Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,616,682	C→T	P128P (CCG→CCA)	yjjI ←	DUF3029 domain‑containing protein YjjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,616,682	0	C	T	85.3%	66.8 / 6.6	34	P128P (CCG→CCA)	yjjI	DUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (15/14);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01

CTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCG  >  NC_000913/4616448‑4616916                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           cttcGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCAt                                                                                                                                                                                                                            >  1:78043/1‑251 (MQ=255)                    agGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTGCGGGGAAACCTGCTTCAACTCTGCGCCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc                                                                                                                                                                                                         <  1:187516/251‑1 (MQ=255)                            aGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGCCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGGAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGGGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                                                                                                                                                                                  >  2:10765/1‑250 (MQ=255)                                 tGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTACGCTCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGGTTTATTCGAATATCGCGCTCGTCTTTTGTTAGCATTCTAACCTATGGTTGCCACACTGCATCCAATTGCCCCAGGTAGACCGGCATcatattg                                                                                                                                                                                            >  2:371512/1‑245 (MQ=255)                                                       ggTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGAACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaa                                                                                                                                                                       >  1:310693/1‑250 (MQ=255)                                                                          tGCTTCAAATCTGCGTATGCACGTACGATAGCGCGGGGAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCCTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGACTATCGATCTTGTCTTGTGTTAGTATTCTAACATATGGGTGCAACAACGCTTCCAGTTGCCCCAGGTCGTCCGGCAACCATGTGAACTACGGTACGTCGTGGTAGAGAATGATcagactaggga                                                                                                                                                    >  2:61167/1‑242 (MQ=255)                                                                                 aCTCTGCGTCGGCACGTAAGATCGCGCGGGTAA‑TGGGCGAAGCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGtca                                                                                                                                            <  1:9165/251‑1 (MQ=255)                                                                                       cgtctgcaCGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTCTCGCCAGAGACGTTTTATTCGAATATCGGTCTCGTCTTGTGTTAGAATTCTAGAATATGGTTGCAACAACGCATCCACATGCAACAGGGAGACCACCCTAGATGTGACCGACGGTACGTGGTAGTAACGATGGGTCAGCAGGGAGGGTTCGtcatcaga                                                                                                                                       >  2:296364/3‑248 (MQ=255)                                                                                            gcccGTAAGGTCGCGGGGGTAAGTGGG‑GAATTAGACGGGCCGATATTGGCGTGCATAAAGGCTGCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATAGCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGTTCttt                                                                                                                                 <  2:203576/248‑1 (MQ=255)                                                                                            gcaCGTAAGATCGCACGGGTAA‑GGGGCGAAACAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGGCGAGGTAACGCAAGAAACGGTTTATTCGAACATCGATCTCGTCTTGTGGTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCAACATCAAGATCtgt                                                                                                                                 <  2:78043/251‑3 (MQ=255)                                                                                                gTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGACTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGTTCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCACGTTGCCCCAGGCAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGGGTGCGTCATAAAGATCTTTcgcg                                                                                                                             >  1:264274/1‑251 (MQ=255)                                                                                                  taGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTCCATAAAGGAGTCTGGCAGGGATCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACAGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGAGTCATCAAGATCTTTCGCGcc                                                                                                                           >  2:89850/2‑251 (MQ=255)                                                                                                   aGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGCAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGCTGTGACCGACGGTACGTGGGGGTAAAGAATGGTCAGCAGAGAGAGTGcagatcggaagcgcgtcgggg                                                                                                                            >  2:8049/1‑229 (MQ=255)                                                                                                   aGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGc                                                                                                                                                <  1:8049/229‑1 (MQ=255)                                                                                                   aGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCt                                                                                                                          <  1:60778/251‑1 (MQ=255)                                                                                                                       cGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCgg                                                                                                       <  1:10765/251‑1 (MQ=255)                                                                                                                       cGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCgg                                                                                                       <  1:296364/251‑1 (MQ=255)                                                                                                                         aaTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCAGCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGaa                                                                                                     <  1:302218/251‑1 (MQ=255)                                                                                                                                         atTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc                                                                                     <  1:13346/251‑1 (MQ=255)                                                                                                                                                   aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATc                                                                           <  1:368032/251‑1 (MQ=255)                                                                                                                                                        ggCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTa                                                                      <  1:94008/251‑1 (MQ=255)                                                                                                                                                               ggCAGGGTTATGTCGAAGTAACGCCAGAAACGTTTTATTCGAATATCGATATCGTCTTGTGTTAGCATTCTAACATATGGTTGCAACAACGCATCCCGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGAGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATATTTCGCGACTTCCAGCTCCAGACATTCGGAACCGTTCGACAGGAAACGGCCGTAATCGGGTAAGACAt                                                                >  2:345831/1‑250 (MQ=255)                                                                                                                                                                gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTATTGTGTTAGAATTCTAACATATGGTTGCCACAACGAATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGCCGGTACGTGGCGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCACGCTCCAGCCATTCGGAACCGTTCGCCAGCAAACGGGCGTAATCGGGTAAGACAt                                                                >  1:159571/1‑249 (MQ=255)                                                                                                                                                                            cGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTAc                                                  >  1:281178/1‑251 (MQ=255)                                                                                                                                                                                                   aTTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTAACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATc                           <  1:89850/251‑1 (MQ=255)                                                                                                                                                                                                     tcgaatataaaTCTCGTCTTATGTTAGAATTCTAACATATGGTTACAACAACGCATCCCGTTGCCCCAGGTAGACCGGCATCGATGTGACCGCCGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGCTCTTTCGCGCCTTCAAGCTACAGACATCCGGAACCGTTCGCCAGAAAACGGGCGTAATAGGGTAAGACATAGCGAGGATTGTACGGAGAATGACCTTCCAAcctaaggc                         >  2:367198/11‑244 (MQ=255)                                                                                                                                                                                                                   cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTc            >  1:180130/1‑250 (MQ=255)                                                                                                                                                                                                                   cGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCTTCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACAcc               <  1:189014/247‑1 (MQ=255)                                                                                                                                                                                                                    gTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCAt          >  1:362570/1‑251 (MQ=255)                                                                                                                                                                                                                     tCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGCTGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTCATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATc         >  1:5601/1‑251 (MQ=255)                                                                                                                                                                                                                      cTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACata                             >  1:347277/1‑230 (MQ=255)                                                                                                                                                                                                                      cTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGATAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAACGGGCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACata                             <  2:347277/230‑1 (MQ=255)                                                                                                                                                                                                                        tgtgTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATcc        <  1:99732/249‑1 (MQ=255)                                                                                                                                                                                                                            ttAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGACGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTCAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgcgcg  >  1:157990/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           CTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCG  >  NC_000913/4616448‑4616916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑