Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,976	T→C	I218I (ATT→ATC)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,976	0	T	C	97.4%	108.6 / ‑4.5	40	I218I (ATT→ATC)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (21/17);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

AGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGG  >  NC_000913/4617733‑4618219                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aGTGATCAGCGACACGGGCGAAATGAAAGACGACGCTGTGCTCAGTAAAGCGGATGACATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGATAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGAGCCGTGATAGGGGCGTAGAAAAAACCGATGGTTACAAACCAGCGGGCGGCGAGCGTACGGCAGAAGATGCGCAGAAATATCTCGCCATCGCAGATg                                                                                                                                                                                                                                              <  2:106400/251‑1 (MQ=255)           gAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTCCGGACCTCATAAAAACCTCTACCGGAAAAGCGGCTGTGAACGCGACGCCGGAAAGCGCGCACATCAAGATGGAACTGATCCGTGGTATGGGCGGAGAAAAAACCGTTGCTTTCAAACCCGCGGGCGGCGTGCGGACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGGTGaaatggtctg                                                                                                                                                                                                                                    >  1:44664/1‑243 (MQ=255)                  gCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCACAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGAc                                                                                                                                                                                                                             >  1:346539/1‑251 (MQ=255)                                         aTCCGTAAAGCGGCTGAAAGAGCCAGCAAAGCCGGTGCGGACGTCATCAAAACCACATCCGGTTAAGGGGCTGTGAACTCGACGCTGGACAGCGCGCGCATCATGATGGAAGTGATCCGTGATAAGGGCGTAGAAAAAACCGTTGGTTTCCAACCAGCGGGCGGCGTGCGTACTGCGGAAGAGGCGCAGAAATATTTCGCCTTCTCAGATGAACTGTTCGGTGCGGACTTGGCATATGCGCGTGACTAcct                                                                                                                                                                                                      <  2:326711/251‑2 (MQ=255)                                           ccGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCt                                                                                                                                                                                                    <  1:74447/251‑1 (MQ=255)                                                                       gcggtgtcGGACTTAATCAAAACCTCTACCGGTAAAGTGGCTGTGGACGCGACGGCGGAACTCGCGCGCACCATGATGGAAGTGAGCCGTGATATGGGCTTAGAAAAAACCTGTGGTTGTAACCCAGCCGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAAGATCTCGCCATCGCAGATGAACTGTTCTGTGCTGACTGGGCAGATGCGCGTCACTACCGTTTTGGTTCTGCCAGCCGGCTGGCAAGCct                                                                                                                                                                        <  2:358988/244‑1 (MQ=255)                                                                         gggTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCctgt                                                                                                                                                                      >  1:58718/1‑250 (MQ=255)                                                                          ggTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCctgtt                                                                                                                                                                     <  1:276612/251‑3 (MQ=255)                                                                                                   ccGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGt                                                                                                                                            >  1:108886/1‑251 (MQ=255)                                                                                                   ccGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGt                                                                                                                                            >  1:177552/1‑251 (MQ=255)                                                                                                         aaGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGAGACGGTAAGAgc                                                                                                                                      >  1:214402/1‑251 (MQ=255)                                                                                                            tGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTAAAAACAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCCCTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGcc                                                                                                                                   >  1:236040/1‑251 (MQ=255)                                                                                                             ggCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGATCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTGCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCca                                                                                                                                  <  2:58718/251‑1 (MQ=255)                                                                                                                       gcgACGCCGGAAAGCGCGCGCATCATGATGGCAGTGATCCGTGATATGAGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGCCGGTAAGAGCGCCAGCAGctact                                                                                                                         >  2:278166/1‑250 (MQ=255)                                                                                                                         gaccgcGGAAAGCGCGTGCATCATGAGGGAAGTGATGCGTGATATGTGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGTGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGATCGTTTCGTTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACtaag                                                                                                                      <  2:271164/246‑1 (MQ=255)                                                                                                                         gACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCCGcaacta                                                                                                                        >  2:374673/1‑249 (MQ=255)                                                                                                                               ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCTCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGAt                                                                                                                <  1:267889/251‑1 (MQ=255)                                                                                                                               ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAAACGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGGTGCGCCGAAATATCTCGCCATTGCCGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCAATACCGCTTTGGCGCTTCCAGCCTGCTGGGAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTaagaaaga                                                                                                                 >  2:135826/1‑250 (MQ=255)                                                                                                                                   aGCGCGCGTATCATTATGGAAGTGATCCGTGAGAGGGGCGTAGAAAAATACTTTGGTTTCAAACCAGAGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCTTCGCAGATGAACGGGTCGGTGCTGACTGGGCAGAGGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCtt                                                                                                            <  2:354218/251‑1 (MQ=255)                                                                                                                                      gcgcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGAc                                                                                                                                               <  1:292608/213‑1 (MQ=255)                                                                                                                                      gcgcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGAGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTAAAAGCGCAgggccaaggtgca                                                                                                                                               >  2:292608/1‑203 (MQ=255)                                                                                                                                            atcatGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTg                                                                                                    >  1:161852/1‑250 (MQ=255)                                                                                                                                               ttgatgGAAGTGATCCGTGATATGGGCGAAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGAGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGAATGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGGGACGGTAAGAGCGCCAGAAGCTACTAAGAAAGATGCTTTACGCCTGAtgc                                                                                                >  2:267468/2‑251 (MQ=255)                                                                                                                                                       aGTGATCCGTGAGAGGGGAGTAGAACAAACCGTTGGTTGCAAACCAGCGGGCGGCGTGCGTACTGCGGATGATGCTCAGAAATATCTCGCCCTCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCATCAGCTACTAAGTAAGATGCTTTAGGCCTGATgcgctgcgct                                                                                        <  1:310169/251‑1 (MQ=255)                                                                                                                                                          gATCCGGGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTAt                                                                                     <  1:291850/251‑1 (MQ=255)                                                                                                                                                             ccGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATc                                                                                    >  1:190955/1‑249 (MQ=255)                                                                                                                                                              cgttatatGGGCGTAGAAAAAACCGTTGGTTTCAAGCCAGCGGGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAgg                                                                                 <  1:20723/247‑1 (MQ=255)                                                                                                                                                                ggatctGGGCGTAGAAAAAACCGTTTGTTTCACACCAGCGGGCTGCGTTCGTACTGCTGAAGATTCGCCGAAATATCTCGCCATCGCAGATGAACTGTTCTGTGCTGCCTGTGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCATCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTCTACGCCTGATGCGCTGCTCTTATCAGGcc                                                                               <  2:72879/246‑1 (MQ=255)                                                                                                                                                                  atatGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCt                                                                              >  1:190641/1‑250 (MQ=255)                                                                                                                                                                   tatGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTAc                                                                            >  1:4671/1‑251 (MQ=255)                                                                                                                                                                       ggCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGCCGGAAAGAGCGCCAGCAGCTACTAGGTAAGATGCTTTACGCCTGGTGCGCTGCGCTTCTCAGGCCTACgagc                                                                        >  2:181006/1‑250 (MQ=255)                                                                                                                                                                       ggCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACg                                                                           >  1:247431/1‑248 (MQ=255)                                                                                                                                                                            aGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTa                                                                    <  1:270027/250‑1 (MQ=255)                                                                                                                                                                                    ccGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGt                                                           >  2:269412/1‑251 (MQ=255)                                                                                                                                                                                        tGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATAGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGAGCTGCGCTTATCAGACCTACGAGACGTATCTACTCGTAGGc                                                       >  1:236849/1‑251 (MQ=255)                                                                                                                                                                                                    agcgggcggCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTAACTACCGCTTTGGTGCTTCCAGACTGCTGGCAAGCCTGTTGAAAACGCTGGGCCAAGGTGACGGTAAGAGCGCCAGCAGCTACTAAGAAATATGCTTTACGCATGATGCGCTGCACTTATCAGGCCAACGAGACGTATCTACCCGTAGGCCGGATAAGCCGt                                           >  2:282715/2‑251 (MQ=255)                                                                                                                                                                                                              gtgcgtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATcc                                 <  1:181006/251‑1 (MQ=255)                                                                                                                                                                                                                                         tatCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTcc      >  1:231664/1‑251 (MQ=255)                                                                                                                                                                                                                                            ctcGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCg   >  1:134946/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccGCCATCGCAGATGAACTGTGCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCgg  <  1:313857/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    AGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGG  >  NC_000913/4617733‑4618219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑