Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,549,223 T→G V139G (GTG→GGG)  fimH → type 1 fimbriae D‑mannose specific adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,2230TG94.4% 42.7 / ‑3.7 18V139G (GTG→GGG) fimHtype 1 fimbriae D‑mannose specific adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (7/10);  total (7/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCAC  >  NC_000913/4548976‑4549469
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
gTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCTGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGc                                                                                                                                                                                                                                                     <  1:199173/251‑1 (MQ=255)
                  aTCTTTTGCCATAACGATTATACGGAAACCATTACAGACCATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGGAGCTATCCATTTCCTTCCACCACCGAAACACCGCGCGTTGTTTATCATTCGAGAACGGCTAAGCCGTTGCCAGTGGCGCTTTATTTGACGCCTGTTAGCATTGCGGGCGGGGGGGCggtttaagatggggcatt                                                                                                                                                                                                                                   >  2:200343/1‑234 (MQ=255)
                                                                    tcAACGAGGATCGGCTTATGGCTGCGTGGTATCTAATTGTCTCGGGACCGTAAAATAGAGTGGCAGTAGCTATCCATTTCCTACCACCAGTGAAACGCCGCGCGTTGTTTATAAGTCGAGTACGGATAAGCCGTGGCCGGTTGCTCTTTATTTGAAGCCTGTGAGCAGTGCGGGCTGGGGGGCGATTAAATCTGGCTCAGTAATTGCCGTGCTTATGTTGCGACAGACCAACAACTATAACAGCgatga                                                                                                                                                                                   <  2:77216/248‑1 (MQ=255)
                                                                                ggCTTATGGCGGCGTTGTATCTAATTTTGCCGGGACCGTAAAATATAGGGGCAGTAGCTATCCATTTCCTACCACCAGCGAACTGCAGCGCGGTGTTTATAATTCGAGAACGGAGACGCCGTGGCCGGTGGGGCTGTATTTGACTCTTGTGAGCAGTGCGGGCGGGGGTGCGATTAAAGCTGGGTCATTAAGTGCTGTGCTTATTTTGCGACAGACcaa                                                                                                                                                                                                     <  2:34764/219‑1 (MQ=255)
                                                                                                                                   cAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATg                                                                                                                  <  1:214147/251‑1 (MQ=255)
                                                                                                                                   cAGTAGCTAGCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTGTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATGTGACGCCGGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATg                                                                                                                  <  1:364598/251‑1 (MQ=255)
                                                                                                                                            aacagttCCTACCACCAGCGAAACTCCGGGGGGTGTTTATTATTCGAGAACGGATCGGCTGTGGCCGGGGGCGCGTGATTTGTCGCCTGTGGGCCTTGCGGGTGGTGGGTCCATTAAAGCTGGCTCATTAAGTTCCGTGCTTATCTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTTTGGGGCATATTTACTCCAATAATTATGTGCTGGTGCCTTCAGGCGGCTGCGATGTTTCTgata                                                                                                         <  2:298654/246‑4 (MQ=255)
                                                                                                                                              cATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATGCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATGTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCGGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACGATAACAGCGATGATTTCCATTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGt                                                                                                       <  1:378494/251‑1 (MQ=255)
                                                                                                                                                          ccaGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTTCTCGTGATGTCACCGtt                                                                                           <  1:308976/251‑1 (MQ=255)
                                                                                                                                                           caGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTCCGGTGTTTCTACTCCGGTTGTCACCGtt                                                                                           >  2:80264/1‑250 (MQ=255)
                                                                                                                                                                aaaCGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTg                                                                                     >  1:105741/1‑251 (MQ=255)
                                                                                                                                                                            ttgttTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGAAGGCACCGTTAATATGCCCGACTACCCt                                                                         >  1:114095/1‑251 (MQ=255)
                                                                                                                                                                                                 aTAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCtt                                                    >  1:202491/1‑251 (MQ=255)
                                                                                                                                                                                                           gCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGGGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTAtt                                          >  1:277635/1‑251 (MQ=255)
                                                                                                                                                                                                                       ttATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAg                                <  1:15384/249‑1 (MQ=255)
                                                                                                                                                                                                                         aTTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCa                             <  1:356317/250‑1 (MQ=255)
                                                                                                                                                                                                                                        gCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTTTGCGAAAAGCCAACACCTGGGGTATTa              >  1:287730/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 gcgggGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGc    <  1:200343/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggggggGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGcac  <  1:92675/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
GTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTATAACAGCGATGATTTCCAGTTTGTGTGGAATATTTACGCCAATAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCAC  >  NC_000913/4548976‑4549469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑