Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,378	G→A	N100N (AAC→AAT)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,378	0	G	A	93.0%	113.6 / 0.2	43	N100N (AAC→AAT)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (17/23);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

CGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCG  >  NC_000913/4516147‑4516626                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         cGCCAGGTCGTGGCTGCCGTGGCCTTTGGTTTCCGTCGCGCTTACGCCAGGGATGCGGTTAAGTACCGCACGCATGGTTGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGTTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTa                                                                                                                                                                                                                                       <  1:219091/251‑1 (MQ=255)               tGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTg                                                                                                                                                                                                                         >  1:285205/1‑251 (MQ=255)                gCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTGCCCAGCGGTTTTACCTGCAGTCCGCTGc                                                                                                                                                                                                                        <  1:83303/251‑1 (MQ=255)                     tGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGTTGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGCTCCCGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCCGTCCCCGACCACGGTCCGGGCATCTTCTTTTGTTGCGGGCGCGGGCCCCCGCGTCCAGCTATTATTTCCCAGCGGTTTTAACTGCCGTCCCCTGCCgccc                                                                                                                                                                                                                   >  2:196775/1‑248 (MQ=255)                       ccGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCGCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCcag                                                                                                                                                                                                                 <  1:324372/251‑1 (MQ=255)                             ttttCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATAACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCCGTCCGCTGCCGTCCAGCAgttt                                                                                                                                                                                                           >  1:14081/1‑250 (MQ=255)                                     cgcgcTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCCCGCGCCACACCCCGCCAGTCGCCGACCACTGTCAGGGCATCTTCTTTTGGAGCGGGCGCGGGTACCAGCGTCCAACTATTATTTCCCAGCGGTTTTACCTGCAGGCCGCTTCCGTCCAGCAGTAGTTGCAGGc                                                                                                                                                                                                   >  2:363475/1‑251 (MQ=255)                                             gatccgggATGCGGTTAAGGACCTCACGCATGGAGGTTGCGTCGGTTTTTGCGAAATCCTCACGGCGGATCACGTCACGCGAGCCAGAATGGGCAAATACGTCGTTTGCACGCGCATCCCCCAGCCAGTCGCCGACCACGGTCAGGGCATCTGCTGTTGGTGCGGGCGCGGGCTCCAGCGTCTAGCGATGATTTCCCAGCGGTTTTTCCTGTAGTCCGCTGCCGTTCAGCAGTTGTTGCAGGCCGCTCTCg                                                                                                                                                                                           <  2:317461/246‑1 (MQ=255)                                                cgtgtctGCGGTGAAGTCCCACACGCATGGTTGTTGTGTCTGTTTTGGCGACATCCTCACGGCGGTGCCCGTTCCGCGCGCCTGCATGTTCAAATACGGCGTTTTCACGCGCATCTCCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTGTTGGTGCGGGCGCGGGCTCCAGGTTCCAGCTATTATTTCCCAGCGGTCTTACCTGCAGTTCGCTGCCGTCCAGCAGTTGTTGCAGTCCGCTCTCGAcg                                                                                                                                                                                        <  2:109141/245‑1 (MQ=255)                                                  gggATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGTGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTTCAGTCCGCTGCCGTCCCGCAGTTGTTGCCGGCCGCTCTCg                                                                                                                                                                                           >  1:317461/1‑246 (MQ=255)                                                  gggATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgtc                                                                                                                                                                                      <  1:196775/251‑1 (MQ=255)                                                    gATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgtcgt                                                                                                                                                                                    >  1:181064/1‑251 (MQ=255)                                                                                             gggCGAAATCCTCGCGGCGGATCACGTCACGCGCGCCAGCATGTCCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTGGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCTTCAgg                                                                                                                                           <  1:280471/250‑1 (MQ=255)                                                                                                  aaaTCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGc                                                                                                                                      <  1:348508/251‑1 (MQ=255)                                                                                                       cTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCCAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGAGCAGGCCGTTGCTCTGCTTGCCACGCGGCAGGCTGGCGTCac                                                                                                                                 >  1:138655/1‑250 (MQ=255)                                                                                                         cACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAc                                                                                                                                <  1:272238/250‑1 (MQ=255)                                                                                                            ggcggATCCCGGCACGCGCGCCAGCATGTTCAAATTCGTCGTTTTCACGCGCATCCCCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCgag                                                                                                                            <  2:83014/251‑1 (MQ=255)                                                                                                                   cacgtcacgCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTa                                                                                                                      <  1:60922/250‑1 (MQ=255)                                                                                                                         ccgcgcgCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGGCAGGCTGGCGTCAACCGAGAGGGTAAATCCGc                                                                                                               <  1:337412/250‑1 (MQ=255)                                                                                                                                    cATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCa                                                                                                    <  1:104149/251‑1 (MQ=255)                                                                                                                                          cAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTg                                                                                               >  1:91083/1‑250 (MQ=255)                                                                                                                                                    gtTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCtt                                                                                    >  1:159483/1‑251 (MQ=255)                                                                                                                                                         cACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCg                                                                               <  1:264843/251‑1 (MQ=255)                                                                                                                                                           cgcgCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAg                                                                             <  1:290175/251‑1 (MQ=255)                                                                                                                                                              gcATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGa                                                                          <  1:281758/251‑1 (MQ=255)                                                                                                                                                                       agccagTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGCGAGGGTAAATCCGCTGTGTGCTGCATTCTGATTGGACGCTTTATCGAGCGATCCCggagg                                                                 >  2:326745/1‑248 (MQ=255)                                                                                                                                                                       agccagTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGc                                                                 <  1:245927/251‑1 (MQ=255)                                                                                                                                                                        gacagTCGCCGACCAGGGTCAGGGCATCTTCTTGTGGGGCGGGCGCGGGTACCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTACGCTGCCGTCCAGCAGTTGTGGCAGGCCGCTCTCGACGTCGGAATCGCCGTGCAGGACGTTGCTCTGCTTGCCGCGCGTCAGTCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCg                                                                <  2:14081/249‑1 (MQ=255)                                                                                                                                                                              cGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGCCGTCGTACTCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCCGCCGCGAGGGTAAATCCGCTGGGTGCGGCATACTGATTGAGAGCTTTATCGAGCGATCCCGGTGCGatctt                                                           >  2:124366/1‑247 (MQ=255)                                                                                                                                                                                      aCGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTgc                                                  <  1:207739/251‑1 (MQ=255)                                                                                                                                                                                                          tGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGTGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGTGCGCTTTATCATGCGATCCCGGTGCGCTATTAACCTGTGCAGCAAAAGCGGAAAACg                                 >  1:5172/1‑248 (MQ=255)                                                                                                                                                                                                            gTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATACCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACgag                               >  1:187528/1‑248 (MQ=255)                                                                                                                                                                                                              gcgggcgcgggcTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGAcc                          <  1:176551/251‑1 (MQ=255)                                                                                                                                                                                                               cgggcgcgggcTCAAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCAGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATTTTAAACTGTGCAGCACAAGCGGAAAGAGAGAGACCg                         >  1:110124/1‑251 (MQ=255)                                                                                                                                                                                                                ggtggcgggcTCCATCGTCTAGTTATTATTTCCCAGGTGTTTTACCTGCGGTCCGCTGCCGTTCAGGAGGTGTTGCAGGCCGCTCCCGACTTCGTAATCGCCGTGCAGGCCGTTGCTCTGCGTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCATTAGCGAGCGATCCCTGTGCGATAGTAACCTGTGCATCAAAATCGGAAATCGAGAGACCgg                        <  2:326442/247‑1 (MQ=255)                                                                                                                                                                                                                            cAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTTAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGgcagc             >  1:192834/1‑250 (MQ=255)                                                                                                                                                                                                                               cGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCTCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgtg                                                                                                           <  1:256249/153‑1 (MQ=255)                                                                                                                                                                                                                               cGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCAAGAGGCAGCAGGc         >  1:251459/1‑251 (MQ=255)                                                                                                                                                                                                                                gTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGATCTCGCCGTCGTAATAGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCATCAGGCTGGCGTCAACAGCGAGGGTAAATGCGCTGTGTGCGGCATACTGATTAAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAGAGCGGAAAACGAGAGACAGGCCAGCGGCAGCAggat        >  1:243279/1‑249 (MQ=255)                                                                                                                                                                                                                                 tCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCcg                                                                                                                                                    >  2:173475/1‑110 (MQ=255)                                                                                                                                                                                                                                 tCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCcg                                                                                                                                                    <  1:173475/110‑1 (MQ=255)                                                                                                                                                                                                                                  ccAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTc       >  1:354997/1‑250 (MQ=255)                                                                                                                                                                                                                                    aGCTATTATCTCCCAGCGGTTTTCCCTGCAGTCCGCTGCCGGCCAGCAGTTGTTGCAGGCCGCGCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAgg    <  2:159483/251‑1 (MQ=255)                                                                                                                                                                                                                                        gTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTGAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCg  <  1:140915/249‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         CGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCG  >  NC_000913/4516147‑4516626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑