Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,440,768	G→A	L150L (CTG→TTG)	paeA ←	putative cadaverine/putrescine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,440,768	0	G	A	86.8%	81.0 / 6.1	38	L150L (CTG→TTG)	paeA	putative cadaverine/putrescine exporter
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (14/19);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-02

GATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCGCC  >  NC_000913/4440537‑4441012                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gaTCAACCCAAATCACGTTTTCACGCGGTGACAGTGAAGACGGAACGGTACGGTATTCCATCTCAAAGACGTTTTCAATCTGCTCGTTTTCCTGTTTACTTAACTCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAgg                                                                                                                                                                                                                                     <  1:232122/251‑1 (MQ=255)     aaaCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCGCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGa                                                                                                                                                                                                                                 <  1:33405/251‑1 (MQ=255)                 gTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGAGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCAAGGCCGTTGAAGAACCACACCAACGGGGTGCAACCGTACAGGCAGAAACGCATCGGGt                                                                                                                                                                                                                     >  2:122475/1‑251 (MQ=255)                          cggggtCATTGAAGACGGATCGGTACGAGATTCCAGCACAAAGACTTTTTCAATCAGCTCGTGTTCCTGTTTCCGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑c                                                                                                                                                                                                           <  1:44872/247‑1 (MQ=255)                               tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTACCCCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGCAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGCAGAACCAACCCAACGGGGTGCAAACGTACAGGCAGAAGGGCATCCGGTTAATGATA‑Cgaaa                                                                                                                                                                                                       >  2:283469/1‑247 (MQ=255)                                           gAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAACTACGGAAGCTTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAACGCATCGGGTTGATTATA‑CGCACAGCCACAGCttat                                                                                                                                                                                          >  2:214590/1‑249 (MQ=255)                                            aaCGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTACCACGCCCGCCAGCGCACCGTCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTCCCATTGGCAGTTTGAAAATACGGACGCTTATGTTCGCCAGGCCGTTGCAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATG‑TATCGCAAAGCCACAGCTTCTg                                                                                                                                                                                         >  2:103167/1‑251 (MQ=255)                                                        ttCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGGGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACAACACCAACGGGGAGCAAACGTACAGGCAGAAGCGCATCGGGGTGATGATC‑CGCCAGGCAACAGCTTCTGGCGCAA‑TCATAc                                                                                                                                                                            >  2:74900/1‑251 (MQ=255)                                                               tCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCg                                                                                                                                                                     >  1:338349/1‑251 (MQ=255)                                                                  aaGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGttt                                                                                                                                                                  <  1:257622/251‑1 (MQ=255)                                                                          ttcaatcactgtgtgtCCCTGTTTACTTAACCCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTTCCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGtt                                                                                                                                                           <  2:187408/238‑1 (MQ=255)                                                                             aaccAGCCCGTGTTCCTGTTTACGTACCCCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAAt                                                                                                                                                       <  1:74900/248‑1 (MQ=255)                                                                              aTCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCTCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGTTGCGTTTCGGGGTTAAATc                                                                                                                                                      >  1:80910/1‑251 (MQ=255)                                                                                cAGCTCTTGTTCCTTTTTACGTAACCCACCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCg                                                                                                                                                    <  1:112523/251‑1 (MQ=255)                                                                                     cgttctCCTGTTTACGTAACCCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCTCAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAAc                                                                                                                                               <  1:338123/246‑1 (MQ=255)                                                                                     cGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCaaa                                                                                                                                                <  1:76871/250‑1 (MQ=255)                                                                                                  actgaccacctccggcAGCGCACCGGTTTCCACTACCGCGGAGATGTCATCAGAATTGATGTCAGCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑GGCAAAGCCACAGCTGCTGGCGCAA‑TCATTCCGATGCTTTTCGGGGTTAAATACGCAAACAGGATAAACATg                                                                                                                                   <  2:341385/235‑1 (MQ=255)                                                                                                                             ttCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAAt                                                                                                                                                       <  1:22920/203‑1 (MQ=255)                                                                                                                             ttCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAAt                                                                                                                                                       >  2:22920/1‑203 (MQ=255)                                                                                                                             ttCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAAt                                                                                                                                                       <  1:126382/203‑1 (MQ=255)                                                                                                                                     ccGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGt                                                                                               >  1:47547/1‑251 (MQ=255)                                                                                                                                     ccGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGACGCGCATCGGGTTGATGGTA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTGAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATaaaaggtcgg                                                                                               >  2:38786/1‑244 (MQ=255)                                                                                                                                     ccGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATaaa                                                                                                      <  1:38786/244‑1 (MQ=255)                                                                                                                                       gcgTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTg                                                                                             <  1:283469/251‑1 (MQ=255)                                                                                                                                          tAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTc                                                                                          >  1:313148/1‑251 (MQ=255)                                                                                                                                              gttcatcaGAAGTGATGTCATCTTTCCGTACCATTGGCAGTTTGAAAATACGGAAGCTGATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGGATCGGTTTGATGATA‑CTCAAAGCCACAGCTTCTGGCTCAAGTC‑TACCGATGCGTTTCGGGGGTAAAGCCGTAAACATGATGAACATGCCAGTGACTAACGTGAAAGAGAGAATAAAGCTCAGTTGCTCagag                                                                                      <  2:227282/249‑1 (MQ=255)                                                                                                                                                 catcaGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCagagag                                                                                    <  1:214590/250‑1 (MQ=255)                                                                                                                                                          tGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGAc                                                                          >  1:303050/1‑251 (MQ=255)                                                                                                                                                            aTGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGGAGAACCACACCAACGGGGTGCAAACGTACAGGCCGAAGCGCATCGGGGTGATGATA‑CTCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGAGGCTTTTCGGGGTTAAATCCGCAAAAACGAGCAACATGCCAGTCACTAGCGCGAAGGAGAAAATAAAGCTCAGTTGCTCCAATAGCTGTGCCGACat                                                                        >  1:87244/1‑251 (MQ=255)                                                                                                                                                                           aCCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACa                                                          <  1:42506/250‑1 (MQ=255)                                                                                                                                                                            ccATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAgg                                                        <  1:26666/251‑1 (MQ=255)                                                                                                                                                                                tGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATTCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCtg                                                     <  1:182793/250‑1 (MQ=255)                                                                                                                                                                                 ggCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCtgt                                                    <  1:122475/250‑1 (MQ=255)                                                                                                                                                                                         gAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTg                                           <  1:365634/251‑1 (MQ=255)                                                                                                                                                                                                                         tGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGATAATGCCGCATCACCGACGCAAccgccg           >  2:259316/1‑251 (MQ=255)                                                                                                                                                                                                                                ccACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCg    >  1:216995/1‑251 (MQ=255)                                                                                                                                                                                                                                  acacCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAACGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAACCAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGCATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATCGCGGGAGAACAGGCTGTGAAAAGCTGTAGAACATGCCGCATCACCGACGATACCGCAGAGAATAGcc  >  2:187913/1‑251 (MQ=255)                                                                                                                                                                                                                                  acacCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCGcc  >  1:90426/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATA‑CGCAAAGCCACAGCTTCTGGCGCAA‑TCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCGCC  >  NC_000913/4440537‑4441012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑