Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,928	G→A	P202P (CCG→CCA)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,928	0	G	A	97.4%	109.2 / ‑4.2	40	P202P (CCG→CCA)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (21/17);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGC  >  NC_000913/4617688‑4618166                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               gAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGgcggcg                                                                                                                                                                                                                                      <  1:64970/251‑1 (MQ=255)                     gcgcGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATgcg                                                                                                                                                                                                                  >  1:273226/1‑251 (MQ=255)                     gcgcGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATgc                                                                                                                                                                                                                   <  1:383780/250‑1 (MQ=255)                                 aTGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGACGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAAAAtctc                                                                                                                                                                                                      >  1:273739/1‑251 (MQ=255)                                    tACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGCAGCGCTGATCCGAAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATAATGATGGAAGTGATCCGAGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGCTGCGCAGCAATATCTCGcc                                                                                                                                                                                                   >  1:161722/1‑251 (MQ=255)                                              aGTGATCAGCGACACGGGCGAAATGAAAGACGACGCTGTGCTCAGTAAAGCGGATGACATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGATAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGAGCCGTGATAGGGGCGTAGAAAAAACCGATGGTTACAAACCAGCGGGCGGCGAGCGTACGGCAGAAGATGCGCAGAAATATCTCGCCATCGCAGATg                                                                                                                                                                                         <  2:106400/251‑1 (MQ=255)                                                        gAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTCCGGACCTCATAAAAACCTCTACCGGAAAAGCGGCTGTGAACGCGACGCCGGAAAGCGCGCACATCAAGATGGAACTGATCCGTGGTATGGGCGGAGAAAAAACCGTTGCTTTCAAACCCGCGGGCGGCGTGCGGACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGGTGaaatggtctg                                                                                                                                                                               >  1:44664/1‑243 (MQ=255)                                                               gCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCACAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGAc                                                                                                                                                                        >  1:346539/1‑251 (MQ=255)                                                                                      aTCCGTAAAGCGGCTGAAAGAGCCAGCAAAGCCGGTGCGGACGTCATCAAAACCACATCCGGTTAAGGGGCTGTGAACTCGACGCTGGACAGCGCGCGCATCATGATGGAAGTGATCCGTGATAAGGGCGTAGAAAAAACCGTTGGTTTCCAACCAGCGGGCGGCGTGCGTACTGCGGAAGAGGCGCAGAAATATTTCGCCTTCTCAGATGAACTGTTCGGTGCGGACTTGGCATATGCGCGTGACTAcct                                                                                                                                                 <  2:326711/251‑2 (MQ=255)                                                                                        ccGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCt                                                                                                                                               <  1:74447/251‑1 (MQ=255)                                                                                                                    gcggtgtcGGACTTAATCAAAACCTCTACCGGTAAAGTGGCTGTGGACGCGACGGCGGAACTCGCGCGCACCATGATGGAAGTGAGCCGTGATATGGGCTTAGAAAAAACCTGTGGTTGTAACCCAGCCGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAAGATCTCGCCATCGCAGATGAACTGTTCTGTGCTGACTGGGCAGATGCGCGTCACTACCGTTTTGGTTCTGCCAGCCGGCTGGCAAGCct                                                                                                                   <  2:358988/244‑1 (MQ=255)                                                                                                                      gggTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCctgt                                                                                                                 >  1:58718/1‑250 (MQ=255)                                                                                                                       ggTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCctgtt                                                                                                                <  1:276612/251‑3 (MQ=255)                                                                                                                                                ccGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGt                                                                                       >  1:108886/1‑251 (MQ=255)                                                                                                                                                ccGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGt                                                                                       >  1:177552/1‑251 (MQ=255)                                                                                                                                                      aaGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGAGACGGTAAGAgc                                                                                 >  1:214402/1‑251 (MQ=255)                                                                                                                                                         tGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTAAAAACAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCCCTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGcc                                                                              >  1:236040/1‑251 (MQ=255)                                                                                                                                                          ggCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGATCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTGCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCca                                                                             <  2:58718/251‑1 (MQ=255)                                                                                                                                                                    gcgACGCCGGAAAGCGCGCGCATCATGATGGCAGTGATCCGTGATATGAGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGCCGGTAAGAGCGCCAGCAGctact                                                                    >  2:278166/1‑250 (MQ=255)                                                                                                                                                                      gaccgcGGAAAGCGCGTGCATCATGAGGGAAGTGATGCGTGATATGTGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGTGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGATCGTTTCGTTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACtaag                                                                 <  2:271164/246‑1 (MQ=255)                                                                                                                                                                      gACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCCGcaacta                                                                   >  2:374673/1‑249 (MQ=255)                                                                                                                                                                            ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCTCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGAt                                                           <  1:267889/251‑1 (MQ=255)                                                                                                                                                                            ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAAACGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGGTGCGCCGAAATATCTCGCCATTGCCGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCAATACCGCTTTGGCGCTTCCAGCCTGCTGGGAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTaagaaaga                                                            >  2:135826/1‑250 (MQ=255)                                                                                                                                                                                aGCGCGCGTATCATTATGGAAGTGATCCGTGAGAGGGGCGTAGAAAAATACTTTGGTTTCAAACCAGAGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCTTCGCAGATGAACGGGTCGGTGCTGACTGGGCAGAGGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCtt                                                       <  2:354218/251‑1 (MQ=255)                                                                                                                                                                                   gcgcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGAc                                                                                          <  1:292608/213‑1 (MQ=255)                                                                                                                                                                                   gcgcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGAGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTAAAAGCGCAgggccaaggtgca                                                                                          >  2:292608/1‑203 (MQ=255)                                                                                                                                                                                         atcatGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTg                                               >  1:161852/1‑250 (MQ=255)                                                                                                                                                                                            ttgatgGAAGTGATCCGTGATATGGGCGAAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGAGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGAATGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGGGACGGTAAGAGCGCCAGAAGCTACTAAGAAAGATGCTTTACGCCTGAtgc                                           >  2:267468/2‑251 (MQ=255)                                                                                                                                                                                                    aGTGATCCGTGAGAGGGGAGTAGAACAAACCGTTGGTTGCAAACCAGCGGGCGGCGTGCGTACTGCGGATGATGCTCAGAAATATCTCGCCCTCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCATCAGCTACTAAGTAAGATGCTTTAGGCCTGATgcgctgcgct                                   <  1:310169/251‑1 (MQ=255)                                                                                                                                                                                                       gATCCGGGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTAt                                <  1:291850/251‑1 (MQ=255)                                                                                                                                                                                                          ccGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATc                               >  1:190955/1‑249 (MQ=255)                                                                                                                                                                                                           cgttatatGGGCGTAGAAAAAACCGTTGGTTTCAAGCCAGCGGGCGGCGGGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAgg                            <  1:20723/247‑1 (MQ=255)                                                                                                                                                                                                             ggatctGGGCGTAGAAAAAACCGTTTGTTTCACACCAGCGGGCTGCGTTCGTACTGCTGAAGATTCGCCGAAATATCTCGCCATCGCAGATGAACTGTTCTGTGCTGCCTGTGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCATCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTCTACGCCTGATGCGCTGCTCTTATCAGGcc                          <  2:72879/246‑1 (MQ=255)                                                                                                                                                                                                               atatGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCt                         >  1:190641/1‑250 (MQ=255)                                                                                                                                                                                                                tatGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTAc                       >  1:4671/1‑251 (MQ=255)                                                                                                                                                                                                                    ggCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGCCGGAAAGAGCGCCAGCAGCTACTAGGTAAGATGCTTTACGCCTGGTGCGCTGCGCTTCTCAGGCCTACgagc                   >  2:181006/1‑250 (MQ=255)                                                                                                                                                                                                                    ggCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACg                      >  1:247431/1‑248 (MQ=255)                                                                                                                                                                                                                         aGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTa               <  1:270027/250‑1 (MQ=255)                                                                                                                                                                                                                                 ccGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGt      >  2:269412/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATAGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGAGCTGCGCTTATCAGACCTACGAGACGTATCTACTCGTAGGc  >  1:236849/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGC  >  NC_000913/4617688‑4618166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑