Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,226,699	G→A	G958R (GGG→AGG)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,226,699	0	G	A	87.0%	98.2 / 6.9	46	G958R (GGG→AGG)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (16/24);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCAGCGTGATGATTTTGTCGCTCGTACCCGCAAGGAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑GGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCT  >  NC_000913/4226449‑4226944                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      ccAGCGTGATGATTTTGTCGCTCGTACCCGCAAGGAGTACGAAACCGTACGTATGCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑g                                                                                                                                                                                                                                                       <  1:36424/251‑1 (MQ=255)     cGTGATGATTTTGTCGCTCGTACCCGCAAGGAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCCCCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTGCTTTATGACCTGGTCGCTGGCC‑AGGaa                                                                                                                                                                                                                                                   <  2:83498/251‑1 (MQ=255)      gTGATGATTTTGTCGCTCGTACCCGCAAGGAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGa                                                                                                                                                                                                                                                    >  1:94666/1‑249 (MQ=255)      gTGATGATTTTGTCGCTCGTACCCGCAAGGAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGAGATAACGATTTCGCTTTTGACTGGCAGGCGTACACGCCGCCGGTGGCGCACCGGCTCCGCGGTCCGGAAGTCGAAGCAAGCATCGCAACGCTGCGTCATTACATCGGCTGGACCACGATCTTTATGACCTGGGCGATGGCA‑gggg                                                                                                                                                                                                                                                    >  2:166864/1‑248 (MQ=255)                    gCTCGTACCCGCAAGGAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCt                                                                                                                                                                                                                                    <  1:53544/251‑1 (MQ=255)                               cAAGGAGTAAGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTAACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGACGGTGGCGCACCGTCTAGGCGTGCAGGGAGTCGAAGCCAGAATCGAAACGCTGCGGAATTACATCGCCTGGACACCGTTCTTTATGAACTGGTAGCTGGCCCGGGAA‑TATCCGCGCATTCTGGAAGGTGa                                                                                                                                                                                                                           >  2:330819/1‑249 (MQ=255)                                   gAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGGATAGGCGTGCAGGAAGTCGAAGCCAGCATCGCCACGCTGCGTAATTACATCGACTGGACACCGATCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCAGTCTGGAAGATGAAgcggt                                                                                                                                                                                                                     >  2:104214/1‑251 (MQ=255)                                     gTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACCTCGACTGGACACCGTTATTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGtggtgg                                                                                                                                                                                                                   >  1:317335/1‑251 (MQ=255)                                     gTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGtggtgg                                                                                                                                                                                                                   >  1:224384/1‑251 (MQ=255)                                         gAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGt                                                                                                                                                                                                               <  1:296389/251‑1 (MQ=255)                                         aaaaCCGAACGTATACAGCACGGGCGCGAGAACACGCGCGTACCACCGTGCACGTTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGAGGAAGTGGTGGGCGt                                                                                                                                                                                                               <  2:140896/250‑1 (MQ=255)                                                   gtaTTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGAGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑GGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAg                                                                                                                                                                                                     <  1:166864/251‑1 (MQ=255)                                                      ttCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAGGCCAGCATCGAAACGCTGCGTACTTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATACGCGCATTCTGGAAGATGTAGTGGTGGGCGTTGAGGCGCAGCgg                                                                                                                                                                                                  >  1:286045/1‑251 (MQ=255)                                                          gCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGt                                                                                                                                                                                              <  1:185974/251‑1 (MQ=255)                                                                              gcACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGGAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATg                                                                                                                                                                          <  1:330819/251‑1 (MQ=255)                                                                                     accGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGAt                                                                                                                                                                    >  1:4485/1‑250 (MQ=255)                                                                                         gTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTCGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAAtt                                                                                                                                                               >  1:138093/1‑251 (MQ=255)                                                                                                 ggAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTGACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAgcgc                                                                                                                                                         <  1:64689/249‑1 (MQ=255)                                                                                                       ggcgCGGGGTAACGATTTCGCTTTTGTCGGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAATCGCTGCGTAATTACATCGACTGGACTCCGTTCTTTATGACCTTGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGaaaa                                                                                                                                                 <  2:229178/251‑1 (MQ=255)                                                                                                        gcgcgcGAGAACGATTTCGTGTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGGCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAAc                                                                                                                                                <  2:105641/251‑1 (MQ=255)                                                                                                           cgcgATAACGATTTCGTTTTTGACTGGCAGGCTAACACGCCGCCTGTGTCGCACAGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACTCCGTTCTTTATGACCTGGTCGCTGTCA‑AGGAAGTATCCGCGAATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCATCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCTAGAATACGCt                                                                                                                                             <  2:355601/251‑1 (MQ=255)                                                                                                             cgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCCTCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCt                                                                                                                                                                                                >  2:37917/1‑198 (MQ=255)                                                                                                             cgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCt                                                                                                                                                                                                <  1:37917/198‑1 (MQ=255)                                                                                                                            ttttGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGcgtggcgtgg                                                                                                                            >  1:77086/1‑251 (MQ=255)                                                                                                                             tttGACTTGCAGGCTTTCACGCCGCCGGTGGCGCACCGGTTCGGCGTGCAGGAAGGCGAAGCCAGCATCGAAACGCTGCGTAATTACAACGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGcgtggcgtgg                                                                                                                            <  2:113386/250‑1 (MQ=255)                                                                                                                                 aCTGGCAGGATTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGGCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAGGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCGGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGGTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGCAAATGCTGAATCCGCGTGGCGTGGTGGGc                                                                                                                       <  1:278343/251‑1 (MQ=255)                                                                                                                                   gggCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCt                                                                                                                     <  1:201006/250‑1 (MQ=255)                                                                                                                                       aGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTc                                                                                                                 >  1:149787/1‑251 (MQ=255)                                                                                                                                         gCTTACAGGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTccc                                                                                                               <  1:274669/251‑1 (MQ=255)                                                                                                                                                 gccgccGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑GGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAAcc                                                                                                       <  1:171613/251‑1 (MQ=255)                                                                                                                                                                       gtcGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTGGAGGCGCAGCGGCTGTTTAAAGACGCCAACGTCATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTTGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAAt                                                                                 <  1:359702/249‑1 (MQ=255)                                                                                                                                                                         cGTGCAGGAAGTCGAAGCCAGCATCGAAAAGCTGCGTAATTACCTCGACTGGACACAGTTCTTTATGACCTGGTAGCTGGCC‑AGGAAGTATCCGCGCATTATGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCt                                                                               >  1:327884/1‑251 (MQ=255)                                                                                                                                                                                gAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTg                                                                         >  1:109288/1‑250 (MQ=255)                                                                                                                                                                                gAAGACGAAGCCAGCTCCGAAACGCTGCGGAATTACATCGACGTGACACCGTTCTTTATGACCTGGGCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATGAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGa                                                                        <  2:6685/251‑1 (MQ=255)                                                                                                                                                                                  aGTCGATGCCAGCATCGAAACGCGTCGTAATTACAGCGACGTGACACCGTTCTGTATGTCCCGGTCGCTGGCC‑AGGAAGTACCCGCGCATTCTGGAAGAGGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATGAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACg                                                                      <  2:128170/251‑1 (MQ=255)                                                                                                                                                                                           cAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGt                                                              >  1:1926/1‑250 (MQ=255)                                                                                                                                                                                              cATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTAccc                                                          >  1:301386/1‑251 (MQ=255)                                                                                                                                                                                                tCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑GGGAAGTGTCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAGGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGAAGACATTGAAATCTACCGTGACGAACCGCGTaagctc                                                        >  2:129346/1‑246 (MQ=255)                                                                                                                                                                                                tCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑GGGAAGTGTCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTa                                                             <  1:129346/246‑1 (MQ=255)                                                                                                                                                                                                       gCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGATGGCC‑GGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGCGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTAACGAAACGCGTACCCATGTGATc                                                  >  1:66878/1‑250 (MQ=255)                                                                                                                                                                                                                   aagctaCTGGACTCCGTTCTTTATGTCCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATGCTGGAAGATGAAGTGGTTGGCGTTGAGGCGCAGCTGCTGTTTAAAGTCGCCAACGACATGCTGGATAACTTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGAGTGGGCGATGACATTGAAATCTACCGTGACTAAACGCGTACCCATGGGATCAATGTCAGccacc                                     <  2:320503/246‑1 (MQ=255)                                                                                                                                                                                                                          tGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGTGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGa                                                                                    <  1:197867/197‑1 (MQ=255)                                                                                                                                                                                                                                 cGTTCTTTATGACCTGGTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAg                       <  2:4485/251‑1 (MQ=255)                                                                                                                                                                                                                                          agcacTGGGCGCTGGCC‑AGTAAGGATCCGCGCATTCTGGACTATGAAGTGGTGGGCGGTGAGGCGCAGCGGCTGTTTAAAGCCGCCAACGACATGCTGGATATATTAAGCGCCGAGAAAACGCTGAATCCGCATGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGaaaaa              <  1:11716/247‑1 (MQ=255)                                                                                                                                                                                                                                                ggTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAgg         <  1:25993/250‑1 (MQ=255)                                                                                                                                                                                                                                                ggTCGCTGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGc        >  1:158227/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gcGCTGGCC‑AGGAAGTATCCGCGCAGTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCTCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCtt      <  2:94666/250‑1 (MQ=255)                                                                                                                                                                                                                                                      tGGCC‑AGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCt  >  1:24320/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CCAGCGTGATGATTTTGTCGCTCGTACCCGCAAGGAGTACGAAACCGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC‑GGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAAGACGCCAACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCT  >  NC_000913/4226449‑4226944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑