| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,616,535 | A→G | D177D (GAT→GAC) | yjjI ← | DUF3029 domain‑containing protein YjjI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,616,535 | 0 | A | G | 96.9% | 92.1 / ‑4.6 | 33 | D177D (GAT→GAC) | yjjI | DUF3029 domain‑containing protein YjjI |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/16); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01 | |||||||||||
CGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCA > NC_000913/4616291‑4616778 | gggCAATTGCTTTCAGGGTAAGGTGTACCAGCTCTCGGTCACTCCCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTAAATTCACAGATGTTCTTCGCCACGTCCAGCAGCAGGGCATCAGGGGTGATTTCAGGATCGGAGATAAAGGTCAGGTCAGGGGAAACCTGCTTCAACtctgcgtctgca < 2:294445/250‑1 (MQ=255) gTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCggg < 1:162259/251‑1 (MQ=255) gTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTAAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCggg > 1:274068/1‑251 (MQ=255) tAAGGCGTACCAGCGTGCTGCCCCCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGt < 1:92630/250‑1 (MQ=255) gTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTCCATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGAGTCTGCACGTAAGATCGAGCGGGTAATggcc > 2:121966/1‑249 (MQ=255) aCCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCg > 1:330235/1‑250 (MQ=255) aCCAGCGTGCTGCCACAACCCGCCAGCGGCAGAGAATTGTAACAGCTCACAATCCCGAAGCCCCCTTTTGTGAAAAATTTATCACGCCCC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACCGATGTTCTTCGCCCCTTCCAGCAGCAGGTCATCAGTGGTGATTTCAGGATCGTAGATAACTGTCAGGTTCGGGGAATCCTGCTTCTACTCGGCGTCTGCACGTCAGATCGCGAGGGTACTGGGca > 2:189014/1‑249 (MQ=255) gctggtgtcccccccccGCCAGCGGGAGTGAGTTGTCATTGTTCACACTCCCGTATGCTCTTTTTTTGAAAATATTTTCAGGCTCC‑TGAACGTTGGCGATGTGGGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCCTCAGGGGTGATTTCAGGATCGTAGATAAAGTTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGTGCGAATCa < 2:324739/237‑1 (MQ=255) gctgcCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACg < 1:59906/251‑1 (MQ=255) ccccaccCGCCAGCGGCAGTGAGTTGTAACATCTCACAATCCCGTAGCCCCCTTGTGTGAAAAGTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTGTGCTACATTCACAGATGTTCGTCGCCACGTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCGGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCg < 2:32587/249‑1 (MQ=255) ggCAGTGAGTTGTAACAGCTCACAATCCCTTAGCCCCCTTTTGTGAAAATTTTATCATTCACC‑GGTCCGTTGGCGATGTGCGGTTTTCTACATTCACAGATTTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTGCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCAt < 1:16418/251‑1 (MQ=255) cAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATaa < 1:363307/251‑1 (MQ=255) gtaGTTGTAACATCCCACAATCCCGTAGCCCCCTTTGTTGAAAATTTTATCAT‑CACCCGGGCCGGTGGCTATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAgg < 2:3238/249‑1 (MQ=255) gAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAgg < 1:121966/250‑1 (MQ=255) cgTTGTCACAGCTCTCAATCTCGCAGCCCCCGTGTGAGAAAATTTTATCTTGCACC‑GGACCGTGGGCGATGTGATGTTTTGTACATGCACAGAGGTTCTTCGCCACTTCCAGCAGCAGTTCATCAGGGGTTATTTCAGGATCGTTTATAAAGGTCAGGTTCGGGGAAACCTGCTTCGTCTCTGCGTCGGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGTCGATATTGGCTTGCATAAAGGc < 2:336462/249‑1 (MQ=255) ccgCTCACAATCCCGTAGCCCCCATATGTGAAAATTTTATCATGAACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCAACTTCCAGCAGCAGGTCAGCAGGGGTGATTTCAGGATCGTAGATAAAGGTAAGGATCGGGGCAACATGCTTCATCTCTGCGTCTGCACGCAAGATAGCGCGAGGAATGGGCGAATAAGACGGTCCGATATGGGCCAGCATAAAGGCGTcaggagg > 2:138180/3‑245 (MQ=255) cccGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGACTGGCAGGGTTCTGTCgg > 2:368032/1‑250 (MQ=255) gTAGCCCCTTTGTGTGAAAATTTTATTATGCCCT‑GGACCTTTGGCGATGTGCGGGTTGCTACTGTCACATATGGTTGTCGCCCCTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGACAAAGGTCAGGTTCGGGGAAACCTGCTGCAACTCTGCTTCTGCACTTAAGACTGCGCGGGTAATGGGCGAATCAGAGGGGCCGATATTGGCGTGCATAAAGGCGTCTTGCAGGGTTCTGTCGAGGt < 2:330235/251‑1 (MQ=255) gCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGCCCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCGTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAAc < 1:159633/251‑1 (MQ=255) aaTTTTATCATGCTCC‑GGACCGTTGGCTATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCCCTTCCAGCAGCAGGTCATCATGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGGCTGCACGTAATATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAATCGTTTTa < 1:156905/251‑1 (MQ=255) ttATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGGCGAGGTAACGCCAGAAACGTTTTATTCg > 1:8730/1‑251 (MQ=255) cACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCg > 1:116320/1‑250 (MQ=255) tgACCGGTGGCGATGTGCGGGTTGCTACATTCACAGAGGGTCTTCGCCACTTCCAGCAGCAGTTCATCAGGTGTGATTTCAGGTTCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATTAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGAt < 2:71815/247‑1 (MQ=255) ggACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGAt > 1:71815/1‑248 (MQ=255) cGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCt < 1:123357/251‑1 (MQ=255) aTGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGGAACGCCAGACACGTTTTATTAGAATATCGATCTCGTCTTGGGtt > 2:105517/1‑249 (MQ=255) cATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATatgg > 1:203576/1‑248 (MQ=255) cttcGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCAt > 1:78043/1‑251 (MQ=255) agGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTGCGGGGAAACCTGCTTCAACTCTGCGCCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc < 1:187516/251‑1 (MQ=255) aGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGCCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGGAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGGGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc > 2:10765/1‑250 (MQ=255) tGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGCACGTACGCTCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGGTTTATTCGAATATCGCGCTCGTCTTTTGTTAGCATTCTAACCTATGGTTGCCACACTGCATCCAATTGCCCCAGGTAGACCGGCATcatattg > 2:371512/1‑245 (MQ=255) ggTCAGGTTCGGGGAAACCTGCTTCAACTCTGCGTCTGAACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTaa > 1:310693/1‑250 (MQ=255) tGCTTCAAATCTGCGTATGCACGTACGATAGCGCGGGGAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCCTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGACTATCGATCTTGTCTTGTGTTAGTATTCTAACATATGGGTGCAACAACGCTTCCAGTTGCCCCAGGTCGTCCGGCAACCATGTGAACTACGGTACGTCGTGGTAGAGAATGATcagactaggga > 2:61167/1‑242 (MQ=255) aCTCTGCGTCGGCACGTAAGATCGCGCGGGTAATGGGCGAAGCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGtca < 1:9165/251‑1 (MQ=255) | CGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACC‑GGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTGCGTCA > NC_000913/4616291‑4616778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |