Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,514,139	A→C	I57S (ATC→AGC)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,514,139	0	A	C	96.5%	73.8 / NA	29	I57S (ATC→AGC)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (17/11);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAC  >  NC_000913/4513909‑4514380                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGGC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCAAGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAgc                                                                                                                                                                                                                                >  1:185253/1‑251 (MQ=255)               cGGGACTTAAGCAGCAGTACCGGCGCGATTTTCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACgg                                                                                                                                                                                                                  >  1:57389/1‑250 (MQ=255)                    cTTAAGCAGCAGTACCGGCGCGATGTGCTGCAAGGCGGGGAAAACCACCGCCTGGCGACTGCTGTC‑GGCAATGGTACGGACTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTACCGCCGGCATGCCACAGTTACCGGTGCGCACGCACTTCGGGCAGTATGCGGTTTGAATCGTTAACGTCGGACATAACGATCAGGCTGTCGTCCACGGCGGCAAGCGCCTCGGCGAACGagaggt                                                                                                                                                                                                             >  2:298766/1‑249 (MQ=255)                                  ccGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑CCAATc                                                                                                                                                                                              >  1:371787/1‑251 (MQ=255)                                   cGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATcc                                                                                                                                                                                             >  1:299052/1‑251 (MQ=255)                                         gATTTGCTGCAAGGCGATGTAAACCACCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTtgtg                                                                                                                                                                                       >  1:268348/1‑251 (MQ=255)                                           gttgttggtaGGCGCTGTAAACCCCCGCCTGGCGTATGC‑GTCCGTCAATGTTTAGGTCTGGGTGTATAGCGGTAATTGCTGTCAGGCTTGGCGTCGCTCGCGTTCCGACGGACGGCCACTGGTTCAGGTGCTCACGCACTTCGGGCATGATGCGTTTTGCATCGTTATCGTCGGAAATACCGATCGGGCTGCCGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTgg                                                                                                                                                                                      <  2:299488/241‑1 (MQ=255)                                                 gcAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGttttt                                                                                                                                                                               >  1:332297/1‑251 (MQ=255)                                                         aTGTAATCCCCCGCAGGGCTACTGCTGTC‑GGCTATGATCAGGTCTGGTTACAGAGCGGCATTGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑GCAATCCGTTGTGGCGTTTTTTCGAgtgt                                                                                                                                                                       <  2:31664/251‑1 (MQ=255)                                                            tAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGGGTTCCAGCACC‑ACAATCCGGTGTGGGGttttagatcggagggg                                                                                                                                                                    >  2:298606/1‑239 (MQ=255)                                                            tAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGtttt                                                                                                                                                                                <  1:298606/239‑1 (MQ=255)                                                                cccccGCATGGCTACTGCTGTC‑GGCAATGATCATGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCATTACCGATCGGGCTGACGTCCACGGCGGCCAGCGCTTCGGCGAGCGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTGCGAGTGTAAACGTg                                                                                                                                                                <  2:141435/251‑1 (MQ=255)                                                                cccccGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGCGTGTAAACGTg                                                                                                                                                                >  1:244011/1‑251 (MQ=255)                                                                 ccccGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGc                                                                                                                                                               >  1:96307/1‑251 (MQ=255)                                                                           cGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTc                                                                                                                                                     <  1:97194/251‑1 (MQ=255)                                                                                    ggcgtaaaTGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCg                                                                                                                                             <  1:254241/245‑1 (MQ=255)                                                                                                            cAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATcc                                                                                                                     >  1:179498/1‑250 (MQ=255)                                                                                                                            tcctgtctcggcagtccgcgtggtCCGACTGACTGCCACGGTTTCAGGTGAGCTCGCACGTCGGGTAGGATGCATTTTGCATCGTTATCGTCGGCAATACCGAGCGGGCTTACGTCCACGGCGGCCAGCGCATCGGCGAACGATAGTTCCAGCACC‑ACAATCCGTTGTTGCGGTTTTTCGAGTGTAAACGTGCCGCGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCGGAACACCAGCAGCAGGCCt                                                                                                       <  2:67206/226‑1 (MQ=255)                                                                                                                            tCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCTGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCt                                                                                                       <  1:306862/249‑1 (MQ=255)                                                                                                                                                    ccgaccgtcttccACGGTGTCAGGTGCGCACGCACTTCGGGCAGGATGCTTGTTGCAGCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTTGCTGATCGCCAGCAGCAGGCCTGCAAAAGCAAAACGGATAAATGCCaa                                                                             <  2:16121/240‑1 (MQ=255)                                                                                                                                                     cGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAAc                                                                            >  1:17140/1‑251 (MQ=255)                                                                                                                                                                        taGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCACTACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGCTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGCGGCGGAAAAGGCGAGTCTGATCACCAGCAGCACGCCTGCAAAAACAAAACGGATAAATGCCAACTTAATCACATTCCAGCTa                                                          >  2:5900/2‑250 (MQ=255)                                                                                                                                                                               gcacgcacTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCATGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCAACAAGcccc                                                  >  1:124392/1‑250 (MQ=255)                                                                                                                                                                                        tggggcatgacgcggtgtGCATCGCTCTCTTCGGCAAGTCCGATCGGGCTGCCGTCCACGGCGGCCAGCGTTTCGTCGAACGAGAGTTCCAGCACCTACA‑GCCGTTGTGGCGTTGTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGACCACATAATTTCATTCCAGCTTAAAGCCCGGAAAGCCg                                          <  2:180450/233‑1 (MQ=255)                                                                                                                                                                                             cAGGATGCGTTTTGCATCGTTATCGTCGGCGATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAGACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGAAAGCCGGGCGtt                                    >  2:156089/1‑251 (MQ=255)                                                                                                                                                                                              aGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGCTGTGGCGTTTTTCCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAACGGCGTGGCTGATCACCAGCAGCCGGCCTGCAAAAACAAAACGGATAAATGCCCACATAATAAAATTCCAGCTAAAAGCCCGACCAGCCGGGCGtt                                    >  1:54527/1‑250 (MQ=255)                                                                                                                                                                                                            aTCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCTAAGGCGAGGCTGATCACCAGCAGCAGGCATGCAAAAACAAAACGGATAAATGCCAAAATAATCACATTCCAGCTAAAAAACCGGAAAGACGGGCATTAACACATCaacaatt                     >  2:104000/1‑245 (MQ=255)                                                                                                                                                                                                                   ccgtcgGCAATACCGATCGGGCTTACGTCCACGGCGGCCAGCGCCTCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCTTTTTTGCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTTATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGa               <  2:299052/249‑1 (MQ=255)                                                                                                                                                                                                                                cGATAGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGCGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACAGTGGCGGCTACGGCGTGGCGGATCACCAGCAGCAGGCCTGCAAAAACAACACGGATAAATGCCCACATAATCACAATCCCGCTAAAAAGCCGGCAAGCCAGGCGTTAACAAATCAgtaatttaacagaccctgcctaaac  >  2:296910/1‑226 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTC‑GGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACC‑ACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAC  >  NC_000913/4513909‑4514380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑