Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,532	C→T	I70I (ATC→ATT)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,532	0	C	T	100.0%	76.2 / NA	25	I70I (ATC→ATT)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

TTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAT  >  NC_000913/4617294‑4617774                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ttttATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAAc                                                                                                                                                                                                                                          >  1:65004/1‑250 (MQ=255)       gggcaaCAAGCCAGGAAAATGAAATGACTGATCTGAAAGAAAGCAGCCTGCGTGCACTAAAATTGATGGACCTGCCCACCCGG‑AATGACGACGACACCGACGAAAAAGTAATTGCTCTGTGTCATCAGGCAAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTc                                                                                                                                                                                                                                   <  1:278295/246‑1 (MQ=255)             aaaGCAAGGAGAATGAATTATCTGATCTAAAAGCAAGAAGCTTGCGGGCACTGAAATTGATGGACCTGACC‑CCCTGTAATGACGACGACCCCGACGAGAAAGTAATTGCTCGGTGTCACCAGCCCAAACCCCCGG‑GCGACAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCacac                                                                                                                                                                                                                             <  1:272010/250‑1 (MQ=255)                      agaATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAacgat                                                                                                                                                                                                                    >  1:120141/1‑250 (MQ=255)                          tgaaatgaCTGATCTGAAAGCAAGCAGACTGCGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTGCCCACACGGTAacgatgcc                                                                                                                                                                                                                 >  1:82832/1‑246 (MQ=255)                                       cTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCgcgc                                                                                                                                                                                                    >  1:366178/1‑250 (MQ=255)                                                         cGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGc                                                                                                                                                                                 >  2:112977/1‑251 (MQ=255)                                                             cACTGAAATTGAGGGCCCTGCCCCCCCTG‑AAGGACGACGACACCGACGAGAATGTAATTGCTTTGTTTCTCCGGGCCAAAACCCCGG‑TCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATc                                                                                                                                                                             <  1:221741/251‑1 (MQ=255)                                                                            ccatgcccccccTG‑AATGACGACGACCCCGACGAAAAAGTAATTGCTCTGTGTCATCGGGCCAAACCCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgat                                                                                                                                                              <  1:112977/242‑1 (MQ=255)                                                                                                       cccGACGATAAAGTAATTGCGCTGTGACATCAGCCCAAACCCCCGG‑TCGCAAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACGTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCTTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACcg                                                                                                                                    <  1:152271/250‑1 (MQ=255)                                                                                                         cgacgaGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACcgcg                                                                                                                                  >  1:271164/1‑251 (MQ=255)                                                                                                            cgaGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCgcgcgc                                                                                                                               >  1:344025/1‑251 (MQ=255)                                                                                                                                   caccagtcAAAACCCCGGGTCGGCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAggt                                                                                                         <  1:262823/244‑1 (MQ=255)                                                                                                                                                        ggCAATACCGCCGCTATCTGTATCTATCCTAGATTTCTCCCGTTTGCTCGCAAAACACTGCACGAGCAGGCCCCCCAGGAAATCCGTATTCCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGAGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGCCGTTGACGTGGTGTTCCCGTACCGCGCGCTGCTGGCGGTTTACGCGCAggttcgcttttacctctgcgactc                                                                                    >  2:182439/1‑231 (MQ=255)                                                                                                                                                                       atcggTATCTAACCTCGCTTTATCCCGCTTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAAACCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAAGCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGAGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTg                                                                    <  2:262054/247‑1 (MQ=255)                                                                                                                                                                             atctatcCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGATGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTGAAGCGCAGGTTGGGTTT‑ACACTGGTGAAAGCCttgtaagaggcgaggcgagg                                                              >  2:64970/1‑232 (MQ=255)                                                                                                                                                                                      cGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCggaagt                                                     >  1:382374/1‑246 (MQ=255)                                                                                                                                                                                          ttATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAATGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTAct                                                  <  1:338816/250‑1 (MQ=255)                                                                                                                                                                                                  aatgctcgataaccactGAAAGAGCAGGGCACCCCAGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGGAACGATGACATCGAAAGCGCGCTGGCAGAAACCCGTGCGGCAAGCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCATCGAATGTACTGCTGAAAga                                         <  2:120141/236‑2 (MQ=255)                                                                                                                                                                                                     gCTCGCAAAACACTGAAAGAGCCGGGCACCCCGGAAATCCGTATTGCGACGGTAACCAACTGCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGa                                       <  2:245686/250‑1 (MQ=255)                                                                                                                                                                                                            aaaCACTGAAAGAGCAGGGCACCCCGGAAATCCGTAGTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGaa                               <  1:182439/251‑1 (MQ=255)                                                                                                                                                                                                             aacactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGaaa                              <  1:231837/247‑1 (MQ=255)                                                                                                                                                                                                                                    cGGAAATCCGTTTTTCTACGGTAACCAACCTCCCACACGGTTACGAGGACCTCGAAATCGCTCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAgcg       <  1:263416/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAAAGAGCAGGCTGGTTTTGAC‑CTGGGGAAAGCCTGTAAAGAGGCTTGCGCGGCCGCGCATGTACTGATGGAAGGGAACATCGAAACCGGCGCACTggaacacgcacggctg    >  1:367953/1‑235 (MQ=255)                                                                                                                                                                                                                                         aTCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAt  <  1:211004/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    TTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGG‑TCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGAC‑CTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGAT  >  NC_000913/4617294‑4617774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑