Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,307,151 G→A P217S (CCT→TCT)  alsK ← D‑allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,1510GA88.9% 53.5 / ‑0.4 27P217S (CCT→TCT) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (11/13);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

GATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAG  >  NC_000913/4306908‑4307397
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
gATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGttt                                                                                                                                                                                                                                                 >  1:178846/1‑251 (MQ=255)
       cAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCAt                                                                                                                                                                                                                                          <  1:1257/251‑1 (MQ=255)
         atatTGCTGCGCCCTGAGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATAGCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATgg                                                                                                                                                                                                                                        <  1:23022/251‑1 (MQ=255)
           atttcttatctcTGAGCGCCATTAAAGTCAGAAGAGGTGGCATCAATAAAGCGCGCGCCCTGATGCGGCAGAGGGCGGCGCAGGCACTTTTGGGTCAGGGCAACGATAGTCGCGCGTGTGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGa                                                                                                                                                                                                                                       <  2:304367/239‑1 (MQ=255)
             tgctgcACACTGAGCGCCATTAAAGTCAGAAGGTGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCCGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCCTCGGGATCGAAAAGGTTAATGCTGGTGGCAAGGGCCAGTGCCGCGTTTTCAAACAGACTCTGGACGAAAGAGACGTTTTCCGCATGGACg                                                                                                                                                                                                                                     >  2:219250/1‑250 (MQ=255)
              gctgcACCCTGATCGCCATTAAAGGCAGAATATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCATGTACATTTGGGTCATGGCAACGAGAGTCTCTCGTGGGAAGGCGGGCATAGCCATCACGCCACCGCCCAGGATCCCCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGGGTTGTCAATCAGACTCTGGACGAAAGAGGCGTTTTCCTCATGGACGaa                                                                                                                                                                                                                                   <  1:333161/251‑1 (MQ=255)
                         aGCGCCATTAAAGTCAGAAGATGAGGCAGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGAACGAACAGATTAATGCTGGTGGCAATGGCCAGTGCAGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCacgc                                                                                                                                                                                                                         >  2:193770/1‑250 (MQ=255)
                                                         tAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTAcc                                                                                                                                                                                          >  1:106521/1‑249 (MQ=255)
                                                                      cTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGcc                                                                                                                                                                            >  1:317219/1‑250 (MQ=255)
                                                                                    gACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTg                                                                                                                                                             <  2:106521/251‑1 (MQ=255)
                                                                                            aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAg                                                                                                                                                     <  1:134729/251‑1 (MQ=255)
                                                                                               tACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGcc                                                                                                                                                  >  1:286636/1‑250 (MQ=255)
                                                                                                atttttGGGTCATGGCAACTAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATAGAACAGATTAATTCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGTCGAAAAGATCGCGCAATGGGTAATTGCGGGGCTTTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCa                                                                                                                                                  <  1:232629/248‑1 (MQ=255)
                                                                                                          cATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTg                                                                                                                                        <  1:193770/250‑1 (MQ=255)
                                                                                                             ggCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCca                                                                                                                                     <  1:219250/250‑1 (MQ=255)
                                                                                                              gCAACGAGAGGCGCGCGTGGGAAGGCGGGCATATCCATCACGCCACCTCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGATTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATTGGTAATTTCGGGGCTGTTGTTCGAACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCcaca                                                                                                                                   <  2:317219/251‑1 (MQ=255)
                                                                                                                               ggggAAGGGGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCGGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGTGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCa                                                                                                                  <  2:286636/250‑1 (MQ=255)
                                                                                                                                       cGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTccc                                                                                                          >  1:338651/1‑251 (MQ=255)
                                                                                                                                           cATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggg                                                                                                      <  1:300101/251‑1 (MQ=255)
                                                                                                                                                                                ggATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCTATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGATGGGTCATATCTCCCa                                                                                                         <  2:207119/211‑1 (MQ=255)
                                                                                                                                                                                ggATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa                                                                                                         >  1:207119/1‑211 (MQ=255)
                                                                                                                                                                                                          aaTGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAAGCGTTTTCCGCCTGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAAGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATTTCCCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCACCGGCGCAcactttatccccc                                       >  2:122708/1‑239 (MQ=255)
                                                                                                                                                                                                            gggCCCGTTCCGGTTTTCCAACCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGAGCGCGCAATGGGTAATTCCGGGGCTGTTGTTCGTACCAGCGTTTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCGCGCAGTGTTGGGTCATATCCCCCGGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCacac                                      <  1:211253/249‑1 (MQ=255)
                                                                                                                                                                                                             ggCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGAGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGACTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTg                                    >  1:290521/1‑251 (MQ=255)
                                                                                                                                                                                                                             tCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGgcg                                                      >  1:355390/1‑217 (MQ=255)
                                                                                                                                                                                                                                 attaGACTCTGGACGAAAGATGCGTTTTCCGCATGTACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGAGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCGATGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTACGCCTGCCACACCGTGTGCACCCGTCCAAGGAGCACCTGTCATCCCCGCTGCGCACACCATCCCCGTAcc                 >  2:134051/4‑251 (MQ=255)
                                                                                                                                                                                                                                                aaaGAGGCGTTTTCAGCATGGACGAAAAGATAGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGCTATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCCTCCACACTGCGAACCCCATCCCCGAACCGAGATAGgccgcccg  >  2:53374/1‑249 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
GATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAG  >  NC_000913/4306908‑4307397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: