Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,093	G→A	A372T (GCG→ACG)	yjcE →	putative transporter YjcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,093	0	G	A	97.4%	102.3 / NA	39	A372T (GCG→ACG)	yjcE	putative transporter YjcE
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (15/23);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGGCGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATG  >  NC_000913/4280855‑4281338                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      gCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTg                                                                                                                                                                                                                                            <  1:265426/250‑1 (MQ=255)           ggTATTCTGGAGACGTCACTGCTGGCAGCAGAAATCGCCCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATCTGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGGGGACGAGGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGGAGCCGATGGCGGTTGGTTCGTGGACCACACGAGAAGTCCTGATCGCGTCTTTCGCAGGGGTGCGTGGGACGCTCACTCTGGCCGGAGTGct                                                                                                                                                                                                                                 >  2:48384/1‑251 (MQ=255)            gTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGctc                                                                                                                                                                                                                                <  1:307097/251‑1 (MQ=255)                     agaCGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCGGGATGCTGTGTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGGTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCg                                                                                                                                                                                                                       <  2:337562/251‑1 (MQ=255)                                              ggccccTAACGTCTAAAACTGGATGGTGTTTACCAAGATTATGTTGATATATGCGGCGCGTATGCTGGTCCGTTTCGGCTGGCTGGGGACGATGAAAAAGTTCAGCAACCTCTTCCTGAAGAAGAAGCCGAGGGAGTTTGGGTCGTGGACCACACGAGAAATCCTGATCGCGTCGTTCGCCGGGGTGCTTGGGACGATCACTCTGGCCGGTGTGCTCGCTAACCCGCTGCTCTTGCCGGATGGTAACgaa                                                                                                                                                                                               <  2:226775/247‑3 (MQ=255)                                              cGACCCTAACGTCGAAATCTGGATGCTTGTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCGGGCTGTGGACGATGAAAAAGTTCAGCAACCTCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAAGCCTGATCGCGTTTTTCGCCGGGGTTCGTGGGACGATCACGCTGGCCGGTGTGCTCTCTATCCCGCTGCTCGTGCCGGa                                                                                                                                                                                                         <  2:354001/240‑1 (MQ=255)                                              cGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGCTGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGa                                                                                                                                                                                                         >  1:354001/1‑240 (MQ=255)                                              cGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCt                                                                                                                                                                                              <  1:283309/251‑1 (MQ=255)                                                       cGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGATGGTCCGTTTCGGCTGGCTGTGGAGGATAAAGACGTTCAGCAACCGCTTACTGAAGAAGAAGCCGCTGGCGTTTGGTTCGTGGACCACACTAGAAATACAGTTCGCGTCTTTCGACGGGGTGCGTGGGACGATAACTATGGCCGGGGTGCTCTCTATCCCACTGCTCTTGCCGGCTGGTAACGGCGTCCCGgccc                                                                                                                                                                                     >  2:284544/1‑249 (MQ=255)                                                                 tGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTAGACGATGAAACAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGAGGACCACACGAGAAATCCTGATCGCGTATTTCGCAGGGGTGCGTGGGACGATCACTCTGGCCGGGGTGCTCTCTATCCCGCTGCACTTGCCGAATGGCAACGTCTTCCCGGCGCGCTCTGAGCt                                                                                                                                                                           >  1:358423/1‑251 (MQ=255)                                                                    aggatgggtacaaaTATTATTCGGAGATATGCGGCGCGGTTGCTGGTCCGTTGCGGCTGGGGGTAGACGATGAAAAAGTTCAGTAAGCGGTTCGTGAAGAAGAAGCCGATGGTGTGTGGTTCGTGGACTACGCGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGGTCCCTCTGGCTGGTGTGCTCTTTATCCCGCTGCTCTTGCCGTATGGTAACGTGTGCCCGGATCGGTATGAGCTGgt                                                                                                                                                                        <  2:343146/239‑1 (MQ=255)                                                                        tGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCACTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTc                                                                                                                                                                    >  1:84549/1‑251 (MQ=255)                                                                           ttACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCAc                                                                                                                                                                                                                                                <  1:8423/172‑1 (MQ=255)                                                                           ttACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCAc                                                                                                                                                                                                                                                >  2:8423/1‑172 (MQ=255)                                                                             aCCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTggc                                                                                                                                                               >  1:60530/1‑251 (MQ=255)                                                                                            atatatGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGACGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGCGCTGGTGTTCCTGGCGGCTGGCGTCAttct                                                                                                                                                >  1:23727/1‑251 (MQ=255)                                                                                                          gATGCTAGTCCGCTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGCTGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGGCATTCTCTTCCCGCTGTTTg                                                                                                                                  >  2:175268/1‑251 (MQ=255)                                                                                                                 gTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCgg                                                                                                                           >  1:124180/1‑250 (MQ=255)                                                                                                                        tCGGCTGGTTGGGGACGATGAAAAAGTTAAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCCCACGAGAAATCCTGATCGCGGCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGAt                                                                                                                     <  2:263937/250‑1 (MQ=255)                                                                                                                         cGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATg                                                                                                                    <  1:80639/250‑1 (MQ=255)                                                                                                                          ggctggctGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGtt                                                                                                                  <  1:336482/251‑1 (MQ=255)                                                                                                                                  gtgGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAAGCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTAt                                                                                                            <  1:9983/249‑1 (MQ=255)                                                                                                                                     gACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGCGTTCCTGGCGGCTGGCGTCATTCTCTTCCCGCTGTTTGTAGGCgtgggtgtgttggcttttctgc                                                                                                       >  2:81324/1‑233 (MQ=255)                                                                                                                                         aTGAAAAAGGTCAGCATCCGTTTCCTGAAGAAGAAGCCGATGGAGGTGGGGTCGTGGACCACACGAGAATTCCTGATAGCGTCTTTCGCCGGGGTGCGTGGGACGATTACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTTGTGTTCCTGGCGGCTGGTGTCATTCTCTGCTCGCTGTTTGGCGGCGTGGTGATGTTGCCTATTCTGCTaca                                                                                                   <  2:63262/251‑1 (MQ=255)                                                                                                                                               aaGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTTTTCTGCTACAACACAt                                                                                             <  1:378809/251‑1 (MQ=255)                                                                                                                                                 gTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTg                                                                                           >  1:113906/1‑251 (MQ=255)                                                                                                                                                            ccTTCCTGAATAAGAAGCCGGTTGAGTTTGGTTCGTTGACAACACGTGAAATCCTGATGGCGTGTTTCGCCTGGTGGCGTGGTGCGATCACTCTTGGCGGGGTGCTCTCTATCCCGCTGCTCTTGCCGGATTGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGTCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGTCGGGGTGATGTTTCCTATTCTGCTACAACACATTGAAGTCTCGGAt                                                                                <  2:297946/250‑1 (MQ=255)                                                                                                                                                                   gaagaagagGCCTATGGAGTTTGGTTCGTGTACCACACGAGAAATCCTGATCGTGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTc                                                                           <  2:62287/249‑1 (MQ=255)                                                                                                                                                                      gaagaagCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGcaac                                                                      >  1:176536/1‑251 (MQ=255)                                                                                                                                                                           agCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTg                                                                 <  1:301019/251‑1 (MQ=255)                                                                                                                                                                              cGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATTAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGAAGTTGCCTATTCTTCTAAAACACATTGAAGTCGCGGAACATTCGCAACAATTGaa                                                               >  2:321330/1‑250 (MQ=255)                                                                                                                                                                              cGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGaaa                                                              <  1:55513/251‑1 (MQ=255)                                                                                                                                                                                    aGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGaa                                                        <  1:118257/251‑1 (MQ=255)                                                                                                                                                                                    aGTTTGGTTCGGGGACCACACGATAAATCCTGATCGCGTCTTTCGCCGGGGTGCGCTGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTTGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGaa                                                        <  1:56237/251‑1 (MQ=255)                                                                                                                                                                                                ggACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGcgcg                                             <  1:81324/250‑1 (MQ=255)                                                                                                                                                                                                                ccTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTTGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAAGTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGaa                             <  1:29817/250‑1 (MQ=255)                                                                                                                                                                                                                                gCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGAt            <  1:48384/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATc           >  1:174534/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTGGGACGTTCACTCTGGCCGGTGTGCTCTCTATCCCGCTTCTCTTGCCGGTTGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCGACAACACATTGAAGTCGCGGAGCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATg  <  2:266068/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      GCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGGCGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATG  >  NC_000913/4280855‑4281338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑