Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,646	G→A	A86V (GCC→GTC)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,646	0	G	A	97.4%	113.5 / ‑4.4	39	A86V (GCC→GTC)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (20/17);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

GCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCA  >  NC_000913/4284399‑4284879                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          gcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGAAAACACCAGCGCGGAGATCCCCAAGAATGAGGcgacgg                                                                                                                                                                                                                                        >  1:139126/1‑251 (MQ=255)               ggATAAGGTAAAGCGCAGCCACCACCAGAGAAACACAGGCCGAAAGAATACGAAGCGGACCTTGTTTCAGACGGTAAGAGGCCACAGCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTACGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAATCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGATGCGACGGACATATAGGCCCCg                                                                                                                                                                                                                          <  2:373154/250‑1 (MQ=255)                      gTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCg                                                                                                                                                                                                                   <  1:6666/251‑1 (MQ=255)                         aaGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCACTCGcc                                                                                                                                                                                                                 >  1:383388/1‑250 (MQ=255)                                                    ggCCGAAAACATACGAATCGGCCTTTGTTTCAGACGGTAAGAGGCCACACCGGCAAATGTGTAGCTCCTCAGGTCTCGCATACGGTCGCCAATCTGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGGCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCTGCAATCGCCAGCCCGTTCTGGAAGTCAGTGATATTgc                                                                                                                                                                                     <  2:13126/251‑1 (MQ=255)                                                       cGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTgccgc                                                                                                                                                                                  >  1:61875/1‑251 (MQ=255)                                                           aGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGc                                                                                                                                                                              <  1:172319/251‑1 (MQ=255)                                                                      cgacccTTGTTTCAGACGGTAAGAGGCCAAATCGGAAACGGTGTAGCGCCCCATGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCACACCAGGAAGCCCAGCGAGTAAATTCAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATACATTCCCCGGCAATCGCCAGCCCGGTCTGGAATACAGTGAAATTGTCGCCAGCGGCgtatgagg                                                                                                                                                                   >  2:336221/4‑246 (MQ=255)                                                                      cGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTagtagt                                                                                                                                                                   <  1:50458/251‑1 (MQ=255)                                                                        gCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGa                                                                                                                                                                                                    >  1:73207/1‑216 (MQ=255)                                                                        gCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGa                                                                                                                                                                                                    <  2:73207/216‑1 (MQ=255)                                                                             tGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTAc                                                                                                                                                             <  1:313304/250‑1 (MQ=255)                                                                               tttCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACg                                                                                                                                                            >  1:319484/1‑249 (MQ=255)                                                                                  cAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGaa                                                                                                                                                        <  1:155173/250‑1 (MQ=255)                                                                                   aGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACg                                                                                                                                                      <  2:151537/251‑1 (MQ=255)                                                                                        gTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGATACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTAcgc                                                                                                                                                 >  1:122672/1‑251 (MQ=255)                                                                                           agagGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGtt                                                                                                                                              >  1:44141/1‑251 (MQ=255)                                                                                            gagGCCACAACGGCAAAGGGGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATGAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGttt                                                                                                                                             <  2:44141/251‑1 (MQ=255)                                                                                                    aTCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGccc                                                                                                                                     <  1:318747/251‑1 (MQ=255)                                                                                                      cGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGCTGcccgg                                                                                                                                   >  1:47362/1‑249 (MQ=255)                                                                                                            aGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCGATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGt                                                                                                                              >  1:106932/1‑250 (MQ=255)                                                                                                                              tACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACacg                                                                                                           >  1:263237/1‑251 (MQ=255)                                                                                                                               aCGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCAACAGGAAGCCCAGCGCGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGCGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGAGTAGTAGTCGCTACGAGAACGTACGCGGTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACacg                                                                                                           >  2:142345/1‑250 (MQ=255)                                                                                                                                 gCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACat                                                                                                                                                                                                                                    <  1:119755/127‑1 (MQ=255)                                                                                                                                 gCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACat                                                                                                                                                                                                                                    >  2:119755/1‑127 (MQ=255)                                                                                                                                          cGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAgg                                                                                               >  1:347778/1‑251 (MQ=255)                                                                                                                                              aaTCAGGAACAAAATGATCGGCCAGCCCACCAGGACGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACCCCAGCGCGGAGAGCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCGGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACa                                                                                           <  1:40102/251‑1 (MQ=255)                                                                                                                                               aTCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGTGCGTAAACACGACGAAAATCAGGAACat                                                                                          <  1:138117/251‑1 (MQ=255)                                                                                                                                                 cAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGCGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGGAAACACGACGAAAAACAGGAACata                                                                                         >  2:74110/1‑250 (MQ=255)                                                                                                                                                                  gCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCACGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGGGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTc                                                                       <  1:180891/251‑1 (MQ=255)                                                                                                                                                                     agtccaccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACTCCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGGGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAACCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGtt                                                                    <  1:304474/248‑1 (MQ=255)                                                                                                                                                                        ccaccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCAACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggc                                                                 >  1:245776/1‑251 (MQ=255)                                                                                                                                                                         caccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGTCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTaa                                                                                                                            <  2:329859/191‑1 (MQ=255)                                                                                                                                                                         caccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTaa                                                                                                                            >  1:329859/1‑191 (MQ=255)                                                                                                                                                                          accaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggctg                                                               >  1:299075/1‑251 (MQ=255)                                                                                                                                                                                        cGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGccc                                                 >  1:270984/1‑251 (MQ=255)                                                                                                                                                                                          aGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCGGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCg                                               <  2:299075/251‑1 (MQ=255)                                                                                                                                                                                                                    gTAAACCCCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGc                     <  1:54554/251‑1 (MQ=255)                                                                                                                                                                                                                      aaaCACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCg                    >  1:11765/1‑250 (MQ=255)                                                                                                                                                                                                                                         cccAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCa  >  1:105734/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          GCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCA  >  NC_000913/4284399‑4284879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑