Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,473,9000CT93.5% 72.6 / ‑1.5 32G52R (GGA→AGA) bdcAc‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (16/13);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

CACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCG  >  NC_000913/4473655‑4474146
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
cACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGGATTTACCACCAGGATATCCACTGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGtct                                                                                                                                                                                                                                                   >  2:85951/1‑251 (MQ=255)
   ggAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCtt                                                                                                                                                                                                                                                <  1:52446/251‑1 (MQ=255)
     aGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTtgt                                                                                                                                                                                                                                              <  1:173567/251‑1 (MQ=255)
            ggttaagagtaccccgccTTCTGGCATCTGCCCGGCTGCTCGAACAGAGGCATGATAAGGTGCATGAATATTGATTTTGTAAAGGCGAACAATATCGTCGTCATTTAAGTCCAGTGCCTCGCCTAATACGCCAATCCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACTGCGTCTCTGTCAGCTCTATCTGTGAATACTGCTGTCGCTCTTGTCTTTTGTGCCAgg                                                                                                                                                                                                                                        <  2:155996/238‑1 (MQ=255)
                     gcgcCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCgg                                                                                                                                                                                                                              >  1:294497/1‑251 (MQ=255)
                       gcccgccTTCAGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGc                                                                                                                                                                                                                             >  1:91254/1‑250 (MQ=255)
                               tCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCg                                                                                                                                                                                                                     <  1:110127/250‑1 (MQ=255)
                                         gCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAg                                                                                                                                                                                                          <  1:76668/251‑1 (MQ=255)
                                                ggcTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATAAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCACTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTCTCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCAACCCCGCATGGGTgtctc                                                                                                                                                                                                   >  2:185/1‑247 (MQ=255)
                                                     aaactgagtcattaTAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGTCATTTAATTCCAGGGCCTAGCCAAAGACTCCACTATCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACTAACGACT‑TCAATGACAGCGTCTCTGTCAGCCCTATCTGTGAATTCTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTATCGGCATCTTTTGACCCCGCATAGGTGAATCGTACa                                                                                                                                                                                              <  2:275200/238‑1 (MQ=255)
                                                                tGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTAAAGGGCCTCGCCAAAGACGCCAATACCTGCATTTAACACCAGGATATCGAATGCGCAGCTCTTACAAACGACA‑TCAATGACAGCGTCACTGTCAGCACTATCTGTGACTACTGCTGTCGCTCTATTCTCTCGTACTAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCagc                                                                                                                                                                                   >  2:34260/1‑249 (MQ=255)
                                                                 gATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCg                                                                                                                                                                                  <  1:137592/251‑1 (MQ=255)
                                                                    aaGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGGATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                                                                                               <  1:85951/251‑1 (MQ=255)
                                                                    aaGGAACATGAATATTGATTTAGAAACGGCGATCAATATCGTCGGCATTTAATTACAGGGCATCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCCATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTAGTGCCCGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGAGAATCGTACATTGGCCCCATccga                                                                                                                                                                                >  2:139454/1‑247 (MQ=255)
                                                                                            aaaGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGCCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTGAGCGGCATCTTTCGACCCCGCCTAGGCGAATCGTACCTTCGCACCATCGGTGACGACCCGACGTAAGATcgcgacc                                                                                                                                                       >  2:344993/1‑248 (MQ=255)
                                                                                                     cAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTAACACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGAGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATAccc                                                                                                                                              >  1:327667/1‑250 (MQ=255)
                                                                                                                     tAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAgg                                                                                                                               >  1:124609/1‑250 (MQ=255)
                                                                                                                          ccAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATg                                                                                                                            >  1:30910/1‑248 (MQ=255)
                                                                                                                            aGGGACTCGCCAAAGGCGCCAATACCGGCATTTACAACCAGGATATCCAATGCGCCGCTCTTACGAAAGACA‑TCAATGACAGCGTCTCTGTCAGAACTATCTGTGACTACTGCTGTCGCTCTAGTCTCTTGTGCAAGGAGTTTAGTGGCTTCTTTAGACCCCGCATAGGGGAATCGTACATAGGCCATATCGGGGCCGCCACGAAGTACGATAGCGGCACCGATAAAACGACTGCAAACGAGGATGAGaa                                                                                                                        >  2:47011/1‑250 (MQ=255)
                                                                                                                             gggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGaa                                                                                                                                                                                                     <  1:255235/172‑1 (MQ=255)
                                                                                                                                            cGCCAATTCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTaaaa                                                                                                       <  2:91254/251‑1 (MQ=255)
                                                                                                                                               cAATCCCTTCATTTACCTCCATGATATCCAACTCGCTGATCTTACGAACGACACTCA‑TTACAGCGTCCCTTTCAGTACTATCGGTGAATTCTGCTGTCGCTCTTGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAAGCGTACATTGGTCCCATCGGTGACGAAACGACGTACGATAGCGTCACAGATACCACGACTGCCACCGAGGATTAGAACTGTCTTATCTGTAAAAgcg                                                                                                    <  2:308763/251‑1 (MQ=255)
                                                                                                                                               cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCATTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATAGAGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGGCTTACCTGTaaaaacg                                                                                                    >  2:309911/1‑248 (MQ=255)
                                                                                                                                                             accaacAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTATAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGAATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGTCGTACGATATCGGCACCGATACCACGACTGACACAGAGGATGAGACGTGTCTTACCTGTAACAGCGCCCATAAGGACTcc                                                                                      >  2:6829/1‑251 (MQ=255)
                                                                                                                                                                            aaTGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGACTCTTGTGCCCGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGGGAATCGTACATTGGCCCCATCGGTGACGACACGACGTACGATCGCGGCACCGATACCACGACTGCCACCGCGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGCCTCCTTGGTTTATTATGt                                                                        >  2:169774/1‑250 (MQ=255)
                                                                                                                                                                                           cgaactaCA‑TCAATGACTGCGTCCCGGTCAGCACTATCTGGTAAGCCTGCTGTCGCTCTATTCTCTTGTGCCAGGCGTTTCGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGGTGACGAAACGACGTACGATAGCGTCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACaaaa                                                        <  2:359599/251‑1 (MQ=255)
                                                                                                                                                                                             aacgaCA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATCGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACt                                                      >  1:55632/1‑251 (MQ=255)
                                                                                                                                                                                                gaCA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGtt                                                   >  1:83742/1‑251 (MQ=255)
                                                                                                                                                                                                                  ctctGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAAcc                                  >  1:197301/1‑250 (MQ=255)
                                                                                                                                                                                                                      gTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAgg                                <  1:185/249‑1 (MQ=255)
                                                                                                                                                                                                                          ccACTAACTGTGAATCCTGCTGTCGCGCTAGTCTCTTGTGCCAGGCGGTTGGCGGCATCTTTCGACCTCGCATAGGTTAATCGTACATTGGGCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGATGATGAGAACTGTCTTTCCTGTAAAAGCGCCCATAAGGACTCCTTTATTTATTATGTAACATGCATTACAAATCTGTTTTAACTTTCTGGCAACAGGTTTTGt                          <  2:33971/250‑1 (MQ=255)
                                                                                                                                                                                                                                  tgtgAATACTGCTGTCGATCATGGCTAGTTTGCCAGTCGTTTAGTGGCAGCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAGCGGTGACGAAACGACGTACGATAGCGGCACCGTTACCACGACTGCCACCGAGGATGAGAACTGTCTAACCTGTAAAAGCGCCCATAAGTACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTc                   <  2:290767/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccAGTCTCTTGTGCCAGGCGTTTGGCGGCATCTTTCGACCCCGCATAGGGGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGTACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTcg  <  2:362876/248‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
CACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACA‑TCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCG  >  NC_000913/4473655‑4474146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑