Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,707,883 G→A intergenic (‑178/+733) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,707,8830GA96.0% 65.7 / ‑4.2 26intergenic (‑178/+733)dppA/proKdipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/15);  total (10/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTT  >  NC_000913/3707651‑3708057
                                                                                                                                                                                                                                        |                                                                                                                                                                              
ccaccaGGCGGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTCTGAGGTGGGGATGCCGTACTAATTAACATAAGTGTAACAGGGCGGATCCTCATTAAAAGGACCCTCGTGACCTCCAATATATCAACAGCATGTGAAAAc                                                                                                                                                              >  2:169461/1‑251 (MQ=255)
     aGGCTGAGACCAAGCTTCAGCCTACCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCACTCGTGACCTACAATCTATCAACAGAATGCGAAAACGTCa                                                                                                                                                          >  2:244108/1‑250 (MQ=255)
             aCCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGcc                                                                                                                                                  >  1:173858/1‑250 (MQ=255)
                   cTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATAAACCGAATGTGCAAACGTCAATACAGACAACCggg                                                                                                                                           >  1:243967/1‑251 (MQ=255)
                            cccTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACAcc                                                                                                                                  <  1:121065/251‑1 (MQ=255)
                                        taaGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTTACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGaa                                                                                                                       <  1:49778/249‑1 (MQ=255)
                                                 aCGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcacaaaca                                                                                                             <  1:169461/251‑1 (MQ=255)
                                                   gCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCAcaaacaaa                                                                                                           <  1:246661/251‑1 (MQ=255)
                                                       tattcctccccaaatgggaggtttgttGTTTTAGCCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTGTTTGTTTTTTGCCCGGTCGGGTCAACGTTGTGAGGTGGGGATGCCGTACTAATTAGCATCAGGGTAACAGTGCGGAGCCTCCATAAAATGCCCCTCGGGACCTACAATCTGTCAACAGAGTGTGAAAACGGCCATAGAGCCGACCGGGATTTACACCATCAGTGAGAATCAACACACAAAGaa                                                                                                        <  2:46195/230‑2 (MQ=255)
                                                        attCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAtt                                                                                                       <  1:244108/250‑1 (MQ=255)
                                                                   aTTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAATGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACTGTGCGGATCCTCCATAAATTCCCCCTCGTGACCTACAATTTATCAACAGAATGTGAAAACGTCAATACCGACAACCGGGATTTACACAAACGGTGCGAATACACAAATAAAGAATaaaaaatatta                                                                                            >  1:23858/1‑245 (MQ=255)
                                                                     tgtgATGTTTTTTGTTTTACCCCTTTGCAGTGGGTTGTCTCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCATTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAATCAAAGATTAAAAAAACTTCaa                                                                                          <  2:361947/250‑1 (MQ=255)
                                                                                  gttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgca                                                                             <  1:319597/250‑1 (MQ=255)
                                                                                   ttttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACATTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAACCTTAAAAAAGCTATTTgcagc                                                                           >  2:161670/1‑251 (MQ=255)
                                                                                    tttAACCCTTTGCAGTTGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAATCAGCTATTTgcagc                                                                           <  1:281925/250‑1 (MQ=255)
                                                                                                cAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgtg                                                              <  1:100739/251‑1 (MQ=255)
                                                                                                               gCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAc                                               <  1:161670/251‑1 (MQ=255)
                                                                                                                               tttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                <  1:322432/250‑1 (MQ=255)
                                                                                                                                  ttgCCCGGTCGGGTAAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAACCAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAat                             >  2:148990/1‑250 (MQ=255)
                                                                                                                                   tgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAata                            <  1:297490/250‑1 (MQ=255)
                                                                                                                                   tgCCCGGTCGGGACAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACCGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCCACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAatat                           >  1:79803/1‑251 (MQ=255)
                                                                                                                                       aggacggatCAACGTTATGCGGTAGGGATGCCGTACTAATTAACATCAGTGAAACAGTGCGGATCCTCCATAAAATGCACCTCGTGACCTACAATATATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCCAAAACAAAGATTAAAACAACTTCAACCAACTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACAAGAACAATCAGCTTAATAttt                         >  2:355952/9‑249 (MQ=255)
                                                                                                                                                 aaCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATc                                     <  1:9/227‑1 (MQ=255)
                                                                                                                                                 aaCGTTATGAGGAGGTGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTAACAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAAACCACAAACAAAGATTAAAAAAACTTCAAACATCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTgg             <  1:61364/251‑1 (MQ=255)
                                                                                                                                                 aaCGTTATGAGGAGGGGATGCCGTACTAATTAACCTCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTCTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGATAATCCACACAAAAAGCTTAAAAAAACTTCACCCAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATTACCAGAACAATCAgatcgacagagcgtcttgtaggg             >  2:9/1‑229 (MQ=255)
                                                                                                                                                            gTGGGGAGGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTGACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGttt  <  1:379977/251‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                              
CCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTT  >  NC_000913/3707651‑3708057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑