Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,133,252 C→T P213S (CCG→TCG)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,133,2520CT88.9% 36.1 / ‑4.8 18P213S (CCG→TCG) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (5/11);  total (5/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTG  >  NC_000913/4133018‑4133498
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                        
cgggTGACGCTGGTCAAAGAAGTGGCAGATTACGAGGTCTCCGTGGCGGCGTATCCGTAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGACTTGCTTAATCTGAAACGAAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAAGTATT‑CCGGGAATTTTGTCGGTATCTAACTTTaa                                                                                                                                                                                                                                          <  2:379477/249‑1 (MQ=255)
  ggTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTTCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCTCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAAc                                                                                                                                                                                                                                        <  1:42071/251‑1 (MQ=255)
       cGCTGTTAAAAGAAGTGGCAGATTTCGATTTCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCg                                                                                                                                                                                                                                   <  1:207837/251‑1 (MQ=255)
                               tCGAGATCGCCGTGGGGGCGTATCCGGGAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGCCAGTATCTAACTTTAAACAGGCGAAGAATTTTGCCGATATGACCaa                                                                                                                                                                                                            <  2:194219/250‑1 (MQ=255)
                                 gATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGTT‑GTGGGAATTATTTCCGGGA‑TTTTTTCGGGATCTAACTTTTAACAAGCGAGAAAATTTGCCGGTATGACCAAc                                                                                                                                                                                                           >  1:370608/1‑249 (MQ=255)
                                                    aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGCTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGAT‑GTGGGAATTATT‑TCGGGGATTTTGTCGGGGTCTAACTTTTAAAAGGCGGAGAAATTTGCCGATATGAccccatcgggagagcacacgtctga                                                                                                                                                                                       >  1:128126/1‑227 (MQ=255)
                                                    aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACGTTAAACAGGCGAAGAAATTTGCCGATATGAcc                                                                                                                                                                                                              <  2:128126/227‑1 (MQ=255)
                                                     tcctggaGTTCACCCGGAAGCAAAAAGCCCTCATGCGAAGTTGCTTAATCTGTAACGCAAAGTGGATGACGGAGCTAACCGCTGTG‑TTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTGGCCGATATGACCAACGTGCGTATTCCGGCGTGGATg                                                                                                                                                                                      <  2:298145/244‑1 (MQ=255)
                                                                gcgcggagtcaaaaagtgcctaGGCGTATTTTCTTATTCTGAAACGCATAGAGGATGCCGGAGCCAACCGC‑GGGATTGCTCTTTTCTTCGGCGATGTCTATAGCTACCTGCGTTTTCGTGATCGCTGTTTATCGGCGGGCAGTGAT‑GTGGAAATTATT‑CCGGGAATTTTGCCGGGATTTAACTTTAAACAGGCGAAAAAATTTGCCGATATGTCCAACGTGCGTATTCCGTCGTGGATGGCGCAAATGTTc                                                                                                                                                                          <  2:73440/230‑1 (MQ=255)
                                                                                                           aCGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTATTGGGGAAATT‑TT‑CCGGGAATTTTGTCGGGATTTAACTTTTAAAAAGCGAAAAAATTTGCCAGTATGACCCACGGGCGTATTTCGGCGTGGGTGGGGCAAATGTTTGACGGGCTGGGTGGTGATGGCCGAAACCCCCAAACTgttgggg                                                                                                                               >  1:194067/1‑245 (MQ=255)
                                                                                                                      aTGCCGGAGCCACCCGC‑GCGATTACTCAGTTCTTCTGCGATGTCGAAAGCTTCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑cc                                                                                                                  <  2:179796/251‑1 (MQ=255)
                                                                                                                                     gc‑gcgATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGAT‑GTGGGAATTATT‑TCGGGGATTTTTTCGGTATCTAAATTTTAAAAGGCGAAGAAATTTTCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGGGGCAATGTTCGACGGGCTGGATGATGATG‑CCGAAAAACGCAAACTGGTTGGCGCGGATATTGACCA‑GGATATGGTgg                                                                                                     >  1:122074/1‑248 (MQ=255)
                                                                                                                                                  aGTTCTTCATCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTGAAGATTTTAAGCCGt                                                                                       <  1:105135/251‑1 (MQ=255)
                                                                                                                                                                          aCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACt                                                                 <  1:275959/249‑1 (MQ=255)
                                                                                                                                                                              gCGTTTTCGTGACCGCTGTGTATCGGCGGGCAGTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCta                                                             <  1:293500/249‑1 (MQ=255)
                                                                                                                                                                                ggtttCGGGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACg                                                         <  1:233820/249‑1 (MQ=255)
                                                                                                                                                                                   ttCGTTACCTCTGTGTATCGGCGGGAATTGAT‑GTGGAAATGATT‑GCGGTAATTTTGTCGGTATCTAACTTAAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATGCCGGCGTGGATGGCGCAAATGTGCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTGGGAGCGAATATTT‑CCATGGATATGGTGAAGATTGTAAGCCGTGAAGGAGTGAAAGACTCCCACTGCTATACGCt                                                       <  2:129522/250‑1 (MQ=255)
                                                                                                                                                                                                                                         tttGTCGGTATCTAACTTTAAACAGGCGAAGCAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTAAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTg  >  1:352440/1‑251 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                        
CTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGC‑GCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT‑GTGGAAATTATT‑CCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATG‑CCGAAACCCGCAAACTGGTTGGCGCGAATATTG‑CCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTG  >  NC_000913/4133018‑4133498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑