Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351	A→G	L206L (CTA→CTG)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351	0	A	G	87.2%	93.6 / 3.0	47	L206L (CTA→CTG)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base G (17/24);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

GATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTG  >  NC_000913/4103‑4596                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACTGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAgg                                                                                                                                                                                                                                                      >  1:376435/1‑251 (MQ=255)               cATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGG‑GCTGAAAGTGGCGCTGGGGTTAAACTcagat                                                                                                                                                                                                                                         >  1:364223/1‑246 (MQ=255)                     gacctcgcccTCCGGTGATACCGGATCGGCAGTGGCTGAGTCTTTCTACGTTTTACCTAATGTGAAAGTGGTTATCCTCTATCCTCGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTGCTGTACAGTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATACCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGTGGTTAAACTCAGCTAACt                                                                                                                                                                                                                                     <  2:147381/240‑1 (MQ=255)                     gACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACt                                                                                                                                                                                                                                     <  1:6515/248‑1 (MQ=255)                       ccGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGAtt                                                                                                                                                                                                                                >  1:318297/1‑251 (MQ=255)                             ccTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGTTGGGGTTAAACTCAGCTAACTCGATTAACATc                                                                                                                                                                                                                          <  1:337411/251‑1 (MQ=255)                              cTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATTGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATc                                                                                                                                                                                                                          >  1:152098/1‑250 (MQ=255)                                 aggTGATACCGGAGCGGCAGTAGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTATGTACATTGGGCGGCAATATCGAAAATGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGGGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGGGGCGCTGGGGTTAAACTCAACTAACTCGATTAAAATCAG‑cc                                                                                                                                                                                                                     >  2:48573/2‑251 (MQ=255)                                        aCCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTACACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCt                                                                                                                                                                                                              >  2:115642/1‑251 (MQ=255)                                        aCCGGAGCGGCAGTGGCTCAAGCTTTCTTAGGGTTACCGAATGTGAAAGTGGCTATCCTCTATCCACGAGGCAAAATCAATCCACTGCAAGAAAAACTGTTATTTACATTGCGAGGCAATATCGAAACTGTTGCCATCGACGGCGCTTTCGATGACTGTCAGGCGCTGGTTAAACAGGCGTTTGAGGTGGCAAA‑GCTGACAGGGGCGCTGGGGTTAAACTAGGCTCACTCGATTAACATCAgtcagttgc                                                                                                                                                                                                                >  2:216401/1‑242 (MQ=255)                                         ccGGAGCGTCAGTGGCTCATGCTTGCTGCGGTTTACCGAATGTGAAAGTGGTTATCCTCTATACATGAGGCAAAATCAGTCCACTGCAAGACAAACTGTTCTGTCCAGTGGGCGGCAATATCGAAACTGTTGTCAACGACGGCGATTTCTATGCCTGTCAGGCGCTGGTGAAGTAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTGACATCaacggttttctg                                                                                                                                                                                                              <  2:206117/251‑12 (MQ=255)                                            taGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAGGTGGCGCTGGGGTTAAACACAGCTAACTCGATTAAAAGCAG‑CCGTTTgtgggcg                                                                                                                                                                                                          >  2:18911/2‑245 (MQ=255)                                               cGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAg                                                                                                                                                                                                       <  1:157115/251‑1 (MQ=255)                                                                         ttACCGAATGTGAAAGTGGTTATCATCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGAGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGGTTAACATCAG‑CCGTTTGCTGGCGCGGATTTGCTACTACTTTGAAGCAGTTgc                                                                                                                                                                             >  2:278192/1‑251 (MQ=255)                                                                          tACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTgc                                                                                                                                                                             <  1:167430/250‑1 (MQ=255)                                                                                   ttaaaatGGTTATCCTCTATACACGCGGAAAAATCAGTCCCCTGCAAGACAACCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATACCTTTCAGGCGCTGGTGAAGCAGGCGTTAGCTGCTGAAGAGAC‑GAAAGTGGCGCTGGGGTTCAACTCAACTAACTCGATTAACATCAG‑CCGATTGCTGGCGCCGATTTGCTCCTACTTTCAAGCAGTTTCACAGCtaccc                                                                                                                                                                   >  2:193592/7‑247 (MQ=255)                                                                                   tgcaaGTGGTTAACCTCTATCCACGAGGCAAAATCAGTACACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATAGAAACTGTTGCCCTCGACGGCGCTATCGATGCCTGTCAGTAGCTGGTACCGCAGGCGCTTAATGATGAAGA‑TCAGATAGAAGCGCTGGAGTTAAACTCAGCACACTCGCTTAAAAT‑AGTCCGGTTGCTGACGCGGATTAGATACTACTTTGAAGAAGTTGCGCAGCAGCCg                                                                                                                                                                   >  2:290174/4‑251 (MQ=255)                                                                                         tGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGga                                                                                                                                                              >  1:123750/1‑250 (MQ=255)                                                                                                  tctAGCCACGAGTCAAAAACAGTCCACTGCAAGAAAAACTGTTCTGTACATTTGGCGGCAATATCGAAGCTTTTGCCTTCGACGGCGAGTTCGATGCCTGTCAGGCGCTGGTGAAGTAGGCGTTTTATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGAGTTGCTACTACGTTGAAGCTTTTGCGCAGCTGCCGCAGGAGACGCGCaa                                                                                                                                                     <  2:185463/250‑1 (MQ=255)                                                                                                            aGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTg                                                                                                                                          <  1:305933/251‑1 (MQ=255)                                                                                                            aGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTg                                                                                                                                          <  1:81442/251‑1 (MQ=255)                                                                                                               tacaaTCAGTACACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGCTGCCTGTCAGGCGCTGGCGAAGCAGACGTTTGATGATGAAGA‑GCTGAAAGAGGCGCTGGGGTAAAACTCAGCTAGCTCGATTAACATCCG‑CCGTTTGCTGGCGCAGTTTTGCTACTACTTTGAAGCAGTTGCGTAGCTGCCTCCGGAAGCGCGCAACCAGCTGGTTGtcc                                                                                                                                       >  2:46827/4‑250 (MQ=255)                                                                                                                aaaaTCAGTCCACTGCAAGAATAACTGTTCTGTACATGGGGCGGCAATATCGAAACTGTTGCCATCGTCGGCGATTTCGATGCCTGTCATGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAATCTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCTGGAAGCGCGCAACCAGCTGGTTGtctc                                                                                                                                      <  1:297756/251‑1 (MQ=255)                                                                                                                      aGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGcc                                                                                                                                <  1:48573/251‑1 (MQ=255)                                                                                                                                aaGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAAc                                                                                                                      >  1:254807/1‑251 (MQ=255)                                                                                                                                   aaaaaCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCAAAGCGGAAACTTc                                                                                                                   <  1:250836/251‑1 (MQ=255)                                                                                                                                    aaaaCTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCCG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCg                                                                                                                  >  2:130479/1‑251 (MQ=255)                                                                                                                                          gTTCTGTACATTGGGCGGCAATATCGAAACTGTGGCCAGCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGTCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGAt                                                                                                             <  1:23410/250‑1 (MQ=255)                                                                                                                                            tCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCTCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTAGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCAGCAGGAAACGCGCAACCAGCTGGCTGACTCGGGGCCAAACggcacatccggcgattt                                                                                                           >  2:303599/1‑235 (MQ=255)                                                                                                                                                      gggCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCggggt                                                                                                >  1:205800/1‑249 (MQ=255)                                                                                                                                                       ggcggcAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCTGTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTct                                                                                               <  2:318297/251‑1 (MQ=255)                                                                                                                                                        gctgaaagttCGAAACTGTTGCATTGGAAGGCGATTTCGATGCCTGTCAGGCGCTGGTGACGCAGTCGTTGGCGGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAACCTCTGCTAACGCTTTTAACAGCAG‑CCGTTTGCTGTCGCATATTTGCGACTCCTCTGAAGCAGTTGCGCATCTGCTGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCTGAAACTTCGGCGATTTGACGGCGGGTctg                                                                                              <  2:286160/242‑1 (MQ=255)                                                                                                                                                                  cGAAACTGTTGCCATCGGCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCTGCGATTTGACGGCGGGTCTGCTGGCGAAGt                                                                                    <  1:110911/251‑1 (MQ=255)                                                                                                                                                                  cGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCACCCAGCTGGTTGTCTCGGTGCAAAGCGGAACCTTCGGCGATTTGACGGCgggggaggcgggcgg                                                                                      >  2:104392/1‑237 (MQ=255)                                                                                                                                                                       cTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCggggcttcttggggaggcactc                                                                               >  1:124867/1‑232 (MQ=255)                                                                                                                                                                               aTCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCTTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACGCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCTCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGcc                                                                       <  1:344346/251‑1 (MQ=255)                                                                                                                                                                               aTCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGcc                                                                       <  1:278192/251‑1 (MQ=255)                                                                                                                                                                                 cGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAGGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCgg                                                                     <  1:12810/251‑1 (MQ=255)                                                                                                                                                                                 cGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCg                                                                      <  1:130479/250‑1 (MQ=255)                                                                                                                                                                                   aCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGTGAAGTCACTCGGTCTGCCGGTg                                                                   <  1:287844/251‑1 (MQ=255)                                                                                                                                                                                   aCGGCAATTTAGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCCGCTAACTCGATTAACATCAG‑CCGTTTTCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTTTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTg                                                                   <  2:285941/251‑1 (MQ=255)                                                                                                                                                                                      gCGATTTCGATGCCGAGCAGGCGGTGGGGAAGCAGGCGTTTGAGGAGTAAGA‑ATTTAAAGTGGCTGTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTGGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCTAATTCACTCGGTCTGCCGGTgtaa                                                                <  2:376435/251‑4 (MQ=255)                                                                                                                                                                                                                          cGTTTGATGATGAAGA‑GCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTg                            <  1:115642/251‑1 (MQ=255)                                                                                                                                                                                                                                           gcTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg            <  1:272998/250‑1 (MQ=255)                                                                                                                                                                                                                                               aaaGTGTCGCTGGGGTTAAACTCATCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATGTGCTACTACTTGGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCCCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCGGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTAtt                                                       <  2:277142/204‑1 (MQ=255)                                                                                                                                                                                                                                               aaaGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCCGGCTGCCGGGGAAACGGTTTAtt                                                       >  1:277142/1‑204 (MQ=255)                                                                                                                                                                                                                                                     gcgcTGGGGTAAAACTCAGCTAACGCGTTTAACATCAG‑CGGTTTGCTGGCGCAGTTTTGCTACTACTTTGAAGATGTGGCGGAGGTGCCGCAGGAAGCGCGCAACGAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGTCGATTTGACGGCGGGACTGCTGGCGAAGTCGCTCGGTCTGCCGGTGATACGTTTGATTGCTGCGACCATCGGGAACGATTCCGTGCCACGTGTCCTGCTCGACGGTCAGTg  <  2:380182/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGA‑ACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAG‑CCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTG  >  NC_000913/4103‑4596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑