Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,725,1760TG95.0% 104.4 / ‑4.2 40E48A (GAG→GCG) glyQglycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (15/23);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAAC  >  NC_000913/3724933‑3725417
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
cAGGCCCCCAGGGTCGGGTTTTCCCAGTTCTCTTCCACGAAACGGATGTCGTGAATAGTCGTGTCCATGCCCAGCTCTTTCAGAGACCCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCTATCGGTTCTGGCCCCAGCGCGCGCAg                                                                                                                                                                                                                                             <  2:76315/251‑1 (MQ=255)
        cAGCGTCGGGTTTTCCCAGTTATCTTACACTAAACGGATGTCGTGAATAGTCGGGTCCATGACCGGCTCTTTCAGAGAACCGAGGAACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAACTGGGACTGATAGTAGTGCTGTCAACGGGTAGGGTTTTCGCCGAAGCGACCATCGGTCGGGGGACGAGAACGCTAGACATAAGCAACCGCCATCGGTTCTGGCCCCAGCTCGCGGCGACAG‑GTc                                                                                                                                                                                                                                     >  1:95432/1‑250 (MQ=255)
                          gTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCcg                                                                                                                                                                                                                  >  1:290089/1‑251 (MQ=255)
                                          cGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTc                                                                                                                                                                                                  >  1:133610/1‑251 (MQ=255)
                                                 cGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCATACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATgg                                                                                                                                                                                            <  1:141423/250‑1 (MQ=255)
                                                  gTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGtt                                                                                                                                                                                          >  1:125125/1‑251 (MQ=255)
                                                     aaTAGTCGGGTCAAAGCCAAGCTCTTTCAGAGCACCGAGGTACAACTCCTGAATACTGTCCGGCAATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAACCGGTTGGGGTTTTCGCAGTAGCGACCATCGGACGGGCTACGAGAAGACTGAACATAAGAAGCCGCCCTCGGTTCTGGCCCCAGAGCGCGCAGACAG‑TACATTGGGGGACAGGTTCCGGAGCCGACTTCCATGTCCAATGGTTGac                                                                                                                                                                                       >  2:237304/1‑250 (MQ=255)
                                                      aTAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTTTCCGGCGATGGCTTAATGAACACCTGGAACTGATCGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCCGTCGGGCGACGGGAAGGCTGAACCTAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGGGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGaa                                                                                                                                                                                       >  1:351947/1‑250 (MQ=255)
                                                        aGTCGGGTCCATGCCCAGCTCGTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAACGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAG‑GTCATTGGGTGAGTGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACa                                                                                                                                                                                     <  1:125680/250‑1 (MQ=255)
                                                            gggTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGt                                                                                                                                                                                >  1:158470/1‑251 (MQ=255)
                                                             ggTCCATGCCCAGCACGTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACAACCTGGAACTGATAGTAATGCTGAAAACGGTTGGGATTTTAGCCGTAGCGACCATCGGTCGGGCGACGAGACGGCTGAACATACGAAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GCAATTGGGTGAGAGGTTCCCTCGCCGACTTCAATGTCAAATGGTTTGACAATGGTg                                                                                                                                                                               >  2:254428/1‑251 (MQ=255)
                                                                ccATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAg                                                                                                                                                                            <  1:197821/251‑1 (MQ=255)
                                                                 cATGCCCAGCTCTTTCAGAGAACCTAGGTTCAGCTCCTGAATATTGTCCGGCGATGGCGTAATGGCCCCCCGGAACGGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCATCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAga                                                                                                                                                                           <  2:91143/251‑2 (MQ=255)
                                                                 cATGCCAAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGACTATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGAGCTGTAAACGGTTGGGGTATTCGCCGTAGCGACCCTCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCAGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGACGACTTCCATGTCCACTGGTTGAACAATGGTGCAGc                                                                                                                                                                           >  2:153322/1‑251 (MQ=255)
                                                                            ctTTCAGAGAACCGAGGAACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCATGGCGAGcc                                                                                                                                                                >  1:17703/1‑251 (MQ=255)
                                                                                      accgagttaaagtccatgaaaagtGTCCGGCGTTGGCTTAATGACCCCCGGGAACTGATAGGAGGGCTGTAAACGGTGGTGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACAGAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATCGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCt                                                                                                                                                      <  2:86857/228‑1 (MQ=255)
                                                                                         gatgTACAGCTCCTGAATATTGTGCTGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGATAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTa                                                                                                                                                   <  2:181704/248‑1 (MQ=255)
                                                                                             tACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGAAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCACTGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTCATCCTGTAAGGt                                                                                                                                               >  1:243826/1‑251 (MQ=255)
                                                                                              aCAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCCCCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGAGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTc                                                                                                                                              <  1:294754/251‑1 (MQ=255)
                                                                                                              gTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGTGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTgg                                                                                                                               <  1:259275/250‑1 (MQ=255)
                                                                                                                     gagggCTTAAAGACCTCCTGGAACTGAGAGTAGTGCTGTAAACGGTTGGGTGTTTCGCTGTAGCGACCATCTGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGTTTCTGGCCCTAGTGCGCGGAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAAATATGGTGCAGCCTTGGCGAGCTCAGTAATCCTGTAAGGGCAGGATGAAGCCCTGGAAGGTCCt                                                                                                                       <  2:243826/248‑1 (MQ=255)
                                                                                                                       tGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTgg                                                                                                                     >  1:61059/1‑251 (MQ=255)
                                                                                                                        ggCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGt                                                                                                                    <  1:202577/251‑1 (MQ=255)
                                                                                                                                gACCACCTGGACCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAAc                                                                                                             <  1:44850/250‑1 (MQ=255)
                                                                                                                                  ccccttggacttgatagtcaggCTGTAAACGGTTGGGGTGTTCGCCGTAGCGACCCACGGTCGGGAGACGAGAAGGCTGATCGTAAGCAGCCGCCAGCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCAGTGGGTTAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGGCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACttt                                                                                                          <  2:235741/230‑1 (MQ=255)
                                                                                                                                  ccaccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACttt                                                                                                          <  1:75279/251‑1 (MQ=255)
                                                                                                                                                 agtagtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGt                                                                                           <  1:119502/251‑1 (MQ=255)
                                                                                                                                                  gtagtgcttacaaCGGTTGTGGTTTTCGCCGGTGCGTCCATCGGTAGGTCGACGAGAAGGCTGAACATAAGCCGTCGCTATCGGTTCTGGTCCAAGCGCGCGCAGATAG‑GTCAGTGGGTTAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTTTAAGGTCATGTTCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTg                                                                                          <  2:236351/240‑1 (MQ=255)
                                                                                                                                                          gTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGAt                                                                                    <  1:237304/249‑1 (MQ=255)
                                                                                                                                                          gTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATa                                                                                   <  1:333463/250‑1 (MQ=255)
                                                                                                                                                            aaaCGGTTGGGGTTTTCGCCGTAGCGACCAACTGCCGGGCGACGAGAAGGCGGAACATAAGCGGCGGCCATCGGTTCTGGCCCAAGCGCGGGCAGACAGTGT‑TTTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCTCAGTAATCCTGTAAGGTCAGGATCAAGACATGGAAGGTCCTGGTATCAACCTTTTGCATATTATTTCGTggaagatatc                                                                                 <  2:290089/250‑10 (MQ=255)
                                                                                                                                                             aaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTTCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACgtg                                                                               >  1:285961/1‑251 (MQ=255)
                                                                                                                                                             aaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACgtg                                                                               <  1:218749/251‑1 (MQ=255)
                                                                                                                                                                                      aCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGccc                                                                                                                                  >  1:162445/1‑175 (MQ=255)
                                                                                                                                                                                           cggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc                                                                                                                                                                              >  2:147922/1‑126 (MQ=255)
                                                                                                                                                                                           cggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc                                                                                                                                                                              <  1:147922/126‑1 (MQ=255)
                                                                                                                                                                                                           aGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGAtata                                 <  1:168353/251‑1 (MQ=255)
                                                                                                                                                                                                                 aaCATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTAc                           <  1:3176/251‑1 (MQ=255)
                                                                                                                                                                                                                      aaataatggcatcgacattacgCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCa                                                          <  1:105208/194‑1 (MQ=255)
                                                                                                                                                                                                                                          ggCCCCAGCGCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAAc  >  1:207896/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
CAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAG‑GTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAAC  >  NC_000913/3724933‑3725417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑