCCCCCCGTCAGGGGCCCGATTCGAAGTTGATGGAGTCggt                                                                                                     >  1:247810/1‑249 (MQ=255)                                                                                                                                                   aTTGCGGCAAGTCGCATCCCGGCTGATCACAGGGTGCTGATGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGc                                                                              <  1:93164/250‑1 (MQ=255)                                                                                                                                                         gCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTACTGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGCTGGAGCTTTCCt                                                                        >  1:129529/1‑250 (MQ=255)                                                                                                                                                             gtcGCATTCCGGTTGATCACATGGTGCGGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTc                                                                   <  1:208118/249‑1 (MQ=255)                                                                                                                                                                          tGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACCCGCCGATTGTTGCGAGGTTTGGAGGGGCGTTGGCGGGGTCTATACCTTCGACCCGCGGCAGGGGCCCGATGCGGGGGTTTTGAAGCCGATTCCCTACCAGGAAGGGATGGAGCTTTCCCATTTCAGCGTTAAAGttt                                                      >  1:178431/1‑250 (MQ=255)                                                                                                                                                                              cacaTGGTGCTGAGGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCa                                                  <  1:54952/251‑1 (MQ=255)                                                                                                                                                                                        tGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGGTTGTTGCGGGATTTGGACGGACGTTGACGGGGGCTTTTCCTGCGACCCGCGTCAGGTGCCCGATGCggggg                                                                                                                  >  1:197309/1‑173 (MQ=255)                                                                                                                                                                                              cAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTaac                                  >  1:10682/1‑249 (MQ=255)                                                                                                                                                                                                    tCACCGCCGGTAATGAAAAAGGCGAACTGGTGTTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCAt                             <  1:204621/250‑1 (MQ=255)                                                                                                                                                                                                       ccgccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCTCGcc                         >  1:373718/1‑251 (MQ=255)                                                                                                                                                                                                        cgcaggTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTTCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGccc                        <  2:96321/247‑1 (MQ=255)                                                                                                                                                                                                         gccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCa                       <  1:166970/251‑1 (MQ=255)                                                                                                                                                                                                                                ggtggtACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGAt  <  1:44618/249‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   GTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGAT  >  NC_000913/710‑1181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑