Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,616,648 C→T V140I (GTT→ATT)  yjjI ← DUF3029 domain‑containing protein YjjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,616,6480CT87.1% 66.6 / 3.6 31V140I (GTT→ATT) yjjIDUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (14/13);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

CACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATC  >  NC_000913/4616403‑4616891
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
cACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCg                                                                                                                                                                                                                                                 >  1:116320/1‑250 (MQ=255)
    tgACCGGTGGCGATGTGCGGGTTGCTACATTCACAGAGGGTCTTCGCCACTTCCAGCAGCAGTTCATCAGGTGTGATTTCAGGTTCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATTAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGAt                                                                                                                                                                                                                                               <  2:71815/247‑1 (MQ=255)
    ggACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGAt                                                                                                                                                                                                                                               >  1:71815/1‑248 (MQ=255)
        cGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCt                                                                                                                                                                                                                                        <  1:123357/251‑1 (MQ=255)
                aTGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGGAACGCCAGACACGTTTTATTAGAATATCGATCTCGTCTTGGGtt                                                                                                                                                                                                                                  >  2:105517/1‑249 (MQ=255)
                               cATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATatgg                                                                                                                                                                                                                 >  1:203576/1‑248 (MQ=255)
                                             cttcGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCAt                                                                                                                                                                                                   >  1:78043/1‑251 (MQ=255)
                                                                agGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTGCGGGGAAACCTGCTTCAACTCTGCGCCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc                                                                                                                                                                                <  1:187516/251‑1 (MQ=255)
                                                                        aGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGCCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGGAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGGGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                                                                                                                                                         >  2:10765/1‑250 (MQ=255)
                                                                             tGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTACGCTCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGGTTTATTCGAATATCGCGCTCGTCTTTTGTTAGCATTCTAACCTATGGTTGCCACACTGCATCCAATTGCCCCAGGTAGACCGGCATcatattg                                                                                                                                                                   >  2:371512/1‑245 (MQ=255)
                                                                                                   ggTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGAACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaa                                                                                                                                              >  1:310693/1‑250 (MQ=255)
                                                                                                                      tGCTTCAAATCTGCGTATGCACGTACGATAGCGCGGGGAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCCTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGACTATCGATCTTGTCTTGTGTTAGTATTCTAACATATGGGTGCAACAACGCTTCCAGTTGCCCCAGGTCGTCCGGCAACCATGTGAACTACGGTACGTCGTGGTAGAGAATGATcagactaggga                                                                                                                           >  2:61167/1‑242 (MQ=255)
                                                                                                                             aCTCTGCGTCGGCACGTAAGATCGCGCGGGTAA‑TGGGCGAAGCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGtca                                                                                                                   <  1:9165/251‑1 (MQ=255)
                                                                                                                                   cgtctgcaCGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTCTCGCCAGAGACGTTTTATTCGAATATCGGTCTCGTCTTGTGTTAGAATTCTAGAATATGGTTGCAACAACGCATCCACATGCAACAGGGAGACCACCCTAGATGTGACCGACGGTACGTGGTAGTAACGATGGGTCAGCAGGGAGGGTTCGtcatcaga                                                                                                              >  2:296364/3‑248 (MQ=255)
                                                                                                                                        gcccGTAAGGTCGCGGGGGTAAGTGGG‑GAATTAGACGGGCCGATATTGGCGTGCATAAAGGCTGCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATAGCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGTTCttt                                                                                                        <  2:203576/248‑1 (MQ=255)
                                                                                                                                        gcaCGTAAGATCGCACGGGTAA‑GGGGCGAAACAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGGCGAGGTAACGCAAGAAACGGTTTATTCGAACATCGATCTCGTCTTGTGGTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCAACATCAAGATCtgt                                                                                                        <  2:78043/251‑3 (MQ=255)
                                                                                                                                            gTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGACTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGTTCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCACGTTGCCCCAGGCAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGGGTGCGTCATAAAGATCTTTcgcg                                                                                                    >  1:264274/1‑251 (MQ=255)
                                                                                                                                              taGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTCCATAAAGGAGTCTGGCAGGGATCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACAGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGAGTCATCAAGATCTTTCGCGcc                                                                                                  >  2:89850/2‑251 (MQ=255)
                                                                                                                                               aGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGCAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGCTGTGACCGACGGTACGTGGGGGTAAAGAATGGTCAGCAGAGAGAGTGcagatcggaagcgcgtcgggg                                                                                                   >  2:8049/1‑229 (MQ=255)
                                                                                                                                               aGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGc                                                                                                                       <  1:8049/229‑1 (MQ=255)
                                                                                                                                               aGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCt                                                                                                 <  1:60778/251‑1 (MQ=255)
                                                                                                                                                                   cGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCgg                                                                              <  1:10765/251‑1 (MQ=255)
                                                                                                                                                                   cGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCgg                                                                              <  1:296364/251‑1 (MQ=255)
                                                                                                                                                                     aaTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCAGCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGaa                                                                            <  1:302218/251‑1 (MQ=255)
                                                                                                                                                                                     atTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc                                                            <  1:13346/251‑1 (MQ=255)
                                                                                                                                                                                               aTAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATc                                                  <  1:368032/251‑1 (MQ=255)
                                                                                                                                                                                                    ggCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTa                                             <  1:94008/251‑1 (MQ=255)
                                                                                                                                                                                                           ggCAGGGTTATGTCGAAGTAACGCCAGAAACGTTTTATTCGAATATCGATATCGTCTTGTGTTAGCATTCTAACATATGGTTGCAACAACGCATCCCGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGAGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATATTTCGCGACTTCCAGCTCCAGACATTCGGAACCGTTCGACAGGAAACGGCCGTAATCGGGTAAGACAt                                       >  2:345831/1‑250 (MQ=255)
                                                                                                                                                                                                            gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTATTGTGTTAGAATTCTAACATATGGTTGCCACAACGAATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGCCGGTACGTGGCGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCACGCTCCAGCCATTCGGAACCGTTCGCCAGCAAACGGGCGTAATCGGGTAAGACAt                                       >  1:159571/1‑249 (MQ=255)
                                                                                                                                                                                                                        cGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTAc                         >  1:281178/1‑251 (MQ=255)
                                                                                                                                                                                                                                               aTTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTAACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATc  <  1:89850/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
CACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAA‑TGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATC  >  NC_000913/4616403‑4616891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑