Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,535,073	A→G	intergenic (‑1020/‑1524)	topAI ← / → ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,535,073	0	A	G	100.0%	79.0 / NA	26	intergenic (‑1020/‑1524)	topAI/ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGCAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACTGTGCACTCCTTGCAGGGGGCGGAAAGGGCGATTGTTCTCTTTTCTCCTGT  >  NC_000913/4534856‑4535319                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       caactctctctcacactATGATCCCAAGTTCGAGCGCGGAATGTAGATGTATGTGACTCGGCGCTGTTGCTATAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGTCGATTTTCCCCGCAATGGGATATTTACATATTTATGGTAGAGGCATTAAGCCAAATGGAGGGCGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCt                                                                                                                                                                                                                       <  2:346795/235‑1 (MQ=255)                                  gcgcGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGGCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTa                                                                                                                                                                                      <  1:254956/251‑1 (MQ=255)                                     cgGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAACAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGCTGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACCTCAGGATGATATAGAGCGCCATTACgg                                                                                                                                                                                   >  1:343899/1‑251 (MQ=255)                                      ggAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACggg                                                                                                                                                                                  <  1:134094/251‑1 (MQ=255)                                                                         tAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTCTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGACACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTATATAAAGTTGATGGGGTTGTGACGcc                                                                                                                                               >  1:171602/1‑251 (MQ=255)                                                                          aaCATCATCGTCTAC‑GTGAACTCATGTTGCTATCACTGTA‑GGTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGCGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTTGTGGCACATATGTATGATATAGAGCGCCTTTACGGGGAACCACTGTACAAATTTGTTGGTGTTGTGACGCCaa                                                                                                                                             <  2:261042/251‑2 (MQ=255)                                                                               catcGACTACTGT‑AACTCACTATGCTATCACTGTAAGTTGCAGCCTAAAAGAGTGATGGAAAAATGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGGGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATATGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCAttt                                                                                                                                           <  2:270131/249‑1 (MQ=255)                                                                                       actGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACg                                                                                                                                                                                    <  1:134080/200‑1 (MQ=255)                                                                                       actGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACg                                                                                                                                                                                    >  2:134080/1‑200 (MQ=255)                                                                                              aCTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAACAAGGACGATTTTCCCCGCAATGGGCTATTTACATATTGATGGTAGAGGCGTGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGACCGCCATTCCGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCAGTTTTCGGCGTAGGTTAATg                                                                                                                           >  2:379544/1‑251 (MQ=255)                                                                                              aCTCACTTTGCTATCACGGTAAGATGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAAAAATAGCAGCATGGCTGGGGGCACATACGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTCGGTTAATg                                                                                                                           >  2:323652/1‑251 (MQ=255)                                                                                              aCTCACGTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATg                                                                                                                           <  1:219358/251‑1 (MQ=255)                                                                                                                    gTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTaa                                                                                                     <  1:284278/251‑1 (MQ=255)                                                                                                                        cAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTg                                                                                                 >  1:367343/1‑251 (MQ=255)                                                                                                                               aaaGAGGGATGGAACAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCCCATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTCCAACGTTGTTGGTGTTGTGACGCCATTTTCGGCGGAGGTTAATGCTCTCAAAATGTCATTGCGTACGCTGGAAAttt                                                                                          >  2:245063/1‑250 (MQ=255)                                                                                                                                             aaaGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAg                                                                            <  1:323652/251‑1 (MQ=255)                                                                                                                                             aaaGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGACAAGACg                                                                              >  1:293343/1‑249 (MQ=255)                                                                                                                                              aaGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCAt                                                                                                                                             <  1:252270/185‑1 (MQ=255)                                                                                                                                                ggACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGc                                                                           >  1:132595/1‑249 (MQ=255)                                                                                                                                                     aTTTTCCCCGCAATGGGATATTTACATATTTATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCCTAGCCCTCTTGAAGCTGAAACAATAACCGCATGGCTGGTGGCACATAATGCTGATATAGAACGCCATTACGGGGAACCACTGACCAAAGTAGTTGGAGTTGTGAAGCCATTTTCGGCGAAGGTTACTGCTTACAAAATGACATTGCGTGAGCTGGCAATTAATgggacagcagagaagggtgt                                                                    >  2:260642/1‑233 (MQ=255)                                                                                                                                                       tttCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCTCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGTAACCACTGTACAAAGTTGTTGGGGTTGTGACGCCTTTGGCGGCGTAGGTTAATGCTATCAAAATGGCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTa                                                                   <  1:218769/250‑1 (MQ=255)                                                                                                                                                       tttCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTa                                                                   <  1:245063/250‑1 (MQ=255)                                                                                                                                                                       tatTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACtgt                                                  >  1:163646/1‑251 (MQ=255)                                                                                                                                                                              atatTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTgggg                                                       <  1:170189/239‑1 (MQ=255)                                                                                                                                                                              atatTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGAATGGCTGGTGGCACATCAGGATGCTATAGCGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTAATTGCGTAAGCTGGAAATTAATGGCAAAGGCGAGCAGGGTTTACTGCCAGTGGGGagctcgggag                                             >  2:170189/1‑240 (MQ=255)                                                                                                                                                                                                                       cATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACTGTGCACTCCTTGCAGGGGGCGGAAAGGGCGATTGTTCTCTTTTCTCCTGt  <  1:46574/251‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       CAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGT‑AACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGCAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACTGTGCACTCCTTGCAGGGGGCGGAAAGGGCGATTGTTCTCTTTTCTCCTGT  >  NC_000913/4534856‑4535319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑