Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,320,416 G→A T83I (ACC→ATC)  phnI ← carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,320,4160GA96.2% 144.4 / ‑2.6 53T83I (ACC→ATC) phnIcarbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (28/22);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

CCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGG  >  NC_000913/4320176‑4320653
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
cccTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTTGgc                                                                                                                                                                                                                                     >  1:53614/1‑251 (MQ=255)
cccTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCAGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGGAGgc                                                                                                                                                                                                                                     >  1:253791/1‑251 (MQ=255)
       gcgcCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCgcagc                                                                                                                                                                                                                              <  1:230951/251‑1 (MQ=255)
          ccAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAgg                                                                                                                                                                                                                           <  1:120644/251‑1 (MQ=255)
               agGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGGAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCCGCTTCGCCAACGTGATGCGGTAGGGGCGCAGCAGGaaaa                                                                                                                                                                                                                       >  1:198933/1‑250 (MQ=255)
                     aaaaCGTGCGGGGTAGGCGGCTGGTCGCTGCAGGCGGTGGTCAGCGTGGGCGCTTCGGTGATGGCCAGCAGGGAAAAAGCGAGCAGGCGAGTGGTATAGTCGCAGGTTGGGCCAGGTAGCTGGCTGCGGGGAATGTTTTTATAAACGACGGAGAGCCGGCGTCTGATGCGGATCCCGGTGGTGTCGAGTGTCTCGCTTATCGCCAGCTTCTCCAACGTGAGGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                                                                                                                                                                                <  2:37163/251‑1 (MQ=255)
                     aaaaCGGGCGGCGACGGCTGCTGTTCGCTGTCGGCGTTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGTCGATGGGTGTAGTCTTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                                                                                                                                                                                <  1:27963/251‑1 (MQ=255)
                                   cggatgcttctCGCTGTCGGTGGGGGTCAGCGGCGGCTCTTGGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGGCGTAGGTTGGGCCATGTAGCTTGCCGCCGGGAATGTTGTTATAAACGACTGAGATACGGCGTTCGGGTCGCATCCCGGTGTGGTCGAGCGGCTCGCTTCCCGCCAGCTTCGCCAACTTGATGCGGTAGGCGCGCAGCAGGTAAATCGCTTCAACGTTATcgccgc                                                                                                                                                                                                  <  2:222664/241‑1 (MQ=255)
                                        gctgTTCGCTGTCGGCGGTGGGCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTGATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGcc                                                                                                                                                                                             <  1:243540/251‑1 (MQ=255)
                                        ctgattccagtgcggcgtgggtCAGTGTGGGCGCTGCACCGTGTGCCAGAAGGGTAAACGCGAGCGGGCGATGGGGGTAGTCGTAGGTTGGGCCAATCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGTGGATACGGCGTTCGAGACGCCTCCCGGTGGTGTGGAGCGGCTCGTTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTgg                                                                                                                                                                                               <  2:359217/230‑1 (MQ=255)
                                                   tCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgc                                                                                                                                                                                  >  1:380585/1‑251 (MQ=255)
                                                         gtggtCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                                                                                                                                                                            >  1:357064/1‑251 (MQ=255)
                                                         gtggtCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                                                                                                                                                                            >  1:247783/1‑251 (MQ=255)
                                                           ggtCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGGAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCAAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGcgccg                                                                                                                                                                          <  1:79006/251‑1 (MQ=255)
                                                             gcagcttcgggccTTCGCCGTTGTCCAGCGGGGTAAATTCGATCAGGCGATGGTTGTAGGCGTAGTTTTGGCCAAGCAGCGGTACTCCGGGAAGTTCTTTATAACCGGCGGCTATACGGCGTCCGAGACGCATCCCGGTGGGGTCGAGCGGCCCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGGAGGCGCTCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCgccgcc                                                                                                                                                                        <  2:328711/239‑1 (MQ=255)
                                                               aGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCgccgcc                                                                                                                                                                        >  1:6058/1‑249 (MQ=255)
                                                                          ttCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCgg                                                                                                                                                           >  1:371851/1‑251 (MQ=255)
                                                                           tCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGc                                                                                                                                                          >  1:211805/1‑251 (MQ=255)
                                                                                     ccAGCAGGGTAAAATCGAGCGGGCGTGGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGGGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCt                                                                                                                                                <  1:142641/251‑1 (MQ=255)
                                                                                          agGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGt                                                                                                                                           <  1:18010/251‑1 (MQ=255)
                                                                                             gTAAAATCGCGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCAGCCGGGAATGTCTTTATAAAAGACGGAGATACGGCGATCGAGACGCATACCGGTGGTGTAGAGCGGCTCGCTTACCGCCCGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCCGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGGTCGCGGTCGGCAATGCCGCCTTCGGtcat                                                                                                                                        >  1:222554/1‑251 (MQ=255)
                                                                                              tAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGCCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGAAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTGCGCGGTCGGCCATGCCGCCTTCGGtcatc                                                                                                                                       >  1:34376/1‑251 (MQ=255)
                                                                                                    cGAGCACGCGCTGGGTGTAGTCGTAGGCTGGGCCAAGCAGCTGGCCGACGGGAATGTCTTTATAAACGCCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGCGCGGCTCCCTTACCACCAGCTTCGCCAACGTGATGCGGTACGCGCGCAGCAGGAAAATCGCTTCAACGTTATTCCCGCCGGCCTGTTTCAGCGCCAGCCCCGGCAGGTCGCCGTCGGCAATGCCGCCTTCGGTCCTCAcccgg                                                                                                                                 >  2:236534/1‑247 (MQ=255)
                                                                                                      aGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCACCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATc                                                                                                                               <  1:383805/251‑1 (MQ=255)
                                                                                                            ggATGGGTGTAGTCGTAGGGTGGTCCAAGCAGTGGGCCGCCGGGAATGTTTTTATAAACGACGGGGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACcgc                                                                                                                         <  1:166266/250‑1 (MQ=255)
                                                                                                             gATGGGAGTAGTCGTAAGGTGGGCCAAGCAGATGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCACCGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAACGACGCTGGCCTGTTTCCGCGCCCGCGCGACCAGTTCTCGGTCGGCAATGCCCCATTCGGGCATCACGCGGTCTACcgcg                                                                                                                        >  2:92425/1‑251 (MQ=255)
                                                                                                                  gtgtAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAACCGACGGAGCTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTCCCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCCCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGtt                                                                                                                   >  1:187815/1‑251 (MQ=255)
                                                                                                                    gtAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTa                                                                                                                  >  1:361676/1‑250 (MQ=255)
                                                                                                                            aGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAActgctg                                                                                                          <  1:39385/250‑1 (MQ=255)
                                                                                                                                         cagcTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGCGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCg                                                                                             >  1:184130/1‑250 (MQ=255)
                                                                                                                                         cagcTGGCCGCCGGGAATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCgg                                                                                            >  1:166175/1‑250 (MQ=255)
                                                                                                                                            cTGGACGCAGGGAATGTCTTTATAAACGACGGCGATACGGCGTTCAAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCACCGTGATGCGGGAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGATTCAGCACCAGCGCCGCCCGTTAGATGGCGGCAATGCCTACTTCGGTCATCCGGCGATCTACCGCGAGGTTAAACAGCTGGTCAATCTGGGGGACGc                                                                                         >  2:200910/1‑251 (MQ=255)
                                                                                                                                                 cgccgGGGATGTCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTTCTGTTCAATCTGCGCGACGCTCAGt                                                                                    >  1:347503/1‑251 (MQ=255)
                                                                                                                                                        atgggcTTTATAAACGCCGGAGATTCGGCGTTCGGGCCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCa                                                                             <  1:199623/246‑1 (MQ=255)
                                                                                                                                                            tCTTTATAAACGACGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATc                                                                         >  1:341021/1‑251 (MQ=255)
                                                                                                                                                                              aTACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCg                                                       >  1:52666/1‑251 (MQ=255)
                                                                                                                                                                                    cGTTCGAGCCGCATCCCGGTGGTGTCGAGCGGCCCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGctctc                                                 <  1:4950/251‑1 (MQ=255)
                                                                                                                                                                                        cGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTAAATCTGGGCGACGCTCAGTTCGGGCAAATCGGAATCGCCTCGGCGTCGGCTCTCTTGc                                             >  1:155186/1‑251 (MQ=255)
                                                                                                                                                                                             cGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCCAATCGGTATCGCCTCGGCGTc                                                        >  1:31604/1‑235 (MQ=255)
                                                                                                                                                                                              gCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCg                                       <  1:228159/251‑1 (MQ=255)
                                                                                                                                                                                               cATCCCGGTGTTGTCAAGCGCCTTCCTTCCCGCCAGCTTCGCCACCGTGAGGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGt                                      <  1:140372/251‑1 (MQ=255)
                                                                                                                                                                                                  ccggggggggtggaggggcctgctcaCCGCCAGGTCCGCCAACTTGCTGCGGTAGGCGCGAGGTGGGAAAACCGCTTCAGTGGTATGGCCGCTGGCCTGTTTCGGCGCCGGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCAGCCCTCGATCTACCGCGAGGTTAAACTGCTGTTCAAGCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTggg                                   <  2:143917/226‑1 (MQ=255)
                                                                                                                                                                                                    cGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTggg                                   >  1:255174/1‑249 (MQ=255)
                                                                                                                                                                                                       gggggtggcgcgcaccGCTTACACCCAGCGTCGCCAGCGTGATGCGGTGGGCGCGCAGCAGGAAAATCGCTTAAACGTTACCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGCCGCTCAGTTCGGGCAAAGCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTTGgcggcg                              <  1:206502/236‑1 (MQ=255)
                                                                                                                                                                                                             gggtcGGCTCGCTCACCGCCAGTTTCGCCAACGTGAGGCGGTGGGCGCGAGGAAGGAAATTCCATGTATCGTAACCGCCGCTGGCCTGTTTCAGCGCCATCGCCGCCAGTTCGCGGTCGGCAATTCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGTTATCGCCTCGTCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATc                        <  2:371851/247‑1 (MQ=255)
                                                                                                                                                                                                             cGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCGGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGAt                         <  1:234500/250‑1 (MQ=255)
                                                                                                                                                                                                                      cGCTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCg               >  1:354313/1‑251 (MQ=255)
                                                                                                                                                                                                                        cTTACCGCCAGCTTCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGcc             >  1:123253/1‑251 (MQ=255)
                                                                                                                                                                                                                          tACCGCCAGCTTCTCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGccc            <  1:64164/250‑1 (MQ=255)
                                                                                                                                                                                                                          tACCGCCAGCTGCGCCAACGTGATGCGGTAGGCGCGCAGCAGGAAAATCGCTTCACCGTTATCGCCGCTGGCCGGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGccc            <  1:64156/250‑1 (MQ=255)
                                                                                                                                                                                                                            ccGCCAGCTTCGCCAACGTGATACGGTAGGCGAGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGGTAAACTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTCTCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCt         >  2:252229/1‑251 (MQ=255)
                                                                                                                                                                                                                               cccgattCGCCAACGTGATGCGGTAGACGAGCAGCCGGAAAATCGATTCAACGGTATAGCCGCTGGCCTGTTTCGGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACAGCGAGGTAAAACGGCTGTTAAATCTGGGCGACGATCAGTTCGGGAAAATCGGTATCGCCTCAGCGGCGGCTCTCTTGCAGGGCGTGGGCGGCGGCGATCGCCTTCTCGCCCCCtgtc      >  2:354971/6‑248 (MQ=255)
                                                                                                                                                                                                                                   cTTCGCCAACGAGATGCGGGAGGCGCACAGCAGGAAAATCGCTTCAACGATATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTAGGCACTGCCGCCTTCGGTCATCAAGCGATCTACCGCGAGGTAAAACTGCTGGTCAAGCTGGCCGACTCCAAGTTCGGGCAACTCGGAAACGCATCGGCGGCGGCTATCTTGCCGGGCGTGGGAGGCGGCGGTCGCATTCTCGCTCCCTTACACgg  >  2:304347/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
CCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCGGCAATGCCGCCTTCGGTCATCACGCGATCTACCGCGAGGTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGG  >  NC_000913/4320176‑4320653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑