Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,492,470	C→A	G19V (GGC→GTC)	idnT ←	L‑idonate/5‑ketogluconate/gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,492,470	0	C	A	100.0%	69.7 / NA	27	G19V (GGC→GTC)	idnT	L‑idonate/5‑ketogluconate/gluconate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/14);  total (13/14)

GCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GG‑CTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCG  >  NC_000913/4492227‑4492710                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     gcccttggccctctttttttACCAAATGCATCAATCAGGGTAGGGGCGATCCTTTGGGCCGCACCCGTATCAGAAAGCAGCTTGCCGACTATGGCCCCGAAACCGAGGATCATGGCCGGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGTACTGCCTGTGCATCCATCCTGTCGGCAAATCCGACGACGGCAGTTACCAGGACGAGTGCAATAAAGCCTTTAACGTTAAAGACGatcat                                                                                                                                                                                                                                               <  2:183034/237‑1 (MQ=255) gcCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGatcat                                                                                                                                                                                                                                               <  1:140543/251‑1 (MQ=255)  cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGAAGGCCTGGGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGatcatc                                                                                                                                                                                                                                              >  1:353430/1‑251 (MQ=255)        gCACGCGTGTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAGTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGAt                                                                                                                                                                                                                                         <  1:63757/250‑1 (MQ=255)                    tACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGGGCCGCACCCGTATCAAAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGAAGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAgcgc                                                                                                                                                                                                                             <  2:115534/250‑1 (MQ=255)                                       cGTAGTGGCGCTACGTTGGGCCGCAAACGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGCATCATTGCCAGCACGCAGAACGTGCTGCCGTTACCATTTTGTATAGAGCGCCGGAAGGCCTGTGAATCCATCGCTTCGGCAAATCCGACGACAGCAGCTACCAGAAAGAGGGAAATAAAGACGCTAACTTTAAAGACGATAATCCGGATCAGCAGCATCGCGACgaccgacgccataata                                                                                                                                                                                                          >  2:163545/1‑235 (MQ=255)                                        gTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGAt                                                                                                                                                                                                        >  1:377663/1‑251 (MQ=255)                                                                                    gCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGGTCATCAGATTAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGACTTTATCTCTTATTAGTGCCAGAAAAGCAgcg                                                                                                                                                            >  2:300267/1‑251 (MQ=255)                                                                                                   gAAACCGAGGATCATTGCCAGACCGCCGAGCGTGATGCCGATAAAATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGTCAGCTACCAGTACGAGGGCAATAAAGCAGTTAACTTTAAAGACGCTCATCAGGATCAGAAGCAGCGCGCCGCCTGCCGCAATACTGATTAATGGCATCGTCTTTATACCTTATTAGTGACAGAAAAGGAGCGATTTAGCGCTGCACg                                                                                                                                             >  2:301289/1‑251 (MQ=255)                                                                                                            gATCATTCCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTTTGCACCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑Aggg‑g‑ggg                                                                                                                                 <  1:227697/251‑2 (MQ=255)                                                                                                                     ccgccctccgagctagcggcCGATACCAGTTTGTATAGAGTGCAGGTCGGCCTGTGCATCCACCCCTTCGGCAATCCCGCCGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTATCTTTAAAGACGATCATCAGGATAAGAAGCAGCGCTCCGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCTCTGCACGT‑AGGG‑GTGG‑CTGTTAAAc                                                                                                                        <  1:69475/233‑1 (MQ=255)                                                                                                                               gggttgcggccgtttccATTTTGTATAGAGTGCAGGACGGCTTGTGCATCCACCCCTTCGGCAAACCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCAGAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAAc                                                                                                              <  1:57905/236‑1 (MQ=255)                                                                                                                                             aCCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATcaac                                                                                                >  1:108672/1‑251 (MQ=255)                                                                                                                                                         agagTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTg                                                                                    >  1:9578/1‑251 (MQ=255)                                                                                                                                                            gTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTCCCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGcc                                                                                 <  1:57355/251‑1 (MQ=255)                                                                                                                                                             tGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCg                                                                                >  1:304685/1‑251 (MQ=255)                                                                                                                                                              gCAGTACGGCCTGTGCATCCATCCCTCCGGCAAATCCGACGACGGCAGCAACCAGAACGAGGGCAATAAATCCGTTAACTTTAAAGACGATCATCAGGATAAAAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCTCCATCAACAAACAACAGGTGGCCg                                                                                <  1:320914/250‑1 (MQ=255)                                                                                                                                                                        cTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATACAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGGAG‑GG‑CTGTTACACAGCCACTAACATGCCGCCCTCAACAAACAACAGGTGGCCGTTTACGAAATc                                                                     >  2:100832/1‑251 (MQ=255)                                                                                                                                                                                ccatccCTTCGGCAAATCCGCCGACGGCAGCTACCAGAAAGAGGGCAAAAAAGCCGTTAACTTTAAAGACGATCATCAGTATAAGAAGCAGCGCGCCGCCTGCCGCAATAAGGATTAATGGCATAGTCTTTTTCGCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCAGCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCtt                                                             <  1:133759/251‑1 (MQ=255)                                                                                                                                                                                          ggaaaaTCCGACTACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAATTTTAAAGACGATAATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAg                                                   <  1:100832/248‑1 (MQ=255)                                                                                                                                                                                             aaacccGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAAAAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGaa                                                <  1:309426/247‑1 (MQ=255)                                                                                                                                                                                              aaTCCGACGACGGCAGCTACAAGAACGATGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGAGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTTGGGG‑G‑GG‑CTGTTAAACAGACACTAACATGCCGCCATCAACAAAAAACAGGTGGCCGTTTACGAAATCAGGGGCTTTTGAAGAAACGAac                                               >  2:76358/1‑251 (MQ=255)                                                                                                                                                                                                              cTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATc                                >  1:64987/1‑250 (MQ=255)                                                                                                                                                                                                                              aaTAAAGCCGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTcc               >  1:150362/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGCCTCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAATCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACTCCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTg       <  1:254953/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGTTAACTTTAAAGACGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTg       >  1:46640/1‑251 (MQ=255)                                                                                                                                                                                                                                           aCTTTAAAGACGATCATCAGGATAAGAAGCCGCGCGCCGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTgcggcg  <  1:302098/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACGAGGGCAATAAAGCCGTTAACTTTAAAGCCGATCATCAGGATAAGAAGCAGCGCGACGCCTGCCGCAATAATGATTAATGGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGT‑AGGG‑G‑GG‑CTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCG  >  NC_000913/4492227‑4492710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑