Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,890	C→T	Q62* (CAG→TAG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,890	0	C	T	100.0%	18.9 / NA	8	Q62* (CAG→TAG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

AGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGA  >  NC_000913/4437655‑4438101                                                                                                                                                                                                                                            |                                                                                                                                                                                                                    aGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGGTGTCATATGTTACCCATGATCAGACACAATAATTTTAATGGTTCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGt                                                                                                                                                                                                       <  2:10562/250‑1 (MQ=255)                           ttAACCAAATAAAATAAGGTTGTCATATGTTAACCAGGATCAGACACAATAATTTTATTGGTGCGGAGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCATGTTAAATTTGAGGGAGTTCTTTCGTTGTGCAACTTAGATTGATAAAAGAGGTTATGAGGTGCTTTCATCAAAAAAt                                                                                                                                                                            >  2:256526/2‑251 (MQ=255)                           gTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAAt                                                                                                                                                                            <  1:70097/251‑1 (MQ=255)                                                                                                      ttGTAAAGTCTTCGTATACAAAAACACATTCAAACAATGTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGAc                                                                                                  <  1:270357/250‑1 (MQ=255)                                                                                                                              cacaTTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATAc                                                                         >  1:156906/1‑251 (MQ=255)                                                                                                                                                                                            aaTTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACAtt                                                                                               >  1:262891/1‑167 (MQ=255)                                                                                                                                                                                            aaTTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACAtt                                                                                               <  2:262891/167‑1 (MQ=255)                                                                                                                                                                                                      gCCTGTTAACTTTGAGGGCGGTCTTTCGTTGCGCCACTTAGATTGATAAAAGTGGTTATCAAGTGCTTTCATCAAAAAATGAAACTGTTCACGCAATGGATAAGTTTCTTCTCAGTTTCAGACTTAAAGATAACGGTGCCGAATATACCATGACATTGCGTGGTAGGGGATTTGAATAAGCAAAAATACCCATCACAATAAATGAATAACAGTCATTCATGGTCTTCAAAAACAGTGAATTTCCATTAGa  >  2:260547/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                    AGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGA  >  NC_000913/4437655‑4438101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑