Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,618,055 C→T L245L (CTG→TTG)  deoC → deoxyribose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,618,0550CT97.9% 136.7 / ‑5.3 48L245L (CTG→TTG) deoCdeoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (27/20);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCG  >  NC_000913/4617831‑4618296
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
ccGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGt                                                                                                                                                                                                                         >  1:108886/1‑251 (MQ=255)
ccGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGt                                                                                                                                                                                                                         >  1:177552/1‑251 (MQ=255)
      aaGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGAGACGGTAAGAgc                                                                                                                                                                                                                   >  1:214402/1‑251 (MQ=255)
         tGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTAAAAACAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCCCTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGcc                                                                                                                                                                                                                >  1:236040/1‑251 (MQ=255)
          ggCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGATCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTGCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCca                                                                                                                                                                                                               <  2:58718/251‑1 (MQ=255)
                    gcgACGCCGGAAAGCGCGCGCATCATGATGGCAGTGATCCGTGATATGAGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGCCGGTAAGAGCGCCAGCAGctact                                                                                                                                                                                                      >  2:278166/1‑250 (MQ=255)
                      gaccgcGGAAAGCGCGTGCATCATGAGGGAAGTGATGCGTGATATGTGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGTGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGATCGTTTCGTTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACtaag                                                                                                                                                                                                   <  2:271164/246‑1 (MQ=255)
                      gACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCCGcaacta                                                                                                                                                                                                     >  2:374673/1‑249 (MQ=255)
                            ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCTCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGAt                                                                                                                                                                                             <  1:267889/251‑1 (MQ=255)
                            ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAAACGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGGTGCGCCGAAATATCTCGCCATTGCCGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCAATACCGCTTTGGCGCTTCCAGCCTGCTGGGAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTaagaaaga                                                                                                                                                                                              >  2:135826/1‑250 (MQ=255)
                                aGCGCGCGTATCATTATGGAAGTGATCCGTGAGAGGGGCGTAGAAAAATACTTTGGTTTCAAACCAGAGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCTTCGCAGATGAACGGGTCGGTGCTGACTGGGCAGAGGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCtt                                                                                                                                                                                         <  2:354218/251‑1 (MQ=255)
                                   gcgcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGAc                                                                                                                                                                                                                            <  1:292608/213‑1 (MQ=255)
                                   gcgcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGAGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTAAAAGCGCAgggccaaggtgca                                                                                                                                                                                                                            >  2:292608/1‑203 (MQ=255)
                                         atcatGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTg                                                                                                                                                                                 >  1:161852/1‑250 (MQ=255)
                                            ttgatgGAAGTGATCCGTGATATGGGCGAAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGAGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGAATGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGGGACGGTAAGAGCGCCAGAAGCTACTAAGAAAGATGCTTTACGCCTGAtgc                                                                                                                                                                             >  2:267468/2‑251 (MQ=255)
                                                    aGTGATCCGTGAGAGGGGAGTAGAACAAACCGTTGGTTGCAAACCAGCGGGCGGCGTGCGTACTGCGGATGATGCTCAGAAATATCTCGCCCTCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCATCAGCTACTAAGTAAGATGCTTTAGGCCTGATgcgctgcgct                                                                                                                                                                     <  1:310169/251‑1 (MQ=255)
                                                       gATCCGGGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTAt                                                                                                                                                                  <  1:291850/251‑1 (MQ=255)
                                                          ccGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATc                                                                                                                                                                 >  1:190955/1‑249 (MQ=255)
                                                           cgttatatGGGCGTAGAAAAAACCGTTGGTTTCAAGCCAGCGGGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAgg                                                                                                                                                              <  1:20723/247‑1 (MQ=255)
                                                             ggatctGGGCGTAGAAAAAACCGTTTGTTTCACACCAGCGGGCTGCGTTCGTACTGCTGAAGATTCGCCGAAATATCTCGCCATCGCAGATGAACTGTTCTGTGCTGCCTGTGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCATCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTCTACGCCTGATGCGCTGCTCTTATCAGGcc                                                                                                                                                            <  2:72879/246‑1 (MQ=255)
                                                               atatGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCt                                                                                                                                                           >  1:190641/1‑250 (MQ=255)
                                                                tatGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTAc                                                                                                                                                         >  1:4671/1‑251 (MQ=255)
                                                                    ggCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGCCGGAAAGAGCGCCAGCAGCTACTAGGTAAGATGCTTTACGCCTGGTGCGCTGCGCTTCTCAGGCCTACgagc                                                                                                                                                     >  2:181006/1‑250 (MQ=255)
                                                                    ggCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACg                                                                                                                                                        >  1:247431/1‑248 (MQ=255)
                                                                         aGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTa                                                                                                                                                 <  1:270027/250‑1 (MQ=255)
                                                                                 ccGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGt                                                                                                                                        >  2:269412/1‑251 (MQ=255)
                                                                                     tGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATAGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGAGCTGCGCTTATCAGACCTACGAGACGTATCTACTCGTAGGc                                                                                                                                    >  1:236849/1‑251 (MQ=255)
                                                                                                 agcgggcggCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTAACTACCGCTTTGGTGCTTCCAGACTGCTGGCAAGCCTGTTGAAAACGCTGGGCCAAGGTGACGGTAAGAGCGCCAGCAGCTACTAAGAAATATGCTTTACGCATGATGCGCTGCACTTATCAGGCCAACGAGACGTATCTACCCGTAGGCCGGATAAGCCGt                                                                                                                        >  2:282715/2‑251 (MQ=255)
                                                                                                           gtgcgtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATcc                                                                                                              <  1:181006/251‑1 (MQ=255)
                                                                                                                                      tatCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTcc                                                                                   >  1:231664/1‑251 (MQ=255)
                                                                                                                                         ctcGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCg                                                                                >  1:134946/1‑251 (MQ=255)
                                                                                                                                          ccGCCATCGCAGATGAACTGTGCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCgg                                                                               <  1:313857/250‑1 (MQ=255)
                                                                                                                                              catcgCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCCGGCCTACGAGACGTATCTACCAGTCGGCCGGATAAGGCGTAGACGCATCCGGCCAAAGCCGCCTCATACTCTTTTCCTCGGGAgg                                                                           >  2:92958/5‑251 (MQ=255)
                                                                                                                                                 cgCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGGGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCAAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGCCGCACCCGGCAAACGCCGCCTAATACTCATTTCCTCGGGAGGtt                                                                         >  2:78225/2‑250 (MQ=255)
                                                                                                                                                                    gCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGcaca                                                        >  1:132770/1‑248 (MQ=255)
                                                                                                                                                                            ggCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCCGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTc                                             >  1:107378/1‑251 (MQ=255)
                                                                                                                                                                             gCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCg                                            <  1:278166/251‑1 (MQ=255)
                                                                                                                                                                               agaggcgtgTCACTACCTCTTTGGTGCTTCCAGCTAGCTGTCAATCCTGTTGAAAGCGCTGGGCCTCGGTGACTGTAAGAGCGCCAGCAGTTTCTAAGTAATATGCTTTACGCCTGAGGCGCTGTGCTTATCAGGCCTACGAGACGTATCTACCCTTTGGCCGGATAATGCGTAGACGCATCCGGCAAAAGCAGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCTCACAAGAAATTTTTCGTa                                          <  2:346731/243‑1 (MQ=255)
                                                                                                                                                                                   gcgcGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTaaaa                                       >  1:127793/1‑250 (MQ=255)
                                                                                                                                                                                             ccctgtttggttcttcaggCCTGCTGGTACGCCTGTTGAAAGCGCTGGGCCACGGGGACGGTAAGAGCACAAGCATCGACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTCCCCCTAGGCCGTATAAGGCGTAGACGCATCCGGCAAAAGCTGACTCATACTCTTGTCCTCGGGAGGTTATCTGGTTTCTCGCACAAGAAATTATTCGTAGAAAACGTGATGGt                            <  2:77214/233‑1 (MQ=255)
                                                                                                                                                                                             acctctttgggtcTTCCAGCCAGCTGGCAAGCCTGTTTAAAGCGCTGGGCCCCGGTGACGTTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATgg                             <  2:4671/238‑1 (MQ=255)
                                                                                                                                                                                                           tCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCg               <  1:267468/250‑1 (MQ=255)
                                                                                                                                                                                                            ccAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGATGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCAGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGAt             >  1:113592/1‑251 (MQ=255)
                                                                                                                                                                                                             gagattgctgGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCAAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTATGCCGGATAAGGCGTAGACGCCTCCGGCACAAGCCGCATCATACTCTTTTCATCGGGATGTTAACTTGATTCTCGCACAAGAAATTATTCGTAAAAAACATGGTGGTCaagaactgatagatg            >  2:285950/6‑237 (MQ=255)
                                                                                                                                                                                                               gCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATga           >  1:110091/1‑250 (MQ=255)
                                                                                                                                                                                                                   gctgGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATgaagaa       <  1:263516/250‑1 (MQ=255)
                                                                                                                                                                                                                     gggctaGCCTGTTGAAAGCTCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAAtt    <  1:207655/246‑1 (MQ=255)
                                                                                                                                                                                                                       gCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCg  <  1:225642/251‑1 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
CCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCG  >  NC_000913/4617831‑4618296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑