Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,283,465 G→A P480S (CCG→TCG)  actP ← acetate/glycolate:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,283,4650GA90.0% 71.8 / ‑1.1 30P480S (CCG→TCG) actPacetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (15/12);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTT  >  NC_000913/4283225‑4283710
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
cgggcAGACGACCGGGGAAGGGGAGAGATTAAGGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGatc                                                                                                                                                                                                                                             <  2:17771/247‑1 (MQ=255)
  gTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGatcat                                                                                                                                                                                                                                           >  1:368438/1‑251 (MQ=255)
                               aTGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCccagcc                                                                                                                                                                                                              >  1:73102/1‑251 (MQ=255)
                                       ggCCTTGCTCAACGCCAAAGCCGGTCTGGGCGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCAAccc                                                                                                                                                                                                      >  1:310796/1‑251 (MQ=255)
                                             ccTCACCGCCTAATCCGGTCTGGGAGCGGATAA‑CTGGCGAGCGGAACAGTGAACGCGCCTCCGGGCCAGCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATATAGAAC‑AGCGCTGGGTAGTCATACGGGAAGATGGCTTTTTCGTGTCCAAGGATCTGTTCCCAAAGCGTCGATCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCcatcagc                                                                                                                                                                                                <  2:245376/250‑3 (MQ=255)
                                                  aCGCCAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGcc                                                                                                                                                                                           >  1:90359/1‑251 (MQ=255)
                                                      cAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCAcgc                                                                                                                                                                                       >  1:184921/1‑251 (MQ=255)
                                                         aGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTg                                                                                                                                                                                    <  1:274346/251‑1 (MQ=255)
                                                              gTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTc                                                                                                                                                                                 >  1:298175/1‑249 (MQ=255)
                                                                  gggAGCGGATAAACGG‑CGCGCGGAATAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGATGAACCAGATGCCGAGGAATGCCACGGTGATTGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTTACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCTCCCATCATCGCGCCACGCGTGGTCAGTTTc                                                                                                                                                                           <  2:263039/251‑1 (MQ=255)
                                                                            aaaCTG‑CGCGCGGAACAGTTCACGCTCACGCGCTCCTTACGCTGAGTGATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGACGGGTATTCATACGGGAAGATGGCTTTTTAGCGACCAAGGATCTGTACCCAATACGTCGAGCCGAGGATCCTCAGAACTCCTGCGGTAATACTCCCCAGCAAGCCACCCATCATCGAGCCaagagcggccagtttcaacaacgac                                                                                                                                                                  >  2:237773/1‑226 (MQ=255)
                                                                                      gcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAg                                                                                                                                                              <  1:119206/245‑1 (MQ=255)
                                                                                      gcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGGATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGCACATAgggatcg                                                                                                                                                        >  2:119206/1‑245 (MQ=255)
                                                                                                   cgctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGaa                                                                                                                                           <  1:81438/251‑1 (MQ=255)
                                                                                                                 ttCCGCTGAGTTATCGGATGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGAGGGCTTTTTCGTGACCACGGATCTGTACCCAAATCGTCGAACCGAGGATCATCAGTACGACTGCGGTTATCAGCCCCAGCCAGCCCACCATCATCGCGCCACGAGTGGACAGTTTAGACAAGTACATCGAAAGCAGAAGGCTCGGGAGGTTACAGCTcgccc                                                                                                                             >  2:991/1‑250 (MQ=255)
                                                                                                                                            gaaCCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCCTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATa                                                                                                   >  1:126812/1‑250 (MQ=255)
                                                                                                                                                 aGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTTCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCg                                                                                             >  1:23175/1‑251 (MQ=255)
                                                                                                                                                          ggAATGCCACGGTGAAAGAGAACGAG‑GCCGGGTAGTCATACGGGAAGATGGTTTGTTCGTGACCAAGGATCTGTCCCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAACCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGa                                                                                    <  2:184921/251‑1 (MQ=255)
                                                                                                                                                                                  aGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGAt                                                             >  1:77851/1‑251 (MQ=255)
                                                                                                                                                                                            aTTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGACGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCAACATAAAGGCGATGTTCTGATTCTCAAACACCACGCCGAGGATAATCGCAATc                                                   >  2:245208/1‑251 (MQ=255)
                                                                                                                                                                                                              cTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGCAAGCAGACTGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCCCCCTCAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGCATAATCGCAATCACGCCGAGGATCAGTACg                                 >  2:177487/1‑251 (MQ=255)
                                                                                                                                                                                                               tttttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACgg                                >  1:287874/1‑251 (MQ=255)
                                                                                                                                                                                                                ttttCGAGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCCGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGCTGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGt                               >  2:308427/1‑251 (MQ=255)
                                                                                                                                                                                                                 tttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGt                               <  1:245208/250‑1 (MQ=255)
                                                                                                                                                                                                                   ccggtaaccaagttctggccccAAACCGCCGAGCCGAGGATCAGCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCTAGGATCAGTACGGTGAt                            <  2:368438/232‑1 (MQ=255)
                                                                                                                                                                                                                     gTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGAttt                          <  1:237773/251‑1 (MQ=255)
                                                                                                                                                                                                                               aacggttcccAAAGCGCCGAGCCGTGGATCATCAATTCTTCGGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCg                <  2:298175/244‑1 (MQ=255)
                                                                                                                                                                                                                                   gTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGc            <  1:315471/251‑1 (MQ=255)
                                                                                                                                                                                                                                     aCCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGctc          >  1:148872/1‑251 (MQ=255)
                                                                                                                                                                                                                                             cgtcgGGCCGAGGAGCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGAGCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGtt  <  1:311712/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
CGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTG‑CGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAC‑AGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTT  >  NC_000913/4283225‑4283710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑