Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,514,287 G→C L8V (CTT→GTT)  fecB ← ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,514,2870GC82.5% 82.9 / 15.9 40L8V (CTT→GTT) fecBferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base C (17/16);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

CCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCAGAGC  >  NC_000913/4514055‑4514533
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       
ccgaccgtcttccACGGTGTCAGGTGCGCACGCACTTCGGGCAGGATGCTTGTTGCAGCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTTGCTGATCGCCAGCAGCAGGCCTGCAAAAGCAAAACGGATAAATGCCaa                                                                                                                                                                                                                                       <  2:16121/240‑1 (MQ=255)
 cGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAAc                                                                                                                                                                                                                                      >  1:17140/1‑251 (MQ=255)
                    taGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCACTACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGCTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGCGGCGGAAAAGGCGAGTCTGATCACCAGCAGCACGCCTGCAAAAACAAAACGGATAAATGCCAACTTAATCACATTCCAGCTa                                                                                                                                                                                                                    >  2:5900/2‑250 (MQ=255)
                           gcacgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCATGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCAACAAGcccc                                                                                                                                                                                                            >  1:124392/1‑250 (MQ=255)
                                    tggggcatgacgcggtgtGCATCGCTCTCTTCGGCAAGTCCGATCGGGCTGCCGTCCACGGCGGCCAGCGTTTCGTCGAACGAGAGTTCCAGCACCTACA‑GCCGTTGTGGCGTTGTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGACCACATAATTTCATTCCAGCTTAAAGCCCGGAAAGCCg                                                                                                                                                                                                    <  2:180450/233‑1 (MQ=255)
                                         cAGGATGCGTTTTGCATCGTTATCGTCGGCGATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAGACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGAAAGCCGGGCGtt                                                                                                                                                                                              >  2:156089/1‑251 (MQ=255)
                                          aGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGCTGTGGCGTTTTTCCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAACGGCGTGGCTGATCACCAGCAGCCGGCCTGCAAAAACAAAACGGATAAATGCCCACATAATAAAATTCCAGCTAAAAGCCCGACCAGCCGGGCGtt                                                                                                                                                                                              >  1:54527/1‑250 (MQ=255)
                                                        aTCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCTAAGGCGAGGCTGATCACCAGCAGCAGGCATGCAAAAACAAAACGGATAAATGCCAAAATAATCACATTCCAGCTAAAAAACCGGAAAGACGGGCATTAACACA‑TCaacaatt                                                                                                                                                                              >  2:104000/1‑245 (MQ=255)
                                                               ccgtcgGCAATACCGATCGGGCTTACGTCCACGGCGGCCAGCGCCTCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCTTTTTTGCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTTATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGa                                                                                                                                                                        <  2:299052/249‑1 (MQ=255)
                                                                            cGATAGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGCGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACAGTGGCGGCTACGGCGTGGCGGATCACCAGCAGCAGGCCTGCAAAAACAACACGGATAAATGCCCACATAATCACAATCCCGCTAAAAAGCCGGCAAGCCAGGCGTTAACAAA‑TCAgtaatttaacagaccctgcctaaac                                                                                                                                                           >  2:296910/1‑226 (MQ=255)
                                                                                 gggctGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCaa                                                                                                                                                                                                        <  1:10476/197‑1 (MQ=255)
                                                                                      gACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCgg                                                                                                                                                >  1:138782/1‑251 (MQ=255)
                                                                                            cACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGcc                                                                                                                                          >  1:225547/1‑251 (MQ=255)
                                                                                              cggcggCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGCGCGCGGCTGGCCTg                                                                                                                                        >  2:103006/1‑251 (MQ=255)
                                                                                                  ggcCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCCGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑ACAGAACTTCAACGACCCCTGCATATACAGCGTGcgcgagatcggaagcac                                                                                                                                    >  2:138658/1‑238 (MQ=255)
                                                                                                  ggcCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGcgcg                                                                                                                                                 <  1:138658/238‑1 (MQ=255)
                                                                                                     cAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGAt                                                                                                                                 <  1:103006/251‑1 (MQ=255)
                                                                                                     cAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGGGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGAt                                                                                                                                 <  1:204813/251‑1 (MQ=255)
                                                                                                          cATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAGACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCtt                                                                                                                            <  1:156089/251‑1 (MQ=255)
                                                                                                             cGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgt                                                                                                                         <  1:169810/251‑1 (MQ=255)
                                                                                                               gCGAACGAGAGTTCCAGCACC‑TCAATCCGTTGTGGCTTTTGTGCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgttg                                                                                                                       <  2:88355/251‑1 (MQ=255)
                                                                                                                   aCGAGCGTTCCAGCAAC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGAGTTCGTCCTGAACCGTGGCGGCAAAGGCTTGGCTGATCAACAGCAGCAGGCCTGCAAAAACAAAAGGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCAGGGCGTTCACACA‑TCAGAACTTCAACGACACCTGCCTATAAAGCGGGCGCGACTGGCCGGCATaagaacggaagagagtcg                                                                                                                   >  2:384761/1‑234 (MQ=255)
                                                                                                                   aCGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATa                                                                                                                                    <  1:384761/234‑1 (MQ=255)
                                                                                                                              cagcacgtcaATCAGTTGTGTCGTGTTTTCTAGTGTCAACGTGCCGTGTTCGTCCTGAAACGTGGCGGCAAAGGCGTGTCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAACTGCCAACATAATCACACTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACCCA‑TCAGAACTTCAACGTCCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATTCCTTTGTTGTTGTCGTCATAaga                                                                                                         <  2:116426/243‑1 (MQ=255)
                                                                                                                               gCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACAt                                                                                                                                                                                                                            <  1:318376/135‑1 (MQ=255)
                                                                                                                                 ccc‑acAACCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCAGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGGCGTCATAAGAGCgg                                                                                                     <  1:156830/250‑1 (MQ=255)
                                                                                                                                                tgGCGTTTTTTCGAGGGTAAACGTGCCGTGTTAGTCTTGACCCGTGGCGGCTAAGGAGTGGCTGATCAACAGCAGCCGGCCTGCAAAAACAAAACGGATAACTGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGTCGTTAACAAA‑TCAGAACTTCAACGACCCCTGCATCTACAGCGTGCGCGGCTGGCATGCATAGTTGCCTTTGTTGTTGTCTTCCTAAGAGCgcgtgatgtagacccg                                                                                       >  2:96344/1‑236 (MQ=255)
                                                                                                                                                 ggCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTgg                                                                                      >  1:296006/1‑251 (MQ=255)
                                                                                                                                                             aGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTTATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATTCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGttt                                                                          <  1:41788/251‑1 (MQ=255)
                                                                                                                                                              ttgtAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCCGGCCTGCCAAAACAAAAAGGATAAATGCCAACATAATCACATTCCAGCTCAAATCCCGGCCAGCCTGGCGTTAA‑ACAATCAGAACATCAACAACCCCTGCATATACAGCGTGCGCGGCTGTCCAGCATAGATGCCTTTGTTATTGCAGGCATAAGAGGGGAAGAATGAGTCCAGGACGACGATGtttt                                                                         >  2:92715/2‑251 (MQ=255)
                                                                                                                                                                        gCCGTGGTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑GCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGaa                                                               <  1:112416/251‑1 (MQ=255)
                                                                                                                                                                          cGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCCGCATAGATGCCTTTGTTGTTGTCGTACTAAGAGCGGTTGGAGAAGTCCTGGTCGACGATGGTTTACACCCCGaaat                                                             >  2:275164/1‑249 (MQ=255)
                                                                                                                                                                           gtgtTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGc                                                            >  1:286574/1‑251 (MQ=255)
                                                                                                                                                                                        aCCGTGGCCGCAAATGCGTGGCTGAACACAAGCAGCAGTCGTGCAAAAACAAACCGGACAACTGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGTCGTTAACGCT‑TCTGAGTTTCAACGACCTCTGCATATACAGCGGGAGCGGATTGCCTGCATAGTTGCCTTTGTTGGTGTCGTCATAAGTGCGGATGAAGTAGTCCTTGTCGAAGATGTAGTTCTAACCGAATGCCatttttagatct                                               <  2:91052/251‑12 (MQ=255)
                                                                                                                                                                                                                aTCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATAc                       >  1:338426/1‑251 (MQ=255)
                                                                                                                                                                                                                          gcagGACTGCAAAAACAAAACGGATAAAAGCCAACATAACCACATTCCAGCTAAAAGCTCGGCAAGCCGGGCGTTAACCCA‑TCAGAACGTCAACTACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTg             <  2:332297/251‑1 (MQ=255)
                                                                                                                                                                                                                           cagGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTGACACCGAATGCCAGGTTCAGATATGCCATCTGCGGGCCAACGTCATACGCCACGCGTgc            >  1:283263/1‑251 (MQ=255)
                                                                                                                                                                                                                            agGCCTGCAAAAACCAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCACGGCAAGACGGGAGTTAACACC‑TCAGAACTTAAACGACCACTGCATATACAGAGTGCGCGGCTGGCCTGAATAGATGCCTTTGTTGTTGTGCTCATAAGAGCGGATGAAGGAGTCCTGGTCGAAGATGTATTTGACACCGAATGACCGGTTCGGATCTACAATCTGCGGGCCAAAGTCATACGccccacgtgtg           >  2:176917/1‑242 (MQ=255)
                                                                                                                                                                                                                                   cAAAAACAAAACAGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGAAAGCCGGGCGTTATCACA‑TCAGAACTTCAACGACCCCTGCAAATACAGCTTGCGCGGCTGGCCGGCAGAGATGCCTTTGTGGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTGTTTCACACCGAATGCCAGGTTCAGATCTTCCATCTGCGGGCCAAAGTCATTCGCCACGCGTGCGCCCCaga    <  2:371787/251‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaGAAAACGGATAAATGCCAACATACTCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGTTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCAGAGc  >  1:146717/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       
CCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACA‑TCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGCCCCAGAGC  >  NC_000913/4514055‑4514533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑