Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,496,885	G→A	intergenic (+396/‑1387)	leuX → / → insC6	tRNA‑Leu/IS2 insertion element repressor InsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,496,885	0	G	A	100.0%	113.8 / NA	37	intergenic (+396/‑1387)	leuX/insC6	tRNA‑Leu/IS2 insertion element repressor InsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

GGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGGCGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAATCTTTTTGCAGCGCTTGGTGAACGTAATATCGCTGAGTTAAAAACTCGAGATTT  >  NC_000913/4496638‑4497108                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                ggTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGacgc                                                                                                                                                                                                                              >  1:250122/1‑251 (MQ=255)            gCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGTTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCtt                                                                                                                                                                                                                   >  1:234618/1‑251 (MQ=255)                   ccAACAGGGCGGGAATCCGATGGCATTAACAGATATCAAAGTCAGATAAGCCAAGCCAACTGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATTGTTCTACG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGTGGTTGCCAGAGTACGGCGTGACGATACTCGTAAGCTGCTTGCGaaag                                                                                                                                                                                                            <  2:229712/251‑3 (MQ=255)                        agggagggAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTc                                                                                                                                                                                                       <  1:192312/251‑1 (MQ=255)                                   aCGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAAATGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGgaga                                                                                                                                                                                            <  2:22152/251‑1 (MQ=255)                                       gggcaatatcagttatCAAAGTCAGGGCAGCCAAGCCCACTGATAATAAATATTAGCTGATCGATGGTGGCGGTATGCATCGGCTTGTCCATACAAATGGTTCTAAG‑TACTGGTGTTTGCATTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGTGTTTATCCTGAAATTACA‑CTAGCGGATGCCAGAGGACGTCGTGACGAGACGCTTAAGCTTCTTTCGAATGTCGTCGAGCCTGGAGACaaa                                                                                                                                                                                        <  2:242910/238‑1 (MQ=255)                                             tAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGaa                                                                                                                                                                                   >  1:154963/1‑250 (MQ=255)                                                       cAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAgg                                                                                                                                                                        <  1:372603/251‑1 (MQ=255)                                                              agagCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTCCCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAAc                                                                                                                                                                  >  1:274873/1‑250 (MQ=255)                                                                      caagccaaCGGGTAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAACGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTaa                                                                                                                                                           >  1:18433/1‑249 (MQ=255)                                                                         gccaaCGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCAc                                                                                                                                                      >  1:115935/1‑251 (MQ=255)                                                                           caaCGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGaaa                                                                                                                                                                                  >  1:116605/1‑221 (MQ=255)                                                                           caaCGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGaaa                                                                                                                                                                                  <  2:116605/221‑1 (MQ=255)                                                                             aCGGATAAGCAATATTAGCTGACTGATGGTGTCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAAc                                                                                                                                                  <  1:357217/251‑1 (MQ=255)                                                                              cGGATAAGCAATATTAGCTGACTGATGGTGGCGGTTTGAATCTGCTTGGCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGATGGCACGTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGGCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGGCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAAcc                                                                                                                                                 <  2:234618/251‑1 (MQ=255)                                                                                gATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCtt                                                                                                                                               >  1:164761/1‑251 (MQ=255)                                                                                  aaaGCAATAATAGCTGACTGAGGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTGTCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACATGGTGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGTGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTTAAGCACGAACCTTTa                                                                                                                                             <  2:154963/250‑1 (MQ=255)                                                                                             aGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTcgc                                                                                                                                   <  1:298496/250‑1 (MQ=255)                                                                                                     gaTGGTTGCGGTATGCATCTGTTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTTGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCTGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGtg                                                                                                                          <  1:67743/251‑1 (MQ=255)                                                                                                        ggtggCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGtggca                                                                                                                       <  1:293549/251‑1 (MQ=255)                                                                                                                         gCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGGACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAgg                                                                                                      >  1:148197/1‑250 (MQ=255)                                                                                                                             gtccagtcaAATGGTTCTAAGGTA‑TGGCGTTTGCATGAACGTTATGAGGGAAAGCAAAAAATGAGGACCCTTGTGGTGTATCCTGAAATC‑CAGCTAGCGGATGCCAGAGAACGTCGTTACGAGACGCGTAAGCCGCTTGCGAATGGCGCCGATCCGGGAGACATAAAGAAAAATGAGAAGGTTGAAAATAGTAAAGCACGAAACTTTTAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGta                                                                                                  <  2:36111/244‑2 (MQ=255)                                                                                                                                         ggTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCATGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGAAGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGAGGCATGGCACCAATAAAAAGTGGTCTGAAGATAACGc                                                                                      >  1:347524/1‑251 (MQ=255)                                                                                                                                                      tGGCGTTTGCAGAACCGTTGTGAGGGAAAGCAAAAAATGCAGGCAATTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGCCGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATACGGGAGACAAAAAGACAACTGATAAGGTTGAACAGAGTAAAGCACGAATCTTTCAAGACGTCGCGATTGTGTGGAATGGCACCAATAAATAGGAGACTGCAGATCACTCCCATCGtgtgtt                                                                          >  2:170553/1‑249 (MQ=255)                                                                                                                                                                  tACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTACAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTg                                                               >  1:126689/1‑250 (MQ=255)                                                                                                                                                                    ccGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAg                                                            <  2:115935/251‑1 (MQ=255)                                                                                                                                                                     cGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGATAAAAAGTCTTGa                                                              >  1:260729/1‑248 (MQ=255)                                                                                                                                                                        tATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATa                                                         <  1:335424/250‑1 (MQ=255)                                                                                                                                                                         aTGAGGGAAAGCAAAAAAGGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAAt                                                       <  1:244805/251‑1 (MQ=255)                                                                                                                                                                                              tGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGACAAATGATAAGGTTGaa                                                                                                                                                                   >  2:190851/1‑122 (MQ=255)                                                                                                                                                                                              tGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGaa                                                                                                                                                                   <  1:190851/122‑1 (MQ=255)                                                                                                                                                                                                gCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCGTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAATCTTTTTGCAGCGCTTGGTGAACg                                <  1:230719/251‑1 (MQ=255)                                                                                                                                                                                                       gggTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATaa                                                                                                           <  1:298591/169‑1 (MQ=255)                                                                                                                                                                                                       gggTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATaa                                                                                                           >  2:298591/1‑169 (MQ=255)                                                                                                                                                                                                         gTTTATCCTGAAATCACA‑CTAGCGGATGCAAGAGTACGTCGTGCCGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAATCTTTTTGCAGCGCTTGGTGAACGTAATATCGc                       >  1:131929/1‑251 (MQ=255)                                                                                                                                                                                                             aTCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGACGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAATCTTTTTGCAGCGCTTGGTGAACGTAATATCGCTGa                    <  1:97748/250‑1 (MQ=255)                                                                                                                                                                                                                               gcgtaTGCCAGAGTACGTCGGTACGAGACGCGGAAGCTGCTTGCGAATGGCGACGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAAGCTTTTTGCAGCGCTTGGTGAACGTAATATCGCTGAGTTAAAAACTCGAGAttt  <  2:164761/247‑1 (MQ=255)                                                                                                                                                                                                                                cGGATGCCAGAGTACGTCGTGACGCGACGCGTAAGATGCTTGCGAATGGCGACGATCCGGGCGACAAAAAGAAAAATGATAAGGATGAAAAGAGAAAAGCACGAACCTTTAAAGAAGTCGCGATTGCGAGGCATGGCACCAATAAAAAGTGGTCAGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGACGAGAATCTTTTTGCAGCGCTTAGTGAAAGTAATATCGCTGAGTTAAAAACTCgagaatt  >  2:221346/1‑247 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                GGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAG‑TACTGGCGTTTGCAGTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACA‑CTAGCGGATGCCAGAGTACGTCGTGACGAGGCGCGTAAGCTGCTTGCGAATGGCGTCGATCCGGGAGACAAAAAGAAAAATGATAAGGTTGAACAGAGTAAAGCACGAACCTTTAAAGAAGTCGCGATTGAGTGGCATGGCACCAATAAAAAGTGGTCTGAAGATCACGCCCATCGTGTGCTAAAAAGTCTTGAAGATAATCTTTTTGCAGCGCTTGGTGAACGTAATATCGCTGAGTTAAAAACTCGAGATTT  >  NC_000913/4496638‑4497108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑