Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,304	C→T	intergenic (+69/‑143)	ytfP → / → chpS	gamma‑glutamylamine cyclotransferase family protein YtfP/ChpS antitoxin of the ChpB‑ChpS toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,304	0	C	T	100.0%	81.4 / NA	27	intergenic (+69/‑143)	ytfP/chpS	gamma‑glutamylamine cyclotransferase family protein YtfP/ChpS antitoxin of the ChpB‑ChpS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/14);  total (13/14)

ATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGG  >  NC_000913/4448060‑4448543                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                aTCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCAc                                                                                                                                                                                                                                           <  1:270098/251‑1 (MQ=255)     tATTGACAACGCCAAGCTGTCCGAACTTTATGCCTTGCGCACCAGGGGCGGTGAATGCGCGGTCCAGTTGATTCAGTCGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATTCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCtt                                                                                                                                                                                                                                        <  2:181527/250‑1 (MQ=255)          aCAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATTGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCtt                                                                                                                                                                                                                                  <  1:32312/251‑1 (MQ=255)             aCGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTtc                                                                                                                                                                                                                               <  1:90531/251‑1 (MQ=255)              cGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCCGACGCCGTACGGGAGTGCATGGATGTACGGTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAAGGATAAGTAACCCTATTCATACGCCACCTTCGGGAGGCGTTGTTTTTTGCGGAACGACTCGCCTTCTGTTTTGTAATTTCCTCACCTTTTTCttttat                                                                                                                                                                                                                              >  2:296522/1‑249 (MQ=255)                           gAACTTGATGCCTTGCTCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTGTTGTAATTTCCTCTCCTTTTGCTTTTCTCGCCGAGCCGCtt                                                                                                                                                                                                                 <  1:214857/251‑1 (MQ=255)                           gAACTTGAAGCCGTGCGCCCCAGGGGCGTTGAATACGAGCGACAGTTGTTTCAGACGCGGTACGGGAGTGCAGGGATGTACGTGTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTGTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCtt                                                                                                                                                                                                                 <  2:250307/251‑1 (MQ=255)                             actagatgccgcgctcACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGCCCCGTCGATGGATTAAAGCTAATGGAAAGCGCCGACTGGTTAGACAGGGATAAGTATCTATAGGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGTCTCGCATTCTGTTTTGGAATTTCCTCCCCTTTTGCTTTTCTCCCCGAGCCGCTTTc                                                                                                                                                                                                               <  1:16063/236‑1 (MQ=255)                                                                  caccAGATGAATCAGATGCCGTACGGGAGTGCACGGATGAAAGATTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAACGACGACTGGTTAGACAGGGATTAGTAACCATATGCATAAAACACCTTAGGGTGGCGTTGTTTTTTGCAGGAAGACTCGCATTCTGCTTTGTAATTTCCTCACCTTTTGCTATTCTCTCCGTGCCGCTTTCCATATCTATTACCCCCTAAAAAActctttactcattc                                                                                                                                                                          >  2:45673/3‑241 (MQ=255)                                                                                    ccGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTaa                                                                                                                                                        <  1:320266/251‑1 (MQ=255)                                                                                       tACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTCATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAAc                                                                                                                                                     >  1:367000/1‑251 (MQ=255)                                                                                                                aTCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTtat                                                                                                                             >  1:265944/1‑250 (MQ=255)                                                                                                                                   aTTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAagg                                                                                                           >  1:336414/1‑249 (MQ=255)                                                                                                                                           tAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGc                                                                                                     >  1:137657/1‑247 (MQ=255)                                                                                                                                                gAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAActct                                                                                                                                                                                    <  1:185307/163‑1 (MQ=255)                                                                                                                                                gAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGt                                                                                             >  1:343942/1‑250 (MQ=255)                                                                                                                                                       gcgACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCAt                                                                                     >  1:117882/1‑251 (MQ=255)                                                                                                                                                             ggTTAGACAGGGATAAGTAACCATATGCCTACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATaaaa                                                                                 >  1:313946/1‑249 (MQ=255)                                                                                                                                                                 aGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATg                                                                            <  1:299669/250‑1 (MQ=255)                                                                                                                                                                               taCCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAg                                                              <  1:268164/249‑1 (MQ=255)                                                                                                                                                                               aaCCATATGCATACCCCCCCTTGGGGTGGCGTTGTTTTTTGCAAGACGACTCGCATTTTGTTTGTTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAg                                                              <  2:313946/250‑1 (MQ=255)                                                                                                                                                                                               cACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAat                                             <  1:146057/251‑1 (MQ=255)                                                                                                                                                                                                         tGGCGGTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATg                                    >  1:58649/1‑250 (MQ=255)                                                                                                                                                                                                                  tttttGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTACCAATATCGTAATGCAAGaactt                           >  1:133159/1‑250 (MQ=255)                                                                                                                                                                                                                    tttGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaacttaac                        >  1:180496/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGc      >  1:97684/1‑251 (MQ=255)                                                                                                                                                                                                                                          gttgtGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTgg  <  1:316110/247‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGG  >  NC_000913/4448060‑4448543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑