Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,602,089	C→T	V3V (GTG→GTA)  *257* (TGA→TAA)	yjjB ← yjjP ←	putative succinate exporter YjjB putative succinate exporter YjjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,602,089	0	C	T	88.6%	75.8 / 2.3	35	V3V (GTG→GTA)  *257* (TGA→TAA)	yjjB yjjP	putative succinate exporter YjjB putative succinate exporter YjjP
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (19/12);  total (20/15)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

GATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCG  >  NC_000913/4601845‑4602334                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gagtcgacgactacccgctcgACCATTAAATACCATTGGTCCCGACCTGCATAGAAGCCATAAAGGTGTACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGGAACGCCCGCACGGGAACGTTGAACTCCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAAGTCGATTACAccc                                                                                                                                                                                                                                                 <  2:314162/232‑1 (MQ=255)                           tGAATACCAATGGTACCGACCAGCATAGAAGCCATCCAGGTTGAACACTCAATATTCAACACGCTGATCAACAAGATCATTCGGGACCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTAAACACCATCGCAAAGCCGAACGCAGGACGGGCGGCGAGGATCAACACCTGCGCCAAACCGACCAGAGAGTCGATTACACACATCTGCGTAACCGCCAAATCGTCAGt                                                                                                                                                                                                                       >  2:35490/1‑251 (MQ=255)                                   aaTGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGGTTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCAc                                                                                                                                                                                                               >  1:138533/1‑251 (MQ=255)                                                            aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTc                                                                                                                                                                                      >  1:30772/1‑251 (MQ=255)                                                             tcaaGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAGGCCGACCGCAGGAATGGCGGCGAGGAGCATATCCTGCGTCAACGCTAACAGAAATTCGATTACACCCACCAGCGTAGCCCCCAAATCGTCAATGCCCACACTACGCCgcccaaggaagacacagcc                                                                                                                                                                                      >  2:110100/3‑232 (MQ=255)                                                                              atatTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGcc                                                                                                                                                                    >  1:41924/1‑251 (MQ=255)                                                                                atTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGAAGCCAGTGTCAGCAGACTGGCGATCGccc                                                                                                                                                                   >  1:374173/1‑250 (MQ=255)                                                                                   cacaccgtcggacatcaAGTTCAGTCGGGAACCATGTCCTATCGTGCCAAGCAGCGCACACCATCGTAACGCCAGTACGAGAACTTTGAAAACCATCACAAAGCCGACAGCAGTAAGGGCGGCGAGTATTATTTCCTGCGCCAACGCTAACAGAAATTCGATTACCCCCACCCGCGTAGCCTCCACATCGTCAGGGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCCGACTGGCGATCGCCCAGCg                                                                                                                                                               <  2:214601/239‑1 (MQ=255)                                                                                                             gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCCGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGGATTGATGTGGCCtt                                                                                                                                     >  1:283659/1‑251 (MQ=255)                                                                                                                      gACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACatat                                                                                                                            <  1:118171/251‑1 (MQ=255)                                                                                                                       aCCTAACGAGACAAGCAGCGCACACCAGCGTAACGCCCTCACGGGAACGTTTAACACCATCGCAAAGCCGACCGCAGGAATGGCCGCGAGGAGCATATCCTGCGCCAACGCTAACAGAAATTCTATTACCCCCATCCGCGTAGCCCCCAAAGCGTCAGTGCCATCTCGCCGACGACGCAGGTAGCCAGTGTCAGCAGACTGGCTATCGCCCAGCGGGCCAGTCCGGTATTGATGTGGCCTTTAAACATATc                                                                                                                           <  2:126661/251‑1 (MQ=255)                                                                                                                         cTATCGCGCCAAGCAGCACACACCAGCGTAACGCCCGCACGGGAACGTCGCACACCATCGCAAAGACGAAAGCAGGAATGGCGGCGAGGGTCATATCCTGCGCCAACGCTAACAGAAATTCGCTTACAACCATCCGCGTAGCCCCCCAATAGTCAGTGCCATCACTACGCCGCCGCAGGGACCCCGTGGCAGCAGACCGGCCATCGCCCACCGTGCCAGTCCGGTACTGGTGTGGCCTTTacaactaagct                                                                                                                         >  2:363867/1‑241 (MQ=255)                                                                                                                         cTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACata                                                                                                                             <  1:363867/247‑1 (MQ=255)                                                                                                                         cTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGAACGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTAAGCCGACGCAGGTAGCCGGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGAATTGATGTGGCCTTTAAAAATATCgg                                                                                                                         >  1:194274/1‑251 (MQ=255)                                                                                                                           agcGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAATGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGc                                                                                                                        <  1:365349/248‑1 (MQ=255)                                                                                                                           aTCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGc                                                                                                                        <  1:107281/250‑1 (MQ=255)                                                                                                                               aGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACgg                                                                                                                   >  1:21277/1‑251 (MQ=255)                                                                                                                               aGCCAAGCAGAGCAGACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCACAGCCGCCCGCAGGAATGGCGGCGAGGATAATCACCTGCACCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAACCCCCAAATCGTCCGTGCCATCACTACGCCGACGCAGGAAGCCAGTCCCAGCAGACTGGCGATCGCCCAACGTGCCAGCCCGGAATTGATGGGGCCTTTAAACATATCggacactg                                                                                                                   >  2:174839/1‑245 (MQ=255)                                                                                                                                       agcGCACACCAGCGTAACGCCCGCACGGGCACGTTGAACACCATCGCAAAGCCGACCGCAGGACTGGCGGCGAGGAACATAACCTGCGCCAACGCTACCAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGCGCCATCCCTACGCCGCCGCAGGTAACCCGTGTCAGAAGAGTGGCGGTCACACAGAGAGCCATCCCGGACTTGGTGTGGCCTTTAAACagtacggctacggcgtaacac                                                                                                           >  2:327999/1‑231 (MQ=255)                                                                                                                                                cAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAg                                                                                                  <  1:220842/251‑1 (MQ=255)                                                                                                                                                                  ggAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTagcagc                                                                                   >  1:284412/1‑248 (MQ=255)                                                                                                                                                                  ggAACGTTGAACACCATCGCAAAGCCGACCGAAGGAATGGCGGCGAGGAGCAGATCCAGCGCCAACACTAACAGAAATACGATTGCACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGGCGCCGCAGAGAGCCAGAGTCAGCAGACCGGCGGTCGCCAAACGTGACAGACAGATATTGATGGGGTCATTGAACATTGCGGGGACGGCATTAATGAACGGAAAGcctggaaccaggcgcca                                                                                 >  2:284769/1‑235 (MQ=255)                                                                                                                                                                          gaacaccctctcaaactcgtccGCAGGAACGGCGGCGAGGATCATATCCTCCGCCAACGCTAACATAAATTCGATTACCCCCATCCGCGTAGCCACCAATTCGTCAGTGCCATCACTACTCCGACGCTGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTggc                                                                        <  2:276235/231‑1 (MQ=255)                                                                                                                                                                              acCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGcc                                                                    >  1:319054/1‑251 (MQ=255)                                                                                                                                                                                    gCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCACCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACTGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCa                                                              <  1:174839/251‑1 (MQ=255)                                                                                                                                                                                      aaaGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAAt                                                            <  1:120281/251‑1 (MQ=255)                                                                                                                                                                                      aaaGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAAt                                                            >  1:75909/1‑251 (MQ=255)                                                                                                                                                                                                   ggAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTAGATTACAACCATCCGAGTAGCCCCCAAATCGTCAGTGCCATCACTAAGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGGGCCAGGCCGGGATTGAAGGGGCCTTTAAACATATCGGCGACGGCATTAATTAAAGGAAAGCCCGGCTCTAGCCGCAGAACCGTGGAGGCCCTCGCAAAGGGGGGGGTATTg                                               >  2:76551/1‑251 (MQ=255)                                                                                                                                                                                                    gAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGc                                              <  1:95348/251‑1 (MQ=255)                                                                                                                                                                                                              gAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTggg                                    >  1:179976/1‑251 (MQ=255)                                                                                                                                                                                                                   tCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCt                                             <  1:150802/237‑1 (MQ=255)                                                                                                                                                                                                                   tCATATCCTGCGCCAACGATAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATAGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACCTATCGGCGCAGGCATTAATCAACGGGAAGCCCGGCCCTATCCGCAGGACGCTGGGGGCCATGCCAATGGGGGGGGTGTTGcgcggccgggagggcc                               >  2:150802/1‑236 (MQ=255)                                                                                                                                                                                                                             cgcCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCAGCACTAGCTGCAGAACGCTTGCGGCCATCGCAATGGTGGGTGTATTGCTGGAAGTGGGGTGTTGCAAAAGCa                      >  1:337831/1‑250 (MQ=255)                                                                                                                                                                                                                                       aaCAGCAAATCGATTACACCCATCCGCGTGGCCCCCAAACCGTCAGTGCCATCTCTCCGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGTCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCTAAAGCAATCCGGaaa             <  2:292369/249‑1 (MQ=255)                                                                                                                                                                                                                                                 cGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTggcg  <  1:327999/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCG  >  NC_000913/4601845‑4602334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑