Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,567,272 C→A intergenic (‑26/+15) yjiN ← / ← mdtM DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,567,2720CA92.3% 65.4 / NA 26intergenic (‑26/+15)yjiN/mdtMDUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (17/7);  total (19/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACGGTCCCTTTCGGTAACTTATTGGAAAACAGC  >  NC_000913/4567028‑4567518
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       
aTGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATcg                                                                                                                                                                                                                                                   <  1:128937/251‑1 (MQ=255)
        aaaTGATCGGAATCGGCACGCGGCGAAACATCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCTGCAAAAACAGCGTAATGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCaaaaa                                                                                                                                                                                                                                           <  1:362816/251‑1 (MQ=255)
                                  aaCAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTg                                                                                                                                                                                                                 <  1:372874/251‑1 (MQ=255)
                                                   aaaCCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCAcg                                                                                                                                                                                               >  1:168572/1‑251 (MQ=255)
                                                                    cgcCGACCATCGCCGCTTCACCACTCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCAGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGc                                                                                                                                                                              <  1:357430/251‑1 (MQ=255)
                                                                        taCCATCGCCGATTCAGAAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCCAAAACAGCGTAACGAAAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTAGCGCGTCTGAGTTCGATGAGTTTATTCATCACGTTAAGAATCGAGCCTGACGGCCGGATCGCGCAAAACTCACTGCTCCTCCCCTAGCTCGGCTGCATGCTGCTGGC‑ACACGCTATTGAGCaacacagccagg                                                                                                                                                                          >  2:155766/2‑241 (MQ=255)
                                                                                 cgcTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAacg                                                                                                                                                                 <  1:112005/251‑1 (MQ=255)
                                                                                     tCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAacgac                                                                                                                                                               <  1:141988/249‑1 (MQ=255)
                                                                                                        cgcTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGc                                                                                                                                          >  1:80437/1‑251 (MQ=255)
                                                                                                                   aaaTTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGACTAACAGATGa                                                                                                                                >  1:94623/1‑250 (MQ=255)
                                                                                                                       tGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCCAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGAGATTGAGCAATACCGCCAGGGTGAAAACGCCGATAACGCCCGCCACAACGGCTAACAGATGAAACgg                                                                                                                           >  2:333200/1‑251 (MQ=255)
                                                                                                                                                        cagcGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCAc                                                                                                                                                                                                <  1:299636/149‑1 (MQ=255)
                                                                                                                                                        cagcGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCACGCAAAAATCACTGCTCCTCCACTAGCTAGGCTGCCTGATGCTGGC‑GCAc                                                                                                                                                                                                >  2:299636/1‑149 (MQ=255)
                                                                                                                                                             gcgATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGAttt                                                                                     >  1:341719/1‑251 (MQ=255)
                                                                                                                                                                  aaGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGCGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAACAATCACTGCTCCTCCACTAGCTCGGCTGCCTAATGCTGGC‑GCCCGCGATTGAGCACTCCCGCCAGGGTGACAACGACGATAACGCCCGCCACAACGGCCACCAGCTGAAACGGCCAGCTACCGCCGTTAAACCATAGAACGCGGCCGCTTTCgccc                                                                                >  2:173183/1‑248 (MQ=255)
                                                                                                                                                                      aGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTATCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCgaccg                                                                               >  1:23031/1‑248 (MQ=255)
                                                                                                                                                                       gTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGAAGGATCGCGCCAAAATCACTGCTCCACCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGCAAACGACGCTAACGCCCGCCACACCGGCTAACAGCGGAAACGGCACGCGACCGCCGTTAAACCATAGCCAACGGCCGAATTcaccccgcac                                                                           >  2:323750/1‑242 (MQ=255)
                                                                                                                                                                             agagCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACAt                                                                     >  1:24342/1‑251 (MQ=255)
                                                                                                                                                                                                     tGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATAttt                                               >  1:340244/1‑249 (MQ=255)
                                                                                                                                                                                                                  tttATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACg                                >  1:253792/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aTAGAACCTTACGGCAGGGTCGCGAAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GAGCGCGATTGAGCACTCCCACCAGGGTGAAATCAACGATAACGCCCGCCACAACGGCTAACAGATGAAACGACAAGCGACCGCCGTTAAACCATAACAAGCGGCCGATATCAAACGGAAACGACATCACCATCAGGAACACCATATTTAGCGACGCGGAGACgagccatgtcggtcacttat             >  2:175050/1‑232 (MQ=255)
                                                                                                                                                                                                                                       agagCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGACATCCCGCCAGGGTGAAAACGACGCTAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTCAACCCTAGCCAGCGGCCGATTTAGACAGAGACCGACATCACAATCAGGATCACCAAATTTAGCGATGCGGATACGGTCCCTTTCGGCAACTTAttt           >  2:372894/1‑250 (MQ=255)
                                                                                                                                                                                                                                        gagCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACGGTCCCTTTCGGTAACTTATTgg          >  1:299993/1‑251 (MQ=255)
                                                                                                                                                                                                                                         agCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCAcc                                                                 >  1:196156/1‑195 (MQ=255)
                                                                                                                                                                                                                                           ccTGACAGCAGGATGGCGCACAAATCACTGCTACTCCACTAACTCAGCTGCCTGCTGCTGGCACCAC‑CGATTGAGCAATCACGCCAGGGTGAAAACGACGCTAACGCCCGCCACAAAGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCAACGCAAGAGGCCGATTTCGACCGAGCCCGACATCACCAACAGGATCCCCATATTTATCAATGCCGAACCGGTCCATTTCGGAACCTTATAGGaa        >  2:171699/1‑250 (MQ=255)
                                                                                                                                                                                                                                                cGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGCCGGTCCCTTTCGGTAACTTATTGGAAagcagc  >  2:115302/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       
ATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGC‑GCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATTTAGCGATGCGGAGACGGTCCCTTTCGGTAACTTATTGGAAAACAGC  >  NC_000913/4567028‑4567518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑