Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,779	T→C	F155S (TTT→TCT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,779	0	T	C	100.0%	68.4 / NA	22	F155S (TTT→TCT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/12);  total (10/12)

CGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGG  >  NC_000913/3931549‑3931995                                                                                                                                                                                                                                       |                                                                                                                                                                                                                         cGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCTGGCGTAATACGTCGGCGCGAACCTCATCAATGCTGGTTATTATGGGGCTAATCGTCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACCCCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATTCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCa                                                                                                                                                                                                      <  1:156144/251‑1 (MQ=255)   gTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACg                                                                                                                                                                                                    >  1:158245/1‑251 (MQ=255)                   gACGTTGATGTCGCTTGCCGGGCGTAATCCGTCTGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGACTCAAAAACATGGCACGGCTATGGTCGGTAAg                                                                                                                                                                                     <  1:97699/250‑1 (MQ=255)                            gTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTgcgc                                                                                                                                                                           >  1:321474/1‑251 (MQ=255)                              cGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCg                                                                                                                                                                         <  1:286271/251‑1 (MQ=255)                                     cGGGCGTATTACGTCGGCGCGAACCACATCAATGTTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTCTTGTGAAGTCGTCATAACTCCCGCTATTTCGGGGATGTCAGCCATTGCATGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGTTCGGTAAGCTGTTTGCGCCGATc                                                                                                                                                                      <  2:210004/247‑1 (MQ=255)                                     cGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATc                                                                                                                                                                      >  1:210004/1‑247 (MQ=255)                                                          aaCCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGACGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGCTAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTgg                                                                                                                                             >  1:156355/1‑251 (MQ=255)                                                                agcAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATTGTGAAGTCGTCATAACCCCCGCTATTTCGGTGATGTTAGCCATTGAAGGTCTTGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATTGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGAc                                                                                                                                       <  1:154348/249‑1 (MQ=255)                                                                         ggTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTAc                                                                                                                              >  2:282511/1‑251 (MQ=255)                                                                                            ggcggcAGCGTTTTCTATGGTGAAGTCGTCATTACACCCGCTATTTCGGTGATGGCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAAccc                                                                                                           <  2:112831/251‑1 (MQ=255)                                                                                                            aTGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgc                                                                                            <  1:282511/250‑1 (MQ=255)                                                                                                                   aGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATc                                                                                    <  1:357666/251‑1 (MQ=255)                                                                                                                   aGTCGTCATAACACCAGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATCCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATc                                                                                    >  1:127796/1‑251 (MQ=255)                                                                                                                                          cgttGATTTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCATCTCAATTATCGTTCTCACTTTATTATCTATTAGTCAAAAACATGGCACGGCTATGGTCGGTAAGCGGGTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGTATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttctg                                                              <  2:323133/247‑2 (MQ=255)                                                                                                                                                 gTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAAt                                                      >  1:134593/1‑251 (MQ=255)                                                                                                                                                        aTTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAg                                                                                                                          >  2:225733/1‑176 (MQ=255)                                                                                                                                                        aTTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAg                                                                                                                          <  1:225733/176‑1 (MQ=255)                                                                                                                                                                 cTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                                      >  1:150696/1‑251 (MQ=255)                                                                                                                                                                 cTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCAGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                                      >  1:298229/1‑251 (MQ=255)                                                                                                                                                                                 cGCAGCTGGATACCTGGATAGTTCCCCTCTCAAGTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCa                      <  1:292614/251‑1 (MQ=255)                                                                                                                                                                                                     gttacccTCTTAATTATCGTTTTTACTTTATTATCTTTGATTCAAAATCATGGCACTGCTATGGTCGGTAAGCTGTTTGCGCCGTTCATGCTGACCTGGGTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATGAGGGGCAGTGGTGTTGTCGATTACggg  <  2:120045/247‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                         CGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGG  >  NC_000913/3931549‑3931995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑