Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,328,622	T→C	D496D (GAT→GAC)	rdcA →	regulator of diguanylate cyclase RdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,328,622	0	T	C	94.1%	91.5 / ‑3.3	36	D496D (GAT→GAC)	rdcA	regulator of diguanylate cyclase RdcA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (13/19);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGTTGTATGCTCTGCGATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTT  >  NC_000913/4328378‑4328862                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  cgctGTATGCTCTGCGATCCGCCTCGCTTAAACTTGTGAATTACGCGCAGCAGGCGCGGGAATACCGGGATTTTCTTGCGCACGGGTTAAACGTCGCTTTTGAACAATTGCGGCAAAATATACACCAGATCGAAGAAAGTTTGCAGCTATTGCAACGCAAGCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCGCCGTCATCCACTTTCTGCGTTAATAGCAAGACgcgc                                                                                                                                                                                                                                               <  2:375354/246‑1 (MQ=255)        tGCTCTGAGATCTGCCGCGAATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGAAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAAAAGCAAGACGCGCTGAAggggc                                                                                                                                                                                                                                      >  2:134380/1‑248 (MQ=255)            ctGCGATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAg                                                                                                                                                                                                                                    <  1:384602/249‑1 (MQ=255)             tGCGATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGtt                                                                                                                                                                                                                                  >  1:106693/1‑250 (MQ=255)              gtgATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGt                                                                                                                                                                                                                                   <  1:279482/246‑1 (MQ=255)              gCGATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCTGCAAAATATCCACCAGATCGAAGAAATTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCGGCGTCAACAGCAAGACGCGCTGAAGGTGCAGt                                                                                                                                                                                                                                   <  2:251487/248‑1 (MQ=255)               cGATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGCCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAgc                                                                                                                                                                                                                               >  2:144020/1‑251 (MQ=255)                                         ttACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGTTTAAACGTCGCTTGTGCACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTACAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGAGCGGAAGGTGCAGTTAGCCGCCTTGTTTTAGGATGCTTGGGAGc                                                                                                                                                                                                     >  2:142204/1‑251 (MQ=255)                                           aCGCTCAGCAGGCGCGGAAATACCTGGATTTTCGGGCGCCCGGGTTAAACGTCGCTTGTGAACATTTGCGGAAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCTAGATTAAACATGAAAGCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCg                                                                                                                                                                                                   <  2:382656/251‑1 (MQ=255)                                            cgcgCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGt                                                                                                                                                                                                  <  1:208562/251‑1 (MQ=255)                                                    agGCGCGGGACTACCTGGATTTTCGGGCGCGCGGGTTAAAAGCCGCTTGTGAACAGTTGCGGCAAAATTTCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACAGGAAATCGAGCTGGCCCTGCCCTCCGCCAACCACTTTCTGCGTCAACAGCAAGCCGCGCTGAAGGTGCATTTATCCGCCTTGTTTCTGGATTGTTCGGACCCGTTCAGCggg                                                                                                                                                                                          >  2:141404/1‑249 (MQ=255)                                                       cgcgGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGAtt                                                                                                                                                                                       <  1:258003/251‑1 (MQ=255)                                                            gAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTAc                                                                                                                                                                                  >  1:335336/1‑251 (MQ=255)                                                                               cgcACGGGTTAAACGTCGCTTGTTAACAAGTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCTAGAGTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCTACAGGCGTACCCGCTGTGAGACACTGTTa                                                                                                                                                               <  1:273032/251‑1 (MQ=255)                                                                                     ggTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACg                                                                                                                                                         <  1:38195/251‑1 (MQ=255)                                                                                                                ggCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGTCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTGCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACttt                                                                                                                              <  1:21785/251‑1 (MQ=255)                                                                                                                             ccaGATCGAAGAAAGTTTGCAGCTAGTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTg                                                                                                                <  1:176224/251‑1 (MQ=255)                                                                                                                                 aTCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCg                                                                                                             <  2:360750/250‑1 (MQ=255)                                                                                                                                          aGTTTGCAGCTCTTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGCAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGCGCAGTTAGCCGCCTTGTTTCAGGCTGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGACGAACACCATCAGCCGCGCCTTTACGC‑CGCGTTTTGCCGACCTTGAATc                                                                                                   >  2:54398/1‑251 (MQ=255)                                                                                                                                                     aTTGCAACTCAATCAGGCGCAGGTGGGTGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTg                                                                                          <  2:376582/249‑1 (MQ=255)                                                                                                                                                        gCAACTCAATTATGCGCCGGTGAGCGTCGAGATTAAACATGAACTCTAGCTGGCCCGTACCTCCGCCAACCACTTTTTGCGTCAACAGCAAGACGCGTTGTAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTGCCCGCTGGGAGACACTGTTACAGACGGCGCAGAACACCATCATTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAAGCCACCCTTTGCCggt                                                                                     <  2:370451/251‑2 (MQ=255)                                                                                                                                                              cAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTa                                                                                >  1:372858/1‑250 (MQ=255)                                                                                                                                                                  cAGGCGCCGGTGAGCGGCGAGATTAAACATGAAAACGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                                           <  2:199748/251‑1 (MQ=255)                                                                                                                                                                  cAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCg                                                                            <  1:193001/250‑1 (MQ=255)                                                                                                                                                                    ggCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGTAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATg                                                                         <  1:187934/251‑1 (MQ=255)                                                                                                                                                                                  gcgAGATTAAACATGAAAGCGAGTTGGCCCTGGCTGCCGCCAGCGCCGTTCTGCGTCAACAGGAAGCAGCGCTGAAGGTGCAGTTAGCCGTCTTGTTTCAGGATGATTCGGAGCCGTTAAGCTATATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAtt                                                           <  2:90732/251‑1 (MQ=255)                                                                                                                                                                                     agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg                                                        <  1:72972/251‑1 (MQ=255)                                                                                                                                                                                      gaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGc                                                       >  1:34111/1‑251 (MQ=255)                                                                                                                                                                                              aTGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTc                                               <  1:54398/251‑1 (MQ=255)                                                                                                                                                                                                          tGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                                   >  1:286853/1‑251 (MQ=255)                                                                                                                                                                                                                          cAACCACTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGATTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGttt                    >  1:356009/1‑250 (MQ=255)                                                                                                                                                                                                                               ccTTTCTGCGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCt              <  1:151515/250‑1 (MQ=255)                                                                                                                                                                                                                                     tGCGACAACAGCAAGACGCTCTGAAGGGGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGCGATTCGTACCCGCTGAGAGACACTTTTACAGACGGCGCAGAACAACATCAATAGCGACTTTACGCATG‑GGTTTGCCGAGCTTGAATCCACCCTTAGCCGAGTGTTAACCGATGTTATTCGCCCCCTTGAGCAACAAGTCAAAATGAAATTAAGCGAgaaagactgtcggcctgggttt        >  2:179337/1‑230 (MQ=255)                                                                                                                                                                                                                                       cGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTCCCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTTAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCACCAAGTCAAAATGGAATTGAGCGAGTCAGAGATTCGTCCTGGGttta       >  2:110301/1‑249 (MQ=255)                                                                                                                                                                                                                                       cGTCAACAGCAAGACGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGCGCACCAAGTCAACATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTc       >  2:73786/1‑250 (MQ=255)                                                                                                                                                                                                                                           aaCAGCAAGAAGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCAGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACAGCTGTTATTCGCCCCATTAAGCAACAAGTCAACATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCAtttt  >  2:367901/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CGTTGTATGCTCTGCGATCTGCCGCGCATAAACTGTTGAATTACGCGCAGCAGGCGCGGGAATACCTGGATTTTCGTGCGCACGGGTTAAACGTCGCTTGTGAACAATTGCGGCAAAATATCCACCAGATCGAAGAAAGTTTGCAGCTATTGCAACTCAATCAGGCGCAGGTGAGCGGCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGC‑TGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTT  >  NC_000913/4328378‑4328862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑