Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,464	A→T	E54V (GAG→GTG)	fimC →	type 1 fimbriae periplasmic chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,464	0	A	T	97.4%	110.7 / NA	38	E54V (GAG→GTG)	fimC	type 1 fimbriae periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (17/20);  minor base C (1/0);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATAAATC  >  NC_000913/4544228‑4544698                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           cGCCAATGTCCATGCCTGGTTCTCTTTAACCTGTCAGTAATTGTTCAGCATATAATGGGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGTAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCTACTCGCGGAATTTATCCGGCAGTGCAAAAACAAGTGCAACTTGCCGTg                                                                                                                                                                                                                               <  2:321163/250‑1 (MQ=255)      aTGCACAGTCCTGGTTCGTTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATGCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAaat                                                                                                                                                                                                                         <  2:127181/251‑1 (MQ=255)           cAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAatga                                                                                                                                                                                                                    <  1:252606/251‑1 (MQ=255)           cAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAatga                                                                                                                                                                                                                    <  1:292706/251‑1 (MQ=255)               ccTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATTGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGaa                                                                                                                                                                                                                <  1:185099/251‑1 (MQ=255)               ccTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGaa                                                                                                                                                                                                                <  1:244075/251‑1 (MQ=255)               ccTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGaa                                                                                                                                                                                                                <  1:254739/251‑1 (MQ=255)               ccTGGTTCGTTTGAACCAATCAGTAATTGTTTAGCATATAATGTGATATCAGGAACAGGTCAGTGATTAATAAAAACGTCAATGTAAGGGATACGCAGGAAAAAACATGCGGCTTGCTGGCTGGGTA‑CCTGATGTACATGGCAAGGATGGTTGTCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGTGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTGGCCGTGACAAATAATGATGaa                                                                                                                                                                                                                <  2:367306/251‑1 (MQ=255)                    ttCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAAt                                                                                                                                                                                                             >  1:313605/1‑249 (MQ=255)                     tctcTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAAGGATGAAAATAg                                                                                                                                                                                                           <  2:194180/250‑1 (MQ=255)                          ttAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCt                                                                                                                                                                                                      <  1:176547/250‑1 (MQ=255)                               cTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGAAATGATGGTTGACGGACGCGCTGAAGCGGGAGTGGCCTTCGGTGCGACTCGCGTAATTTATCCGGCAGGGCCAAAATAAGTGCACCTAGCCGTGCCAATTAATGATGAAAATAGTAACTAtttc                                                                                                                                                                                                 >  2:110285/1‑249 (MQ=255)                                               agcagATAATGTGATAACAGGAACAGGACAGTGAGTAATAACAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGAATCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGGGGCAAATAATGATGAAAATATAACCTATTTAATTCAATCATGGGTgg                                                                                                                                                                                 >  2:260257/1‑250 (MQ=255)                                                 cagATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGCGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGaa                                                                                                                                                                               >  1:376445/1‑250 (MQ=255)                                                    aTAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATg                                                                                                                                                                           >  1:118754/1‑251 (MQ=255)                                                            aTAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGCGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGgt                                                                                                                                                                   <  2:376445/251‑1 (MQ=255)                                                                        ggaCAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTGCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGt                                                                                                                                                       <  1:231240/251‑1 (MQ=255)                                                                              tGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGtttt                                                                                                                                                 >  1:187814/1‑251 (MQ=255)                                                                                aGTAATAAAAAAGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCAACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGCGCAACTTGCCGTGACCAATAATGATGAAAATAGAACCTATTTAATTCAATCATGGGTGGAAAATGACGATGGGGTAAAGAATGTGCGTTTTAt                                                                                                                                               >  2:291523/1‑251 (MQ=255)                                                                                        aaaCGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAGAGGATGGTCGTTTTATCGTGACGc                                                                                                                                       >  1:245348/1‑251 (MQ=255)                                                                                           cGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGcctc                                                                                                                                    >  1:269666/1‑251 (MQ=255)                                                                                                  gTAATGAAATCGCAAGAAATAGCATTCTGCTTGCTGTC‑ATGTATCCTGATGTTCATGGTAATGATGGTGGCCGGACTCGTTGAAGCGGGAGTGACCTTAGGTGCGACTGGCGTAAATTATCTGGCAGTGCAAAAACAAGTTCAACTTGTCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGGGTAAAGGATGGTCGTTGTATCGTGACTTCTCctctata                                                                                                                             <  2:70382/251‑4 (MQ=255)                                                                                                   tAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGttt                                                                                                                            >  1:252382/1‑251 (MQ=255)                                                                                                         aaTCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATg                                                                                                                      <  1:260257/251‑1 (MQ=255)                                                                                                                         aTTCTGCTTGCTGGC‑AGGAATCCTGATGTTCATGGCAATGATGGTTGACGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGAATCGCGTAATTTATCCGGCAGGGCAAACACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGAACCTATTTAATTCAATCATGGGTGAAAAATGCAGATGGTGTAAAGGATGGTAGTTTTATCGTGACGCCTCCTCTGGTTGCGATGAGGGGAAAAAAag                                                                                                         >  2:355693/1‑248 (MQ=255)                                                                                                                                                cTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGc                                                                                >  1:335682/1‑251 (MQ=255)                                                                                                                                                     gTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGttt                                                                                                                            >  2:173563/1‑202 (MQ=255)                                                                                                                                                     gTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGttt                                                                                                                            <  1:173563/202‑1 (MQ=255)                                                                                                                                                                     gTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTCTCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCcac                                                            >  1:187762/1‑250 (MQ=255)                                                                                                                                                                                      aGCGGGAGTGGCCTTAGGAGCGACTCGCGTAATTTATCAGGCAGGGCAAAAAAAAGTGCAACTTGCCGCGACAAATAATGATGAAAATAGGAATTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCATGACGACTCCTCTGTTTGCGTTGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCCACAAATAAACAATTGCCACAGGACCGGGAAAGTTTAt                                          >  1:179234/1‑251 (MQ=255)                                                                                                                                                                                       gCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTAtt                                         <  2:118754/251‑1 (MQ=255)                                                                                                                                                                                                           gACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGAAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCg                      >  2:145703/1‑250 (MQ=255)                                                                                                                                                                                                                      tttATCCGGCAGGGCAAAAACAAGTGCAGCTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGTATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCt                                                                                                >  2:295991/1‑165 (MQ=255)                                                                                                                                                                                                                      tttATCCGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCt                                                                                                <  1:295991/165‑1 (MQ=255)                                                                                                                                                                                                                           ccGGCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATa      <  1:39292/250‑1 (MQ=255)                                                                                                                                                                                                                              gCAGGGCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATAAATc  <  1:355693/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggCAAAAACAAGTGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGaaa                                                >  1:281226/1‑201 (MQ=255)                                                                                                                                                                                                                                  ggCAAAAACAAGTGCAACTTGCCGTGACAAAGAATGATGAAAATAGTACCTATTTAATTCAACCATGGGTGGAAAATGCCGATGGAGTAAAGGATGGTCGTTTTATCGTTACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACTTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGaaa                                                <  2:281226/201‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           CGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGTCAATGTAAGGAAATCGCAGGAAATAACATTCTGCTTGCTGGC‑AGGTATCCTGATGTTCATGGCAATGATGGTTGCCGGACGCGCTGAAGCGGGAGTGGCCTTAGGTGCGACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATAAATC  >  NC_000913/4544228‑4544698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑