Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,540,111	A→G	intergenic (‑610/‑846)	nanC ← / → fimB	N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,540,111	0	A	G	86.4%	48.1 / 0.9	22	intergenic (‑610/‑846)	nanC/fimB	N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (11/8);  total (13/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

TTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATG  >  NC_000913/4539877‑4540335                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                 ttGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACACCAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTTTCAATAATTTGTTTAGAGTCTAAACa                                                                                                                                                                                                                   >  1:331344/1‑250 (MQ=255)                             cATTGACTTTCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAg                                                                                                                                                                                      <  1:280121/251‑1 (MQ=255)                                tGACTATCTTGGTGCACCTTTATAACTGATATACCAGATAAACAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTATTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAACTAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTACAAGCTAACTCAGTGAGCtt                                                                                                                                                                                   >  1:150206/1‑251 (MQ=255)                                 gACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTg                                                                                                                                                                                  >  1:200176/1‑251 (MQ=255)                                      tCTTGGTGCACCTTTATACCTGTTAAACCAGATCAACAATCAAGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGTGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGAAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGa                                                                                                                                                                              >  1:303400/1‑250 (MQ=255)                                                       accttttcttctaaaacAAAAATCACGCAATTCATACAACAAAATCAGACTTACAATTCGTTTCACAAAATACGTCGATTTTTTTCTAAGAGGAAGATGCCATGTTAAGCCAGACGAACACTTGCGGTGATCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTGAATAGTCTAAACAAGGTGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCATTGTTTAccca                                                                                                                                                             <  2:282301/234‑2 (MQ=255)                                                                     aTCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCa                                                                                                                                              <  1:308311/251‑1 (MQ=255)                                                                                             acaaAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACAAAAGATCTTAGTTTAACTATTTGTTTTAAAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTGGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGttt                                                                                                                      <  1:130509/251‑1 (MQ=255)                                                                                                  aCCAGATTTGCAATTCGTGTCACAAAATATGGCGATCTTTTTCTAAGAGGAAGAGGCCAGGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTgg                                                                                                                 <  1:110908/251‑1 (MQ=255)                                                                                                        tttGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTtgtg                                                                                                           >  1:314873/1‑251 (MQ=255)                                                                                                                        caAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCAc                                                                                           >  1:319476/1‑251 (MQ=255)                                                                                                                                    aTCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTg                                                                                                                                                                               >  1:65476/1‑155 (MQ=255)                                                                                                                                    aTCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTg                                                                                                                                                                               <  2:65476/155‑1 (MQ=255)                                                                                                                                           tcgtagagGAAGATGCCAGGTGAAGCCAGACGAACACTGGCTGTGGTCTTCAAAAGCTAATGATCTGAGTTTAACTTTTTTTTTTATAAAAAATTTGATGATAGGTTAAATAAGGGGATCTTTGCAAGCTAACTCAGTGAGCTTGTTGAAAACCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGTAGTCGATCTGga                                                                         <  1:369732/246‑1 (MQ=255)                                                                                                                                                            aTGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGTTGTTgg                                                       >  1:188770/1‑251 (MQ=255)                                                                                                                                                                       gACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAggg                                            >  1:92022/1‑250 (MQ=255)                                                                                                                                                                                       ggtCTCCAAAAACTAAAGAATTTATTTTAACTATTTTTTTTATAAATAATTTGTTTAGAGCTTAAACAAGGGGAGCTTTGCAAGCTAATTCAGTGAGTTTGGTGAAAATCAGTGTTTCCCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTGGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAgt                            <  1:109048/251‑1 (MQ=255)                                                                                                                                                                                            tcaataattAAAGATCTTAGGTTAATTATTTGTTTTTTAAATAATTTGGTTAGAGTCAAAACAAGGGGAGCTTTGCTAGCTATCTCAGTTAGCTTGGTGAAAATTAGTTTTTCTCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCTCTTAgttt                          <  2:314873/240‑1 (MQ=255)                                                                                                                                                                                                          tCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGTTGTTTCACTTAGTTTGTTTGCCGTATCGCCCg         >  1:312698/1‑251 (MQ=255)                                                                                                                                                                                                              aGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGGCGATCTGGAGAGGCTTGTTGGTGTTGGTGTTTTCAGGATGATGTTTCCATTAGTTTGTTTGCCGTATTGccccggg      >  1:75223/1‑246 (MQ=255)                                                                                                                                                                                                                tttAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCACGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGCGGTTTCAGGATGATGTTTCAATTAGTGTGTTTGCCGTATTGCCCGGCGAAt   >  1:166862/1‑251 (MQ=255)                                                                                                                                                                                                                 ttAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGGTGATGTTTCACTTAGTTTGTTTGGCGTATCGCCCGGCGAATg  >  1:360815/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                 TTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATG  >  NC_000913/4539877‑4540335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑