Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,556,917 A→G E2G (GAA→GGA)  idlP → iraD leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,556,9170AG95.0% 52.6 / ‑4.3 20E2G (GAA→GGA) idlPiraD leader peptide
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (12/7);  total (13/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

TTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCG  >  NC_000913/4556681‑4557160
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   
ttCCCACCGGAATTAAGTATCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTAGCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCaa                                                                                                                                                                                                                                        <  2:290294/250‑1 (MQ=255)
      ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgtg                                                                                                                                                                                                                                 >  1:81363/1‑251 (MQ=255)
                        ggTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGCTGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTAAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGTAAAAAAGGGAATGGCTATGGGAAATGAGCATCATAACAGTGGTGCCCGGTGTGCAggg                                                                                                                                                                                                                >  1:201353/1‑250 (MQ=255)
                                   ttttCATCTACGTAAATATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGGTTTTTATTAAATCATGACTTTTTTTCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGCCTGACATGGATGTAATGCAAAAAAGGGAATGGCTATGGGAAATGAGCATCACTACAGTGGATCACGGTGTTCAGGGCAAGCCgactat                                                                                                                                                                                                    >  2:118809/1‑246 (MQ=255)
                                                                      ataCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGCGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCAGCATCTGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCg                                                                                                                                                                  >  1:182968/1‑250 (MQ=255)
                                                                        aCTAGCGGTTTTTTTTTGAGTTTGATAAATTTTATCAAATCATGAATTTTTTACTGAAAAGTCAGTAGATATTCATAGGCATGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGTAATGAGCATCAATACAATGGTGCACGGTGTTCAAGGCAAGCCGCATATGTTGCTAAACGTCAGGTGTGCGCAAAATGATGAGAc                                                                                                                                                                >  1:309452/1‑250 (MQ=255)
                                                                                        tGAGTTTGCTGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAACTGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGtt                                                                                                                                               >  2:267429/1‑251 (MQ=255)
                                                                                                                cATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACaa                                                                                                                                                              >  1:142700/1‑212 (MQ=255)
                                                                                                                cATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACaa                                                                                                                                                              <  2:142700/212‑1 (MQ=255)
                                                                                                                cATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATaa                                                                                                                        <  1:299203/250‑1 (MQ=255)
                                                                                                                       tattttcTGAAAAGTCAGGTGATATTCATAGGCAATTAAGGTTTTATACTGTGCTGACAGGATTCAGGCCGTTCTCAGACTGAAATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGGGTCCGGTGTTCAGGGCAAGCCTCATATGTTGCTAAACGTCAGGAGTGCGCAAAAGGATGCGACAATCACGTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCa                                                                                                                 <  2:147602/244‑1 (MQ=255)
                                                                                                                                         tAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAg                                                                                                                                                                                                            <  2:64400/141‑1 (MQ=255)
                                                                                                                                         tAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAg                                                                                                                                                                                                            >  1:64400/1‑141 (MQ=255)
                                                                                                                                                        aaGTAAGGTTTTACACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGATTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATctcctc                                                                                >  1:180822/1‑250 (MQ=255)
                                                                                                                                                                 tttATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCttttt                                                                       >  1:339738/1‑250 (MQ=255)
                                                                                                                                                                            cTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCAt                                                           >  1:187897/1‑251 (MQ=255)
                                                                                                                                                                                                ctcAGACTGACATGGATGTAATGCACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCCGGCTGTTTTACCTGAACTTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCAttcct                                       >  2:375159/1‑248 (MQ=255)
                                                                                                                                                                                                                 gTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCg                      <  2:339738/251‑1 (MQ=255)
                                                                                                                                                                                                                           aaaGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGt            <  1:98447/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGCGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGCTGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAACTCGGTATGCAGCGATCTCATCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGCGTGGCAAGTTCATGACCCg  >  2:159476/1‑250 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   
TTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCG  >  NC_000913/4556681‑4557160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: