Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,321,488 G→A A70V (GCC→GTC)  phnG ← carbon‑phosphorus lyase core complex subunit PhnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,321,4880GA92.3% 140.0 / 0.2 55A70V (GCC→GTC) phnGcarbon‑phosphorus lyase core complex subunit PhnG
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (18/30);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

GGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCA  >  NC_000913/4321242‑4321718
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      
ggTCATGCGTTGTCTCCGCGAACCCTCGTAAAGAAGTCGACCCGTCTTGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTTCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGAcgcg                                                                                                                                                                                                                                    <  1:208687/251‑1 (MQ=255)
ggTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGAcgcg                                                                                                                                                                                                                                    >  1:193696/1‑251 (MQ=255)
          tGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCACTAAGGGTTTCTGATAAGTTTTGCAAGTGGCGAGATTGATGCATCAGCGCGTCAATCACCGCGCAGCGTTCGGCGTGCTGTTTATAACGCCCCTGCACCCAGCGGTAGCCGAGCGAGCCGTCAGTCCGGGGCACGGCGACGCGGGTGAGCGTg                                                                                                                                                                                                                          >  2:189534/1‑251 (MQ=255)
           gactcCGCGAACCATCGTAAAGCAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGACATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCACTCAGCGCGCAGCGTTCGGCGTGCTGTTTATCAAGCCCCTGCACCCAGCTGTAGCCGCGCGAGCCGTAAGTCAGGGGCACGGCGCCGCGGGTCAGCgagg                                                                                                                                                                                                                         >  2:240468/3‑248 (MQ=255)
                                       aCCCGGCTGGCGTTCCCTTCGGCCGGGCGTGCGGCAATGCGGGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGGCAATCAGCGCGCAGCGTTCTGCGTTCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGGGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGt                                                                                                                                                                                             <  2:50699/251‑1 (MQ=255)
                                       aCCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGt                                                                                                                                                                                             <  1:271672/251‑1 (MQ=255)
                                           ggCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTgccg                                                                                                                                                                                         <  1:224037/251‑1 (MQ=255)
                                                       cTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCcgcccc                                                                                                                                                                             >  1:17261/1‑248 (MQ=255)
                                                           ggctcggCGTGCGGCAATGCGTGCCATCCGTTCAGTGTCCAGGTGGGTAATACGGGGTGCTGTGAAGTTTTGAAAGTGGCGAGATTGCTGAAGCAGCGCGTCAATCTGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTATCCGAGCGGGCCGTCAGTCAGGCGCACGGTGACGCGGGTCAGCGTTGCGTAGCCGGCAAAAAACCGTTCGCCGGTGCCGCCCATCCGCGACTGaa                                                                                                                                                                         <  2:292393/246‑1 (MQ=255)
                                                              cTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCt                                                                                                                                                                      >  1:27288/1‑251 (MQ=255)
                                                                   gTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTT‑ATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCTGGTGCCGCCCATCCGCGCCTGAATCTGTACCa                                                                                                                                                                <  1:117994/251‑1 (MQ=255)
                                                                    ggCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCa                                                                                                                                                                <  1:52005/250‑1 (MQ=255)
                                                                     gCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAg                                                                                                                                                               <  1:353736/251‑1 (MQ=255)
                                                                             gCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGttt                                                                                                                                                       <  1:189534/251‑1 (MQ=255)
                                                                              cGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGACGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTc                                                                                                                                                      <  1:262711/251‑1 (MQ=255)
                                                                                tGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTc                                                                                                                                                      >  1:359791/1‑249 (MQ=255)
                                                                                         gTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGa                                                                                                                                           <  1:311433/251‑1 (MQ=255)
                                                                                            aGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGCCGCGGGTCAGCGTGGCGTCGCCGGCAAAAACACGATCGCCGGTGCCGCCGATCCACGCCTGAATCTGTACCAGGCCCGTTTCCGCGGCACGGATcc                                                                                                                                        >  2:345921/1‑250 (MQ=255)
                                                                                            aGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCa                                                                                                                                        <  1:355690/251‑1 (MQ=255)
                                                                                                              aaGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTTCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGa                                                                                                                      <  1:345846/251‑1 (MQ=255)
                                                                                                               aGGGTTGCTGATGAGTTTGGAAAGTGTCGAGATTGCGGCAGCAGCGCGGCAATCAGCGCGCAGCGTTGGGCGTGTCGTTTCTCACGCCCCTGCCCCCGGCTGTAGCCGAACGTGCCGTCAGTCAGGCGCACGGCGACGCGGGGCAGCGTTGCGGCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCGTCCGCGCCTGAAGCTGTACCAGGCCAGTTTCAGCGGCGCGTATCACCTCATAGTCGGCGTTGac                                                                                                                     <  2:130958/251‑2 (MQ=255)
                                                                                                                        gATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAgcgc                                                                                                            <  1:341685/251‑1 (MQ=255)
                                                                                                                        gATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAgc                                                                                                              <  1:235205/249‑1 (MQ=255)
                                                                                                                         aTAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGcgcg                                                                                                           >  1:304259/1‑251 (MQ=255)
                                                                                                                            aggtttGAAAGTGGCGAGATTGCTGCAGCCGCGCTTCAATCAGCGCGCGGCGTGCGGCGTGCTGTTTATCCCGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGGCAGGCGCACGGCGACGCGGTTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTTCCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTc                                                                                                        <  2:135961/248‑1 (MQ=255)
                                                                                                                                     aGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGtgct                                                                                               <  1:70225/251‑1 (MQ=255)
                                                                                                                                          cGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGACTCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGcc                                                                                            >  1:28107/1‑249 (MQ=255)
                                                                                                                                             gaTTGCTGCATCCGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGGGCCGTCAGTCAGGCGCACGGCGACGCTGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTc                                                                                       <  2:187360/251‑1 (MQ=255)
                                                                                                                                                    gcATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAACCGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTCGCGCGTTCAGGCGTGCTGCCAGTTCAGACGGt                                                                                >  2:309429/1‑251 (MQ=255)
                                                                                                                                                    gcATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGt                                                                                <  1:205011/251‑1 (MQ=255)
                                                                                                                                                     cATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGtt                                                                               <  1:240468/251‑1 (MQ=255)
                                                                                                                                                                  aTCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAg                                                                  <  1:189371/251‑1 (MQ=255)
                                                                                                                                                                         cgcAGCGGTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGAc                                                           <  1:282644/251‑1 (MQ=255)
                                                                                                                                                                            aGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATc                                                        <  1:167252/251‑1 (MQ=255)
                                                                                                                                                                              cGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATcc                                                                                                                                                                                  >  1:22633/1‑127 (MQ=255)
                                                                                                                                                                              cGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATcc                                                                                                                                                                                  <  2:22633/127‑1 (MQ=255)
                                                                                                                                                                              cGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATcc                                                       >  1:265964/1‑250 (MQ=255)
                                                                                                                                                                              cGTTAGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGGCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAACAAAACGGTCGCCGGTGCCGCCCCTCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGCGCACATCATAGGCGGCGGTGATGTTTAGAGCGGTCCGGGCAGCAGCCAGGTCAGCCGGTTGGcgaggcgacagcgcgggaatcc                                                       >  2:10006/1‑229 (MQ=255)
                                                                                                                                                                                ttCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGt                                                    >  1:206425/1‑251 (MQ=255)
                                                                                                                                                                                 tCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGt                                                    <  1:201375/250‑1 (MQ=255)
                                                                                                                                                                                   ggCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACAGACATCCAGTGCt                                                 >  1:79085/1‑251 (MQ=255)
                                                                                                                                                                                     cGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGACGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGGTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGGTTCAGCGGCGCGGATCACCTCATCGTCGGCGGTGACGTTTTGCGCGTTCAGGCGGGCTGCCAGTTCAGCCGGTTGGCTGTGCGcccccccggacaccccgcgccgc                                               >  2:4197/1‑230 (MQ=255)
                                                                                                                                                                                               aTCACGCCCCTGCACACAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGGGACGCGGGACAACATGGCGTCGCCGGCAAAAAAACGTTCGCAGGTGCAGCCCATCAGCGCCTGAATCTATACCAGGCCAGTTTCAGCGGCGCGGAACACCTCAAAGTCGGCGGTGACGTATAGCGCGTTCAGGGGGGCCGCCAGTTCAGCCGGTTGGGTGTGGGCCAGCGCGTACATCCAGtagttgagggtggcgg                                     >  1:294579/1‑236 (MQ=255)
                                                                                                                                                                                                 cACGCCCCTGCTCCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCGCGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTa                                   <  1:353273/251‑1 (MQ=255)
                                                                                                                                                                                                 cACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGAGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCgggg                                   >  1:342757/1‑249 (MQ=255)
                                                                                                                                                                                                  aCGCCCCTGCACCCAGCTGTATCCGCGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGTCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCATGCCAGTTTCAGCGGCGCGGATCCCCTCATCGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCCCGGACCTCCAGTGCTGGCTGGTCTCgggat                                  >  1:112261/1‑248 (MQ=255)
                                                                                                                                                                                                    gCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGAACGGACATCCAGTGCTGGCGGGGCGCgggcttt                                >  1:336898/1‑246 (MQ=255)
                                                                                                                                                                                                             cccAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCAtt                       <  1:86244/251‑1 (MQ=255)
                                                                                                                                                                                                              ccAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATTACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTc                      <  1:289899/251‑1 (MQ=255)
                                                                                                                                                                                                                       gctgggCGTGCCGTCAGTAAGGCGCATGGCGATGCGGGTCGGCGTGGCGTCACGGGCAGAAAAACGTTCGCCGGTGTCTCCCTTCCTCTCCTGAAGCTGTACCAGGCCAGTTTCAGCGGCGCCTATCACCTCATAGTCGGGTGTGACGTTTAGCGAGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCGGTGCGCCAGCACGGTCATCCAGTGCTGTCGTGTCTCGGTATCGGCTTGCATTCatttcgcc              <  2:352853/245‑8 (MQ=255)
                                                                                                                                                                                                                              gTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGaa      <  1:329880/251‑1 (MQ=255)
                                                                                                                                                                                                                               tGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGGATCTGCgggccataagggcttcctagggaat     >  1:176961/1‑227 (MQ=255)
                                                                                                                                                                                                                                gCCGTCAGTCAGGCGCACGGCGCCGGGGGTCAGCTTGGCGTCGCCGGCAAAAAAGCGTTCGCCGGTGCCGCCCATCCGGGCCTGAATCGGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGGTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGCGTGCATTCAGTGCGCCATAGTGAAtt    <  2:182258/251‑1 (MQ=255)
                                                                                                                                                                                                                                 ccGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTc   <  1:7727/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gtcagtcaGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGGGGGCATTCAGTGCTCCATAGTGGAttta  >  1:87729/1‑248 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      
GGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCA  >  NC_000913/4321242‑4321718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑